Human prostasomes express CD48 and interfere with NK cell function

Tarazona, Raquel; Delgado, Elena; Guarnizo, Marta Campos; Roncero, R.G.; Morgado, Sara; Sánchez-Correa, Beatriz; Gordillo, Juan; DeJulián, J.; Casado, Javier G.

doi:10.1016/j.imbio.2010.03.002

Cited by 55 publications

(44 citation statements)

References 38 publications

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“…Membrane vesicles from bovine seminal fluid have been demonstrated to have similar effects on proliferation, phagocytotic activity and superoxide production by leukocytes (Lazarevic et al 1995). Additionally, human prostasomes have been shown to contain CD48, which is the ligand for activating NK cell receptor 2B4 (CD244) (Tarazona et al 2011). NK cell activity decreases after incubation in the presence of prostasomes, suggesting that prostasomes may also play a role in controlling NK cell activity in the female reproductive tract.…”

Section: Prostasomes Interact With Immune Cellsmentioning

confidence: 99%

Prostasomes: extracellular vesicles from the prostate

Aalberts¹,

Stout²,

Stoorvogel³

2014

Reproduction

173

150

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The term 'prostasomes' is generally used to classify the extracellular vesicles (EVs) released into prostatic fluid by prostate epithelial cells. However, other epithelia within the male reproductive tract also release EVs that mix with 'true' prostasomes during semen emission or ejaculation. Prostasomes have been proposed to regulate the timing of sperm cell capacitation and induction of the acrosome reaction, as well as to stimulate sperm motility where all three are prerequisite processes for spermatozoa to attain fertilising capacity. Other proposed functions of prostasomes include interfering with the destruction of spermatozoa by immune cells within the female reproductive tract. On the other hand, it is unclear whether the distinct presumed functions are performed collectively by a single type of prostasome or by separate distinct sub-populations of EVs. Moreover, the exact molecular mechanisms through which prostasomes exert their functions have not been fully resolved. Besides their physiological functions, prostasomes produced by prostate tumour cells have been suggested to support prostate cancer spread development, and prostasomes in peripheral blood plasma may prove to be valuable biomarkers for prostate cancer.

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Section: Prostasomes Interact With Immune Cellsmentioning

confidence: 99%

Prostasomes: extracellular vesicles from the prostate

Aalberts¹,

Stout²,

Stoorvogel³

2014

Reproduction

173

150

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“…In order to compare the susceptibility of hASCs and hBMMSCs to NK cell-mediated lysis, degranulation assays were performed by the detection of CD107a/b surface molecule as previously described [43]. The degranulation assay is a highly sensitive method, and its results are strictly correlated with NK cell cytotoxicity [44].…”

Section: Low Degranulation Activity Of Nk Cells In Response To Hascsmentioning

confidence: 99%

Human Adipose-Derived Stem Cells Impair Natural Killer Cell Function and Exhibit Low Susceptibility to Natural Killer-Mediated Lysis

DelaRosa

Sánchez-Correa

Morgado

et al. 2012

Stem Cells and Development

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Human adipose-derived stem cells (hASCs) have been successfully used in treating numerous diseases. However, several aspects need to be considered, particularly in the context of allogeneic cell therapy. To better understand hASCs-host interactions, we studied the phenotype of hASCs and their modulatory effect on natural killer (NK) cells by using bone marrow-mesenchymal stem cells (hBM-MSCs) as a reference. The hASCs displayed a lower susceptibility to NK cell-mediated lysis and a lower expression of ligands for DNAM-1 when compared with hBM-MSCs. Moreover, here we demonstrated that hASCs and hBM-MSCs can modulate NK cells through the action of soluble factors such as indoleamine 2,3-dioxygenase. Altogether, these results suggest that for an adoptive cell therapy based on the transfer of allogeneic hASCs, the NK-hASCs crosstalk will not result in an immediate recognition of the transferred cells. Thus, hASCs may remain in the tissue long enough to balance the immune response before being cleared.

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“…Interactions between NK cells and purified prostasomes resulted in a decrease of CD244 expression. Moreover, the decreased NK cell activity cultured in the presence of prostasomes, suggests that these vesicles may immune-modulate the local environment within the female reproductive tract, preventing the immune-mediated sperm destruction and prolonging the sperms' survival rate (27). It is also suggested that the immune-suppressive activity of the human SF components protect inseminated sperms in female reproductive tract (28); stimulating precancerous cells to progress to full carcinoma (29).…”

Section: Sf Contains High Concentration (About 10mentioning

confidence: 99%