Human papillomavirus DNA sequences and p53 over-expression in laryngeal squamous cell carcinomas in Northeast China

Abstract

One-hundred-two patients with laryngeal squamous cell carcinomas in Northeast China were examined for human papillomavirus (HPV) DNA by the polymerase chain reaction (PCR) coupled with Southern blot hybridization, and for p53 over-expression by immunohistochemical staining. HPV DNAs were found in 60 cases (58.8%). HPV-16, -18, -6, -11, and -33 DNAs were detected in 30 cases, 22 cases, 25 cases, two cases, and one case, respectively. In addition, coinfection either with HPV-6 and -16 or with HPV-6 and -18 was detected in 20 cases (33.3% of HPV DNA-positive cases). p53 over-expression was observed in 60 patients (58.8%). p53 was over-expressed significantly in the poorly-differentiated SCC and in patients with metastasis to lymph nodes (P < 0.05, respectively). Both HPV DNA and p53-expression were positive in 35 patients, and negative in 17 patients. Either HPV DNA or p53-expression were positive in 50 patients (25 cases each). Although p53 was detected in 35 (58.3%) of HPV-positive patients, there was no significant correlation between HPV infection and p53 over-expression in laryngeal squamous cell carcinomas of Northeast China.

Epidemiology of HPV in Head and Neck Cancer

“…The HPV DNA was found in 60 cases (58.8%), being the 22 cases,25 cases, 2 cases and 1 case, respectively. Moreover, co-infection either with HPV-6 and -16 or with HPV-6 and -18 was detected in 20 cases (33.3% of HPV DNA-positive cases) (Ma et al, 1998).…”

An unusual human papillomavirus type 82 detection in laryngeal squamous cell carcinoma: Case report and review of literature

“…A few studies report altogether on approximately 80 patients. [20][21][22][23][24][25][26][27] The prevalence of HPV detection determined by PCR-based techniques for HPV detection in these studies varies from 7% to 59%. The high discrepancy is probably due to differences in number of the examined cases and the methods used in each study.…”

Possible implication of Mdm2 as a prognostic marker in invasive laryngeal carcinoma

“…The relationship between HPV and LSCC has not been well established [65]. The largest studies involving LSCC have indicated that the prevalence of HPV infection ranges from 7.4 to 58.8% [10,17,20,38,44]. In a recent case-control study, very low prevalences of HPV DNA and serum antibodies were observed in cases from central Europe and Latin America [55].…”