Human osteoblast cell spreading and vinculin expression upon biomaterial surfaces

Abstract

Any biomaterial implanted within the human body is influenced by the interactions that take place between its surface and the surrounding biological milieu. These interactions are known to influence the tissue interface dynamic, and thus act to emphasize the need to study cell-surface interactions as part of any biomaterial design process. The work described here investigates the relationship between human osteoblast attachment, spreading and focal contact formation on selected surfaces using immunostaining and digital image processing for vinculin, a key focal adhesion component. Our observations show that a relationship exists between levels of cell attachment, the degree of vinculin-associated plaque formation and biocompatibility. It also suggests that cell adhesion is not indicative of how supportive a substrate is to cell spreading, and that cell spreading does not correlate with focal contact formation.

In vitro cellular response and in vivo primary osteointegration of electrochemically modified titanium

“…Our study confirms that the morphology of cells grown on titanium can reveal their interactions with the surface, especially in the early phases of culture, i.e., attachment and adhesion [45,55,56]. Concerning the cell attachment and adhesion morphological analysis performed by SEM, each tested surface exhibited specific characteristic cell-material interaction.…”

A Comparative Study on In Vitro Osteogenic Priming Potential of Electron Spun Scaffold PLLA/HA/Col, PLLA/HA, and PLLA/Col for Tissue Engineering Application

“…Previous studies have suggested that the rounded appearance of cells could be due to the actin bundles or stress fibers [22]. An interesting observation in the present study was the roughness of the material, which was notably changed after cell growth; particularly, the RMS and average roughness after cell seeding were decreased by twofold in the case of PLLA/Col/HA scaffold (0.293 and 0.218 µm, respectively), when compared with those in the case of other scaffolds ( Figure 8A, B, and C ).…”

The Role of Titanium Surface Nanostructuring on Preosteoblast Morphology, Adhesion, and Migration

“…The software automatically detects the cell outline and calculates parameters such as the number of cells per unit area (cell density [cells mm −2 ]) and cell area per unit area of the substrate (cell coverage [%]). [71,72] Cell Proliferation: MC3T3-E1 cells were seeded onto the biomaterial surfaces at the density of 6000 cells cm −2 with α-MEM medium (Invitrogen) containing 10% FCS. Cells were fixed in 4% paraformaldehyde (PFA) at the respective time points (3 h, 6 h, 1 d, and 2 d), permeabilized with 0.5% Triton-X (Sigma) in phosphate-buffered saline (PBS) for 5 min, and blocked with PBS containing 3% Normal Goat Serum (Sigma) and 2% bovine serum albumin (BSA) (Sigma) for 1 h. Cells were then washed once with TBS-T buffer.…”