Human periodontal ligament stem cells on calcium phosphate scaffold delivering platelet lysate to enhance bone regeneration

Zhao, Zeqing; Jin, Liu; Weir, Michael D.; Zhang, Ning; Zhang, Li; Xie, Xianju; Zhang, Charles; Zhang, Ke; Bai, Yuxing; Xu, Hockin H.K.

doi:10.1039/c9ra08336g

Cited by 12 publications

(28 citation statements)

References 57 publications

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“…The relatively lower expression in nanofiltered growth media could possibly be linked in part to a decrease of FGF content because this growth factor plays a key role in osteogenesis by activating RUNX2, which regulates bone formation and growth, and participates in the anabolic function of osteoblasts [61,62]. Gene expression data obtained here are consistent with previous reports on the stronger stimulation of chondrogenic and osteogenic differentiation using human platelet lysates than FBS [22,30,63].…”

Section: Discussionsupporting

confidence: 89%

Nanofiltration of growth media supplemented with human platelet lysates for pathogen-safe xeno-free expansion of mesenchymal stromal cells

et al. 2020

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Background aims: Human platelet lysate can replace fetal bovine serum (FBS) for xeno-free ex vivo expansion of mesenchymal stromal cells (MSCs), but pooling of platelet concentrates (PCs) increases risks of pathogen transmission. We evaluated the feasibility of performing nanofiltration of platelet lysates and determined the impact on expansion of bone marrowÀderived MSCs. Methods: Platelet lysates were prepared by freeze-thawing of pathogen-reduced (Intercept) PCs suspended in 65% storage solution (SPP+) and 35% plasma, and by serum-conversion of PCs suspended in 100% plasma. Lysates were added to the MSC growth media at 10% (v/v), filtered and subjected to cascade nanofiltration on 35-and 19-nm Planova filters. Media supplemented with 10% starting platelet lysates or FBS were used as the controls. Impacts of nanofiltration on the growth media composition, removal of platelet extracellular vesicles (PEVs) and MSC expansion were evaluated. Results: Nanofiltration did not detrimentally affect contents of total protein and growth factors or the biochemical composition. The clearance factor of PEVs was >3 log values. Expansion, proliferation, membrane markers, differentiation potential and immunosuppressive properties of cells in nanofiltered media were consistently better than those expanded in FBS-supplemented media. Compared with FBS, chondrogenesis and osteogenesis genes were expressed more in nanofiltered media, and there were fewer senescent cells over six passages. Conclusions: Nanofiltration of growth media supplemented with two types of platelet lysates, including one prepared from pathogen-reduced PCs, is technically feasible. These data support the possibility of developing pathogen-reduced xeno-free growth media for clinical-grade propagation of human cells.

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Section: Discussionsupporting

confidence: 89%

Nanofiltration of growth media supplemented with human platelet lysates for pathogen-safe xeno-free expansion of mesenchymal stromal cells

et al. 2020

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“…Metformin (a small molecular glucose lowering drug) and platelet lysate (HPL) carried by CPC-chitosan scaffold can be sustainably released for 15-21 days (Qin et al, 2018;Qiu et al, 2021). Metformin and HPL released by CPC increased the osteogenesis of PDLSCs seeded on CPC scaffolds (Qin et al, 2018;Zhao et al, 2019c).…”

Section: Delivery Of Pdlscs With Various Types Of Scaffoldsmentioning

confidence: 98%

“…Chitosan can be used as a viscous binder to rendered CPC fast-setting and increase the load-bearing capability (Wang et al, 2016). CPC scaffold containing chitosan is an effective delivery vehicle for drugs and proteins (Zhao et al, 2019b;Zhao et al, 2019c). Qin et al (2018) and Qiu et al (2021) revealed that CPC-chitosan scaffolds can sustainably release small molecular drug and growth factors.…”

Section: Delivery Of Pdlscs With Various Types Of Scaffoldsmentioning

confidence: 99%

Periodontal ligament stem cell-based bioactive constructs for bone tissue engineering

Zhao

Jin

Weir

et al. 2022

Front. Bioeng. Biotechnol.

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Objectives: Stem cell-based tissue engineering approaches are promising for bone repair and regeneration. Periodontal ligament stem cells (PDLSCs) are a promising cell source for tissue engineering, especially for maxillofacial bone and periodontal regeneration. Many studies have shown potent results via PDLSCs in bone regeneration. In this review, we describe recent cutting-edge researches on PDLSC-based bone regeneration and periodontal tissue regeneration.Data and sources: An extensive search of the literature for papers related to PDLSCs-based bioactive constructs for bone tissue engineering was made on the databases of PubMed, Medline and Google Scholar. The papers were selected by three independent calibrated reviewers.Results: Multiple types of materials and scaffolds have been combined with PDLSCs, involving xeno genic bone graft, calcium phosphate materials and polymers. These PDLSC-based constructs exhibit the potential for bone and periodontal tissue regeneration. In addition, various osteo inductive agents and strategies have been applied with PDLSCs, including drugs, biologics, gene therapy, physical stimulation, scaffold modification, cell sheets and co-culture.Conclusoin: This review article demonstrates the great potential of PDLSCs-based bioactive constructs as a promising approach for bone and periodontal tissue regeneration.

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“…Besides the development of 3D hierarchical scaffolds, the use of biologicals is currently under investigation to enhance the scaffold performance without scar formation. Recently, various biologics combined with scaffolds have been considered and evaluated in preclinical animal studies to augment tendon repair [ 114 , 115 , 116 ]. The main goal is to achieve a complete functional recovery of the tendon injuries [ 10 , 117 ].…”

Section: Biological Augmentation For Tendon Healingmentioning

confidence: 99%

Innovative Strategies in Tendon Tissue Engineering

et al. 2021

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The tendon is a highly aligned connective tissue that transmits force from muscle to bone. Each year, more than 32 million tendon injuries have been reported, in fact, tendinopathies represent at least 50% of all sports injuries, and their incidence rates have increased in recent decades due to the aging population. Current clinical grafts used in tendon treatment are subject to several restrictions and there is a significant demand for alternative engineered tissue. For this reason, innovative strategies need to be explored. Tendon replacement and regeneration are complex since scaffolds need to guarantee an adequate hierarchical structured morphology and mechanical properties to stand the load. Moreover, to guide cell proliferation and growth, scaffolds should provide a fibrous network that mimics the collagen arrangement of the extracellular matrix in the tendons. This review focuses on tendon repair and regeneration. Particular attention has been devoted to the innovative approaches in tissue engineering. Advanced manufacturing techniques, such as electrospinning, soft lithography, and three-dimensional (3D) printing, have been described. Furthermore, biological augmentation has been considered, as an emerging strategy with great therapeutic potential.

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Human periodontal ligament stem cells on calcium phosphate scaffold delivering platelet lysate to enhance bone regeneration

Abstract: Human periodontal ligament stem cells (hPDLSCs) are promising for tissue engineering applications but have received relatively little attention.

Cited by 12 publications

References 57 publications

Nanofiltration of growth media supplemented with human platelet lysates for pathogen-safe xeno-free expansion of mesenchymal stromal cells

Nanofiltration of growth media supplemented with human platelet lysates for pathogen-safe xeno-free expansion of mesenchymal stromal cells

Periodontal ligament stem cell-based bioactive constructs for bone tissue engineering

Innovative Strategies in Tendon Tissue Engineering

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