Human Papillomavirus Distribution among Women in Western Shandong Province, East China using Reverse Blot Hybridization Assay

Abstract

Cervical cancer is the third most common cancer in women worldwide and there is a significant association between human papillomavirus (HPV) infection and cervical cancer. Certain HPV groups, labeled high-risk (HR) HPV groups, are strongly associated with malignancies of the human cervix. HPV prevalence and genotype distribution were analyzed using the REBA HPV-ID ® (YD Diagnostics, Yongin, Korea) assay based on the reverse blot hybridization assay (REBA) with a total of 324 liquid-based cytology samples from women in Western Shandong Province, East China and results were compared with cytological diagnosis. Most of the HPV genotypes that were detected in high-grade cervical lesions were HR-HPV genotypes such as HPV 16, 18, 33, 53, and 58. The prevalence of these HR-HPV genotypes increased in high-grade cervical lesions. However, from low-to high-grade cervical lesions, the ability to detect LR-HPV genotypes decreased. Additionally, in general, the single HPV genotype infection rate increases in proportion to the severity of the lesion. The study findings suggest that a currently available preventive vaccine against HPV 16 and 18 may have limited effectiveness for prevention of all HPV infection in this province. Finally, based on these findings, these data could guide national or regional vaccination programs in the Western Shandong Province of East China to substantially reduce the burden of cervical lesions.

Distribution of Oncogenic Human Papillomavirus Genotypes at High Grade Cervical Lesions above CIN 2 Grade with Histological Diagnosis

“…HPV genotypes distribution is various depending on the country, province and substantial geographical variation (Bruni et al, 2010). In previous studies, HPV 16,18,31,33, and 45 were reported to be the most prevalent HPV genotypes associated with cervical cancer in Europe;HPV 16,18,45,31,and 33 were the most common HPV genotypes in North America;and HPV 16,18,45,33,and 31 were the most common in Western and Central Asia,whereas HPV 16,18,58,52, and 33 were the most prevalent HPV genotypes in Eastern Asia (Korea, China, and Japan) Li et al, 2011;Kim et al, 2012;Lee et al, 2015).…”

HPVE6/E7,hTERT, andKi67mRNA RT-qPCR Assay for Detecting High-Grade Cervical Lesion with Microscope Slides

“…Above all, precancerous lesion samples are relatively infrequent compared to normal samples, and there is no idea how much time needed to collect enough samples for statistical analysis. In fact, HSIL samples, which are the most severe precancerous lesion, were collected over three years in Korea and China, but there were about 50 samples [16, 19]. In contrast, microscope slides are sealed with Canada balsam in a vacuum state, so their storage period is longer relatively.…”

Performance of HPV E6/E7 mRNA Genotyping Test on Paired Cervical Cancer Exfoliated Cells and Formalin Fixed Paraffin Embedded Tissues

“…Original Article performed from cervical cytology specimens and used in natural history studies, implementation of screening programs, and follow-up of vaccination studies (Wheeler, 2010;Tay and Tay, 2011;Lee et al, 2015). However, cytology specimens often contain larger numbers of HPV infections, most of which are thought to be transient infections, whereas it is assumed that a lesion is caused by one genotype (Mehlhorn et al, 2013;Ho et al, 1995;Lee, 2013;Lee et al, 2011).…”