Human papillomavirus E6-regulated microRNA-20b promotes invasion in cervical cancer by targeting tissue inhibitor of metalloproteinase 2

Cheng, Yuan; Geng, Li; Zhao, Lijun; Zuo, Peng; Wang, Jianliu

doi:10.3892/mmr.2017.7231

Cited by 34 publications

(25 citation statements)

References 21 publications

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“…miR‐20b‐5p has been described as a core HPV upregulated miRNA because it is consistently upregulated in HPV + OPSCC and cervical cancer tissues . Increased miR‐20b‐5p expression in cervical cancer is dependent on the viral E6 oncoprotein and has been shown to promote cancer cell invasion . However, expression of the normally silent miR‐20b‐5p and other miRNA cluster members (miR‐106a‐5p and miR‐363‐3p) caused a decrease in proliferation in oral squamous cell carcinoma cells, highlighting the cancer‐specific role of certain miRNAs.…”

Section: Discussionmentioning

confidence: 99%

Extracellular vesicle microRNA cargo is correlated with HPV status in oropharyngeal carcinoma

Peacock

Rigby

Bradford

et al. 2018

J Oral Pathology Medicine

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Background: The incidence of human papilloma virus positive (HPV + ) oropharyngeal squamous cell carcinoma (OPSCC) has increased rapidly in recent decades. These tumours have a favourable outcome compared to HPV-negative (HPV − ) OPSCC. However, HPV + tumours are more likely to metastasise to distant sites, suggesting a difference in how these tumour subtypes interact with the metastatic niche. Extracellular vesicles (EVs) have emerged as important players in cell-to-cell communication and are a potential source of biomarkers for cancer diagnosis. This study aims to characterise the microRNA cargo of small EVs released by HPV + and HPV − OPSCC cell lines. Methods: Extracellular vesicles produced by HPV + (SCC2 and SCC90) and HPV − (SCC72 an SCC89) OPSCC cells were characterised by tunable resistive pulse sensing (TRPS) and western blotting. RNA was extracted from EVs and analysed by small RNA sequencing. A bioinformatics approach was used to identify EV miRNA signatures associated with HPV status. Results: HPV − OPSCC cells produced more EVs than HPV + OPSCC cells. EVs were positive for the common EV markers CD63, CD9 and TSG101. Unbiased hierarchical clustering analysis of EV miRNA cargo revealed that samples clustered based on HPV status. 14 miRNA were enriched in HPV + cell-derived EVs, whereas 19 miRNA were enriched in EVs derived from HPV − cell lines. Conclusions: Here, we identify EV miRNA signatures indicative of the HPV status of the parent cell. This may provide a platform from which to validate salivary or bloodbased biomarkers with utility for early detection and stratifying risk in OPSCC patients. K E Y W O R D S extracellular vesicles, head and neck cancer, human papillomavirus, microRNA, oropharyngeal carcinoma

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Section: Discussionmentioning

confidence: 99%

Extracellular vesicle microRNA cargo is correlated with HPV status in oropharyngeal carcinoma

Peacock

Rigby

Bradford

et al. 2018

J Oral Pathology Medicine

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“…The Human papilloma virus (HPV) viral DNA detection was perfomed by CONFIDENCE HPV™ test (GenoID, Budapest, Hungary), which detects HPV16, HPV18 separately, and the other high-risk HPV-types (31,33,35,39,45,51,52,56,58,59, 66 and 68) in group using multiplex real-time polymerase chain reaction technology [20].…”

Section: Hpv Genotyping Testmentioning

confidence: 99%

Increased miR-20b Level in High Grade Cervical Intraepithelial Neoplasia

Szekerczés

Galamb

Varga

et al. 2020

Pathol. Oncol. Res.

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Cervical cancer is a common malignant tumor worldwide ranking fourth in incidence and mortality among females, which was reduced significantly by cytology screening and human papilloma virus (HPV) DNA testing. The specificity of cytology is high; however, the sensitivity is low, in contrast to the HPV DNA testing. Despite the success of these measures, new biomarkers are still considered to aim increasing sensitivity and specificity of screening and diagnosis. Significant alterations in microRNA (miRNA) expression have been detected in several cancers with variable consistency. To investigate the stratification role of miRNAs between normal epithelium and cervical intraepithelial neoplasia (CIN2-3), we screened the expression of 667 miRNAs to identify significant markers (n = 10), out of them 9 miRNAs were applied in the study (miR-20b, −24, −26a, −29b, −99a, −100, −147, −212, −515-3p) along with RNU48 and U6 as the references. To benchmark the miRNAs, 22 paired (tumor-free and tumor tissue pairs) laser microdissection-obtained cervical formalin fixed, paraffin embedded tissue samples were assayed. The expression of miR-20b was 2.4 times higher in CIN2-3 samples as compared to normal tissues (p < 0.0001). In the HPV16-positive subsets of the samples (n = 13), miR-20b showed 2.9-times elevation (p < 0.001), whereas miR-515 was 1.15-times downregulated (p < 0.05) in CIN2-3 as compared to normal tissue. These results suggest the potential value of miR-20b as a statification biomarker in order to differentiate neoplastic and non-tumorous cases.

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“…This happens mostly when the attention is focused on the EMT of the host cell, in order to identify perhaps the key passage involved in the cell transformation. For example, hsa-miR-20b, -miR-34a, -miR-218, -miR-29a and -miR-146a have been described as regulated by HPV18 E6/E7 and have been involved in initiation and progression in HPV related cervical cancer [118,119].…”

Section: Cell Transformationmentioning

confidence: 99%

Non-Coding RNAs: Strategy for Viruses’ Offensive

Gallo

Bulati

Miceli

et al. 2020

ncRNA

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The awareness of viruses as a constant threat for human public health is a matter of fact and in this resides the need of understanding the mechanisms they use to trick the host. Viral non-coding RNAs are gaining much value and interest for the potential impact played in host gene regulation, acting as fine tuners of host cellular defense mechanisms. The implicit importance of v-ncRNAs resides first in the limited genomes size of viruses carrying only strictly necessary genomic sequences. The other crucial and appealing characteristic of v-ncRNAs is the non-immunogenicity, making them the perfect expedient to be used in the never-ending virus-host war. In this review, we wish to examine how DNA and RNA viruses have evolved a common strategy and which the crucial host pathways are targeted through v-ncRNAs in order to grant and facilitate their life cycle.

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Human papillomavirus E6-regulated microRNA-20b promotes invasion in cervical cancer by targeting tissue inhibitor of metalloproteinase 2

Cited by 34 publications

References 21 publications

Extracellular vesicle microRNA cargo is correlated with HPV status in oropharyngeal carcinoma

Extracellular vesicle microRNA cargo is correlated with HPV status in oropharyngeal carcinoma

Increased miR-20b Level in High Grade Cervical Intraepithelial Neoplasia

Non-Coding RNAs: Strategy for Viruses’ Offensive

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