Human peritoneal macrophages synthesize leukotrienes B4 and C4

Du, Julie T.; Foegh, Marie L.; Maddox, Yvonne T.; Ramwell, Peter W.

doi:10.1016/0005-2760(83)90003-6

Cited by 47 publications

(5 citation statements)

References 16 publications

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“…So far, the data on leukotriene synthesis in human synovial cells are obscure, but other types of human macrophages, e.g. peritoneal and alveolar macrophages, are able to produce leukotrienes [33][34][35][36]. The basal production of LTB 4 in a primary culture of adherent rheumatic synovial cells was very low as compared to PGE 2 synthesis.…”

Section: Discussionmentioning

confidence: 98%

Eicosanoid production in rheumatoid synovitis

Moilanen

Alanko

Nissilä³

et al. 1989

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Leukotriene B4 (LTB4) synthesis in rheumatoid synovitis was studied using peripheral and synovial fluid polymorphonuclear leukocytes (PMNs) and rheumatic synovial lining cells. No differences were found in LTB4 synthesis between peripheral PMNs from healthy volunteers and rheumatoid arthritis patients. When peripheral and synovial PMNs from the same RA patient were compared, arachidonic acid-induced LTB4 synthesis in synovial fluid PMNs was increased 1.7-7.2 fold, whereas the response to Ca ionophore A23187 stimulation was similar. This suggests 5-lipoxygenase stimulating factor(s) in inflamed joints. Rheumatic synovial lining cells in a primary cell culture produced small amounts of LTB4, the concentrations being less than 0.1 per cent of those of prostaglandin E2 (PGE2). PGE2 synthesis in synovial cells was increased when arachidonic acid or interleukin-1 was added to the culture, whereas LTB4 production remained unaltered. The present results suggest that in inflamed joints LTB4 originates mainly from PMNs whereas synovial lining cells are the source for PGE2.

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Section: Discussionmentioning

confidence: 98%

Eicosanoid production in rheumatoid synovitis

Moilanen

Alanko

Nissilä³

et al. 1989

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“…and itidoinethacin inhibits synthesis of alt three products measured. This purified macrophage preparation is useful for various purposes and has been used recently to study the action of mitamycin C [12] and the synthesis of leukotrienes B and C [8].…”

Section: Discussionmentioning

confidence: 99%

A Routine Source of Human Peritoneal Macrophages

et al. 1984

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The routine availability of nucleated human cells for experimental use in limited in the absence of venipuncture. In this paper we have demonstrated that macrophages may be harvested routinely from the waste dialysis bags of patients undergoing continuous ambulatory peritoneal dialysis. These cells were identified as macrophages by morphology, adherence, phagocytosis, chemotaxis, non-specific esterase staining and peroxidase staining. Macrophages from patients with end-stage renal disease produced arachidonate cyclo-oxygenase products in a pattern similar to that of ascites macrophages obtained from patients with normal kidney function. Arachidonate metabolism was shown to be manipulatable. Thus, indomethacin blocked synthesis of cyclooxygenase products, and OKY-1581, a specific thromboxane synthase inhibitor, increased the release of prostaglandin E2 and prostacyclin, measured as its stable breakdown product 6-keto-prostaglandin F1, whereas the thromboxane B2 synthesis was effectively inhibited.

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“…LTB4, an arachidonic acid derivative, acts via the two GPCR LTB4Rs, LTB4R1 (BLT1) and LTB4R2 (BLT2) [120,121], to stimulate macrophage, neutrophil, eosinophil, and mast cell chemotaxis [122,123]. Mast cells are a major source of LTB4 [124], but it is also secreted by human alveolar macrophages [125] and murine peritoneal macrophages [126], suggesting the existence of an autocrine pathway in this lineage. The importance of LTB4-mediated cellular recruitment is highlighted by the failure of inflammatory cells to infiltrate into joints and initiate CIA in LTB4RϪ/Ϫ mice [127].…”

Section: The Leukotriene B4 Receptor (Ltb4r)mentioning

confidence: 99%

G-protein-coupled receptor expression, function, and signaling in macrophages

Lattin

Zidar

Schroder

et al. 2007

Journal of Leukocyte Biology

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G-protein-coupled receptors (GPCRs) are widely targeted in drug discovery. As macrophages are key cellular mediators of acute and chronic inflammation, we review here the role of GPCRs in regulating macrophage function, with a focus on contribution to disease pathology and potential therapeutic applications. Within this analysis, we highlight novel GPCRs with a macrophage-restricted expression profile, which provide avenues for further exploration. We also review an emerging literature, which documents novel roles for GPCR signaling components in GPCR-independent signaling in macrophages. In particular, we examine the crosstalk between GPCR and TLR signaling pathways and highlight GPCR signaling molecules which are likely to have uncharacterized functions in this cell lineage.

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Human peritoneal macrophages synthesize leukotrienes B4 and C4

Cited by 47 publications

References 16 publications

Eicosanoid production in rheumatoid synovitis

Eicosanoid production in rheumatoid synovitis

A Routine Source of Human Peritoneal Macrophages

G-protein-coupled receptor expression, function, and signaling in macrophages

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