Human papillomavirus 16 E6 modulates the expression of miR-496 in oropharyngeal cancer

Mason, Dayna; Zhang, Xiaoying; Marques, Tânia Monteiro; Rose, Barbara; Khoury, Samantha; Hill, Meredith; Deutsch, Fiona; Lyons, J. Guy; Gama‐Carvalho, Margarida; Tran, Nham

doi:10.1016/j.virol.2018.05.022

Cited by 15 publications

(6 citation statements)

References 46 publications

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“…Previous studies have indicated that miR-496 is associated with several human pathological changes, including oropharyngeal cancer (27), cerebral ischemia/reperfusion injury (28), type 2 diabetes mellitus and obesity (29), and osteogenesis of human bone marrow (30). These findings suggest that miR-496 serves as a suppressor in human pathological changes, which is consistent with our findings.…”

Section: Discussionsupporting

confidence: 92%

MicroRNA‑496 suppresses tumor cell proliferation by targeting BDNF in osteosarcoma

Xie

Zuo

et al. 2019

Exp Ther Med

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MicroRNAs (miRNAs) are integrally involved in biological and pathobiological development. Many studies have demonstrated the abnormal expression of microRNA-496 (miR-496) in various human malignant tumors. The present study was designed to investigate the functions and the underlying mechanisms of miR-496 in osteosarcoma (OS) progression. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to determine the expression of miR-496 in OS tissues and cell lines. Luciferase activity was used to confirm the interaction between miR-496 and brain derived neurotrophic factor (BDNF), a downstream gene of miR-496. RT-qPCR was also used to quantify BDNF mRNA expression, and the BDNF protein expression level was detected by western blot analysis. In addition, the Cell Counting Kit-8 (CCK-8) was used to detect cell viability. The results revealed that the level of miR-496 expression was significantly reduced in osteosarcoma tissues and cell lines. BDNF was verified to be a direct target gene of miR-496 and was found to be negatively regulated by miR-496. Overall, it was demonstrated that miR-496 inhibits osteosarcoma cell proliferation via inhibition of BDNF. Thus, the miR-496/BDNF axis may be a novel strategy for the clinical treatment of OS.

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Section: Discussionsupporting

confidence: 92%

MicroRNA‑496 suppresses tumor cell proliferation by targeting BDNF in osteosarcoma

Xie

Zuo

et al. 2019

Exp Ther Med

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“…MiRNAs can target or control the expression of tumor-related genes, thereby promoting or inhibiting the occurrence and progression of tumor (Sun et al, 2018 (Dmitriev et al, 2013;Lu et al, 2018;Mason et al, 2018). A study reported long noncoding RNA-DANCR contributes to lung adenocarcinoma progression by sponging miR-496 to modulate mTOR expression (Lu et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…MiR-496 was recently found to be a novel mammalian target of rapamycin (mTOR) regulator associated with aging-relevance (Rubie et al, 2016). Besides, miR-496 level has been reported to be deregulated in several diseases, such as facioscapulohumeral muscular dystrophy, age-related diseases, and tumor progression (Dmitriev et al, 2013;Lu et al, 2018;Mason et al, 2018). A study reported long noncoding RNA-DANCR contributes to lung adenocarcinoma progression by sponging miR-496 to modulate mTOR expression (Lu et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

MiR‐496 promotes migration and epithelial‐mesenchymal transition by targeting RASSF6 in colorectal cancer

