Human placenta-derived mesenchymal progenitor cells support culture expansion of long-term culture-initiating cells from cord blood CD34+ cells

Superior ex vivo cord blood expansion following co-culture with bone marrow-derived mesenchymal stem cells

“…[48][49][50][51][52][53] One component of the hematopoietic microenvironment, mesenchymal stem cells (MSCs), can be isolated as plastic adherent cells from a variety of fetal and adult tissues. [54][55][56][57] In addition to providing an alternative (co-culture) strategy for CB expansion, allogeneic MSC coadministration has been shown to promote engraftment of human CD34 þ cells in NOD/SCID mice and fetal sheep, [57][58][59][60][61] and MSCs have been shown to have clinical immunomodulatory activity that may impact on graft-versus-host disease in transplant patients. 59,[62][63][64][65][66][67][68][69] CB-MSC co-culture does not require the isolation of CD34 þ or CD133 þ cells before expansion, minimizing manipulation and loss of HPC.…”

Mesenchymal stem cells derived from human placenta suppress allogeneic umbilical cord blood lymphocyte proliferation

“…Placenta and UCB are homogeneous (both are fetal appertaining and extraembryonic mesoderm original), and the adherent cells from placenta as feeder is much better than the stromal cells from BM for UCB expanded in vitro [20]. Our study aimed at isolating and characterizing MSC in human placenta, which would possibly open a new and rich source of MSC for experimental and clinical needs, especial for the transplantation of UCB HSC.…”

Isolation and Characteristics of Mesenchymal Stromal Cells from Different Parts of Placenta

“…Several protocols described two different methods of isolation: mechanical separation and enzyme digestion [9,10]. In our experiment, we isolated MSCs by mechanical cut and collagenase digestion to compare cell populations from a given tissue isolated with different methods.…”

“…Others indicated that a population of fetal cells can be isolated without maternal contamination [9,10,22]. The possibility of isolation of a pure population of maternal or fetal MSCs depends on a wide variety of factors, such as the mother's diseases, etc.…”