Human platelet lysate supportsex vivoexpansion and enhances osteogenic differentiation of human bone marrow-derived mesenchymal stem cells

Abstract

MSCs (mesenchymal stem cells) with their versatile growth and differentiation potential are ideal candidates for use in regenerative medicine and are currently making their way into clinical trials, which requires the development of xeno-free protocols for their culture. In this study, MSCs were cultured in 10% FCS or 7.5% HPL (human platelet lysate)-supplemented media. We found that both groups of MSCs showed a comparable morphology, phenotype and proliferation. The percentage of cells in the S- and G2-/M-phases, however, was slightly up-regulated (P<0.01) in HPL group. HPL contains PDGF (platelet derived growth factor)-AB and IGF (insulin-like growth factor)-1. In addition, compared with FCS group, MSCs in HPL group showed an increase in osteogenic differentiation and a decrease in adipogenic differentiation. In conclusion, MSCs in HPL-supplemented media maintained similar growing potential and phenotype, while osteogenic potential was enhanced. HPL offers a promising alternative to FCS for MSC expansion for clinical application, especially in bone injury diseases.

Venous Blood Derivatives as FBS-Substitutes for Mesenchymal Stem Cells: A Systematic Scoping Review

“…Platelets lyse was triggered by either, freeze-thaw cycles (1 to 5), ultrasound (63) or chemical treatment (43). HPL presented a high growth factors and cytokines content (74)(75)(76)(77). HPL seemed to be better than FBS for ex vivo MSC expansion (52,72,78,79) since HPL-expanded MSC did not present telomerase shortening (68).…”

Evaluation of intra-articular injection of autologous platelet lysate (PL) in horses with osteoarthritis of the distal interphalangeal joint

“…PL is considered a perfect candidate for the management of osteoarthritis, mainly due to its ability to promote ex vivo expansion of mesenchymal stem cells (MSCs) used for bone and cartilage engineering (Zaky et al 2008;Chevallier et al 2010;Xia et al 2011;Sofat & Kuttapitiya 2014;Tan et al 2015). MSCs are found in multiple tissues including synovial joints and have shown the potential to differentiate into cells of several tissues including bone and cartilage (Sofat & Kuttapitiya 2014).…”

Partnership between platelet-rich plasma and mesenchymal stem cells: in vitro experience

“…When those steps are performed, they proceed to filtration through 0.22 um filters and addition of heparin (2U/ml) to the lysates 35,40,57 . This procedure is the most commonly found in the papers studied, but there are some authors which prefer performing chemical activation instead of thermic/mechanical activation and use of thrombin and/or calcium chloride 7,17,34,41,45,49 .…”

“…Besides, PRP did not interfere with osteogenic differentiation and most authors found similar osteogenic differentiation capacity when using one or the other supplement (PRP versus FBS). Albeit few studies have shown that PRP enhanced MSCs osteogenic differentiation in 2D cultures 14,27,35,40 and in 3D cultures 9,22,43 . Interestingly, Hemeda et al 56 found reduced osteogenic differentiation when MSCs where cultured with PRP with high heparine concentrations which were used to avoid PRP clotting.…”