Wang

Yan

Zhang

et al. 2019

Journal Cellular Physiology

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Aberrant loss of tumor-suppressor genes plays a crucial role in tumorigenesis and development of colorectal cancer (CRC). Extensive studies have reported tha hypermethylation of Ras association domain family member 6 (RASSF6) is common in various solid tumors. Another important mode of epigenetic regulation, microRNA (miRNA) regulation of RASSF6, is far from clear. The aim of the present work was to screen out novel miRNA regulating RASSF6, and to explore its underlying mechanism in CRC. With the use of bioinformatics, clinical sample data, and luciferase binding assay, we determined that microRNA-496 (miR-496) could be a novel oncomiR that directly binds to RASSF6. Next, a series of miR-496 mimics or inhibitor, or RASSF6 small interfering RNA (siRNA) introduced into CRC cells were applied to examine the effect of miR-496 on CRC cell viability, migration, and epithelial-mesenchymal transition (EMT). The results demonstrated that miR-496/RASSF6 could promote cell migration and EMT via Wnt signaling activation, but had no effect on cell viability.Our results confirmed that the miR-496/RASSF6 axis is involved in Wnt pathwaymediated tumor metastasis, highlighting its potential as a therapeutic target for CRC. K E Y W O R D S colorectal cancer, epithelial-mesenchymal transition, microRNA-496, migration, Wnt signaling pathway

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“…The limited associations observed between IHC markers and HNSCC are consistent with the research conducted by Hashmi et al (2018) [ 19 ]. The function of miRNAs, HPV association, and genetic variation have been explored in several HNSCC-related studies, Sais et al (2018), Momi et al (2014), Miller et al (2015), and Wilkins et al (2018) [ 20 - 23 ].…”

Section: Discussionmentioning

confidence: 99%

The Relationship of Grade, Stage and Tobacco Usage in Head and Neck Squamous Cell Carcinoma With p53, PIK3CA and MicroRNA Profiles

Kiran,

Chowdhury,

Singh

et al. 2024

Cureus

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Background: Head and neck squamous cell carcinoma (HNSCC) has multiple epigenetic modifications including post-transcriptional regulation by microRNAs (miRNAs) as well as alterations in molecular pathways due to mutations. Examining these miRNAs and location-specific molecular alterations is essential to understanding the intricacies of HNSCC and directing focused diagnoses and treatments. Aim: To investigate tobacco-related changes in the expression of miRNAs and proteins with clinicopathological parameters of HNSCC and disease-modifying personal habits like tobacco and alcohol use. Methodology: The study concentrated on oropharyngeal cancers using immunohistochemistry and reverse transcription-polymerase chain reaction. Expression of microRNAs mir15a, mir20b, mir21, mir31, mir33b, mir146a, mir155, mir218, mir363 and mir497 and immunohistochemical expression of P53 and PIK3CA were correlated with grade, stage and personal habits like tobacco and alcohol intake. Results: mir21 and mir15a are under-expressed in higher grades with a trend towards statistical significance (P-value of 0.094 and 0.056 by one-way analysis of variance (ANOVA) on ΔC T values). mir155 and mir146a are overexpressed in stage IV tumours while mir 31 is under-expressed in stage IV tumours but statistical significance was not reached. mir497 showed overexpression in tobacco users, but these results were limited by many tumours not showing any amplification for the miRNA and statistical significance was not reached. There was no statistically significant association found between immunohistochemical expression of p53 and PIK3CA with grade, stage or personal habits. Conclusion: Through the deciphering of complex miRNA patterns and their relationships with clinicopathology, this study attempted to increase our understanding of HNSCC. Some candidate miRNAs showing probable association with grade, stage and personal habits were identified, but larger studies are needed to confirm or refute the importance of these miRNAs.

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Human papillomavirus 16 E6 modulates the expression of miR-496 in oropharyngeal cancer

Cited by 15 publications

References 46 publications

MicroRNA‑496 suppresses tumor cell proliferation by targeting BDNF in osteosarcoma

MicroRNA‑496 suppresses tumor cell proliferation by targeting BDNF in osteosarcoma

MiR‐496 promotes migration and epithelial‐mesenchymal transition by targeting RASSF6 in colorectal cancer

The Relationship of Grade, Stage and Tobacco Usage in Head and Neck Squamous Cell Carcinoma With p53, PIK3CA and MicroRNA Profiles

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