Human papillomavirus 6, 11, and 16 in laryngeal papillomas

Abstract

Twenty-seven cases of benign laryngeal papillomas, both single and multiple variants, were analysed for human papillomavirus (HPV) by DNA slot-blot hybridization chiefly to determine the pattern of infection in Hong Kong Chinese. DNA was extracted from paraffin blocks of formalin-fixed tissue and probed separately for HPV 6, 11, 16, and 18. Sixteen cases (59 per cent) showed the presence of at least one of these four HPV genomes. Thirteen cases (48 per cent) were positive for HPV 11 only. Three other cases (11 per cent) showed triple positivity for HPV 6, 11, and 16. None were positive for HPV 18. The predominance of HPV 11 infection contrasts with other series which have shown either an almost equal distribution of HPV 6 and 11 or a predominance of HPV 6. The finding of HPV 16 in three cases was unexpected. Using the polymerase chain reaction (PCR) with primers complementary to the upstream regulatory region of the HPV 16 viral DNA, the presence of HPV 16 genome was confirmed in all three cases. As the number of HPV 16-positive cases in this study is small, analysis of more cases using fresh biopsy material and a wider range of HPV type-specific PCR primers is warranted to determine the relative incidence of HPV subtypes in these benign laryngeal papillomas.

Demonstration of multiple HPV types in laryngeal premalignant lesions using polymerase chain reaction and immunohistochemistry

“…Multiple HPV infections, containing two or three types, were detected in 17 of the 28 HPV-positive lesions (60%). HPV 11 was the predominant type, in general agreement with other studies showing a similar frequency in laryngeal papillomas [Dickens et al, 1991]. HPV 16 was detected in 5 of 21 keratosis (23%), 3 of 12 mild dysplasias (25%), 3 of 6 moderate (50%), and 3 of 11 severe dysplasias (27%) ( Table II).…”

Molecular Identification of 7 Human Papillomavirus Types in Recurrent Respiratory Papillomatosis

“…12,14 By means of polymerase chain reaction, our study allowed the detection of HPV DNA in 100% of the analyzed samples. With the use of the restriction enzyme RsaI, HPV types 6,11,16,31,33,35, and 39 were identified by the different 188-bp fragment cuts.…”

Whole Genome Analysis of Human Papillomavirus Genotype 11 from Cervix, Larynx and Lung

“…The risk factors for malignant progression are smoking, radiotherapy, alcohol consumption and specific HPV genotypes. The low-risk HPV genotypes, HPV6 and HPV11 are the major causes of RRP (Dickens et al, 1991) while HPV16 and 18 are less common. HPV6 and 11 are the causative agents of almost 100% of juvenile and adult onset RRP.…”