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Identification of human papillomavirus types in oral verruca vulgaris. J Oral Pathol 1986: 15: 230-233.Abstract -Eleven oral verruca vulgaris specimens were examined for the presence of papillomavirus structural antigens by reaction with antibody to type-common antigens and deteetion by the avidin-biotin-peroxidase complex method. The speeimens were also examined by iti situ hybridization with biotinlabelled human papiliomavirus (HPV) DNA to determine the speeific HPV types present in the lesions. Six of the 11 specimens were positive for papillomavirus struetural antigens. Of these 6, 5 hybridized to the HPV Type 2 (HPV2) probe and one to the HPV4 probe.

Tissues from patients with oral verrucous carcinoma were examined for the presence of human papillomavirus (HPV). The tissues were stained for the presence of the type common papillomavirus antigen by immunohistochemical staining and the presence of HPV DNA was determined by in situ hybridization with biotin‐labelled HPV DNA probes. Seventeen tissue specimens were obtained from 9 patients, and included pre‐malignant lesions and primary and recurrent tumors. One pre‐malignant lesion was positive for papillomavirus structural antigen. This lesion and lesions from 2 other patients hybridized at low stringency (Tm‐35°) to 3 different HPV probes. By hybridization under high stringency conditions (Tm‐20°), the virus in each case was identified as being HPV2.

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