Human papillomavirus Type 2 DNA in oral verrucous carcinoma

Abstract

Tissues from patients with oral verrucous carcinoma were examined for the presence of human papillomavirus (HPV). The tissues were stained for the presence of the type common papillomavirus antigen by immunohistochemical staining and the presence of HPV DNA was determined by in situ hybridization with biotin‐labelled HPV DNA probes. Seventeen tissue specimens were obtained from 9 patients, and included pre‐malignant lesions and primary and recurrent tumors. One pre‐malignant lesion was positive for papillomavirus structural antigen. This lesion and lesions from 2 other patients hybridized at low stringency (Tm‐35°) to 3 different HPV probes. By hybridization under high stringency conditions (Tm‐20°), the virus in each case was identified as being HPV2.

Human Papillomavirus DNA in Biopsies of Oral Tissues

“…Oral cancer biopsies have also been shown, in some cases, to contain HPV sequences, de Villiers et al (1985) and Adler-Storthz et al (1986b) detected HPV-2 DNA in oral squamous and verrucous carcinomas, and DNAs of HPV-16 (de Villiers et al, 1985;Maitland et al, 1987) and HPV-18 (Syrjanen et al, 1988) have been reported in other oral squamous celt carcinomas. Additionally, Maitland et al (1987Maitland et al ( , 1989 found that a large proportion of normal oral mucosal samples, as well as carcinomas, exclusively contain HPV-16 sequences, which in most cases varied from the prototype virus in their PstI restriction pattern.…”

Epidemiology: A Tool for the Study of Human Papillomavirus-Related Carcinogenesis

“…Each site has premalignant lesions which progress from mild dysplasia to carcinoma in situ and invasive carcinoma. Reports regarding the presence of HPV genotypes within oral tumors by molecular hybridi zation techniques do appear in the literature [59][60][61]; however, the findings are not conclusive.…”

Comprehensive analysis of human papillomavirus prevalence and the potential role of low-risk types in verrucous carcinoma

“…30 This system allows the typing of both high-risk HPV (types 16,18,26,30,31,33,35,39,45, 51, 52, 56, 58, 59, 66, 68, 70,73, 82i and 82m) and low-risk HPV (6,11,34,40,42,43,44,54,55,57,61,64,71,72,81 and CP6108).…”

“…42 For verrucous carcinoma arising from the head and neck region, it has been reported that high-risk HPV are opportunistic viruses enhancing other carcinogenic factors such as tobacco or alcohol. 43,44 In case of low-risk HPV types, sequence variations increasing their transforming potential has been described. 26 Moreover, it has also been suggested that malignant transformation may be more related to the host response to the HPV infections than to the virus itself.…”