Human papillomavirus (HPV) DNA assay as an adjunct to liquid‐based Pap test in the diagnostic triage of women with an abnormal Pap smear

Vassilakos, Pierre; Marval, F. de; Muñoz, M.; Broquet, G; Campana, A.

doi:10.1016/s0020-7292(98)00012-5

Cited by 15 publications

(9 citation statements)

References 14 publications

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“…We believe that they should be considered as complementary diagnostic tools and not as competing methods. Their combination has been shown to increase sensitivity and specificity (Ferenczy et al, 1996;Vassilakos et al, 1998) and this may allow more cost-effective screening strategies and improved patient management Cuzick and Sasieni, 1999;Solomon et al, 2001). …”

Section: Molecular and Cellular Pathologymentioning

confidence: 99%

“…In addition, recent data indicate that HPV testing may be used efficiently in the management of ASCUS cases by referring to colposcopy only those women who test positive Solomon et al, 2001). Used as an adjunct to conventional or liquid-based cytology, HPV DNA testing has the potential to increase the sensitivity and specificity of primary screening (Cox et al, 1995;Ferenczy et al, 1996;Vassilakos et al, 1998). The high negative predictive value of the combined test, close to 100%, may improve cost-effectiveness by permitting screening intervals to be lengthened .…”

mentioning

confidence: 99%

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Primary screening for cervical cancer precursors by the combined use of liquid-based cytology, computer-assisted cytology and HPV DNA testing

et al. 2002

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Primary screening for cervical cancer precursors has considerably evolved with the introduction of new technology to improve the early detection of disease. The objective of this study was to elaborate a diagnostic pathway integrating liquidbased and computer-assisted cytology and human papillomavirus DNA testing to focus screening on women at risk which may be more cost-effective for the healthcare system. A single laboratory analysis was conducted during a 5-month period using liquid-based cytology followed by human papillomavirus DNA testing for women with an abnormal result or with previous abnormal cytology. Human papillomavirus prevalence was estimated by testing 909 consecutive unselected samples. All slides were then rescreened using automated cytologic testing and triaged into a high-or low-score group according to computer results. Of the 8676 slides scanned, 352 had a test result of atypical squamous cells of undetermined significance or worse. Two hundred and ninety-seven (84.3%) samples with an atypical squamous cells of undetermined significance or worse result and 100% of those with detection of high-grade squamous intraepithelial lesions and carcinomas (HSIL+) were triaged into the high-score group. The combination of instrument scores and human papillomavirus results indicated that 51.0% of high score/human papillomavirus-positive cases should be considered as ASCUS+, while 99.6% of low-score/human papillomavirus negative cases remained negative in the final cytologic diagnosis, representing 49.0% of all cases. Of the screened women 89.5% should test negative for human papillomavirus and be reported as such in the final cytologic diagnosis. In conclusion, preliminary results suggest that this diagnostic pathway has the potential to improve primary cervical cancer screening and cost-effectiveness. By using a combination of testing methods to focus screening and clinical attention to cases at risk, it would be possible to lengthen screening intervals for 90% of women and to archive without further review all low-score/human papillomavirus-negative slides, representing 50% of the screening workload.

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Section: Molecular and Cellular Pathologymentioning

confidence: 99%

mentioning

confidence: 99%

Primary screening for cervical cancer precursors by the combined use of liquid-based cytology, computer-assisted cytology and HPV DNA testing

et al. 2002

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“…Cytopathologists have been at the forefront of developing ways of providing genomic information from limited quantities of tissue samples, including using residual cytologic substrates. For years, the residual liquid‐based cytology (LBC) preparation from cervicovaginal gynecological samples has been used for the detection of high‐risk human papillomavirus . The LBC media not only provides optimal preservation of cellular morphology for a cytologic diagnosis, but also preserves nucleic acids for downstream DNA‐/RNA‐based assays.…”

mentioning

confidence: 99%

“…For years, the residual liquid-based cytology (LBC) preparation from cervicovaginal gynecological samples has been used for the detection of high-risk human papillomavirus. 6 The LBC media not only provides optimal preservation of cellular morphology for a cytologic diagnosis, but also preserves nucleic acids for downstream DNA-/RNAbased assays.Recent studies have explored the usefulness of the molecular testing of residual LBC from nongynecological FNA samples, with promising results. Several studies have been published to date describing the usefulness of extracting nucleic acids from residual LBC solution from thyroid FNA specimens for mutation analysis using high-resolution melting polymerase chain reaction (PCR), pyrosequencing, and nextgeneration sequencing (NGS), and evaluating gene rearrangements using quantitative PCR.…”

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confidence: 99%

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Molecular testing of residual cytology samples: Rethink, reclaim, repurpose

Roy‐Chowdhuri

2018

Cancer Cytopathology

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Pap test in a high‐risk population comparison of conventional and liquid‐base cytology

Utagawa

Pereira

Makabe

et al. 2004

Diagnostic Cytopathology

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Liquid-based cytology (LBC) is believed to have better sensitivity than conventional smears (CSs) and offers the possibility to perform molecular assay. The goal of this work was to study the performance of CS and LBC in a high-risk population and to compare the results with the hybrid capture (HC) II for high-risk human papillomavirus (HPV). Samples were collected from selected women with clinical suspicions of low genital tract lesion at Pérola Biygnton Hospital (São Paulo, Brazil). After CS preparation, the brush was introduced in the endocervix and a new sample was collected and rinsed in the preservative medium of the system. The residual material was used to HC2. From 925 cases, LBC was unsatisfactory in 4 (1.51%) cases and CS was unsatisfactory in 100 cases (10.81%); among theses cases HC2(+) reactions was observed in 54 (54%) CSs and 3 (21.4%) LBCs. Considering cases with atypia of undetermined significance (squamous and glandular), 85 (77.27%) cases from LBC and 44 (86.4%) from CS were positive for HC2 assay for high-risk DNA-HPV. The difference among the methods was not significant (P < 0.38). The diagnosis improvement of LBC in comparison with CS was 86% in satisfactory samples, 92.76% in undetermined atypical lesions (including glandular), 83% in positive low-grade squamous intraepithelial lesions (LSIL(+)), and 86.84% in high-grade SIL(+) (HSIL(+)). HC2(+) reactions were observed in 144 CS cases and 266 LBC cases with abnormalities. Our results have showed that LBC was superior to CS in a high-risk population to detect lesions with high concordance with HC2(+) reactions; CSs also exhibit a high concordance with HC2 assay but with inferior performance to detect lesions.

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Human papillomavirus (HPV) DNA assay as an adjunct to liquid‐based Pap test in the diagnostic triage of women with an abnormal Pap smear

Cited by 15 publications

References 14 publications

Primary screening for cervical cancer precursors by the combined use of liquid-based cytology, computer-assisted cytology and HPV DNA testing

Primary screening for cervical cancer precursors by the combined use of liquid-based cytology, computer-assisted cytology and HPV DNA testing

Molecular testing of residual cytology samples: Rethink, reclaim, repurpose

Pap test in a high‐risk population comparison of conventional and liquid‐base cytology

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