Human recombinant stem-cell factor induces melanocytic hyperplasia in susceptible patients

Grichnik, James M.; Crawford, Jeffrey; Jiménez, Francisco Javier; Kurtzber, Joanne; Buchanan, Mark D; Blackwell, Susan; Clark, Robert E.; Hitchcock, Michael G.

doi:10.1016/0190-9622(95)91274-6

Cited by 57 publications

(60 citation statements)

References 20 publications

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“…28,29) Previous studies suggested that SCF/ KIT signaling is involved in the mechanism of melanocyte migration, such as melanocyte migration from the neural crest into the epidermis, 30) melanocyte distribution in the hair follicle during fetal development, 31) melanocyte migration in chemotherapy-induced hair loss 32) and increased migration of melanocytes into the epidermis caused by local SCF injection. 33) In vitro, SCF stimulates epidermal melanocyte chemokinesis and increases the speed of melanocyte movement on FN-coated dishes, 21,34) but the effect of SCF on MPs migration was unknown. Thus, our objective was to determine whether SCF and the matrix proteins FN, LN and CIV are chemotactic to MPs and whether SCF has synergistic effects with these matrix proteins.…”

Section: Fig 3 the Regulatory Effect Of Scf On Mps Adhesion To Matrmentioning

confidence: 99%

Stem Cell Factor Combined with Matrix Proteins Regulates the Attachment and Migration of Melanocyte Precursors of Human Hair Follicles <i>in Vitro</i>

Wang

et al. 2013

Biological & Pharmaceutical Bulletin

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In conjunction with matrix proteins, stem cell factor (SCF) plays an important role in the migration of melanocyte precursors (MPs) derived from the mouse embryo. However, no studies have demonstrated an effect of SCF on human follicular MPs migration in vitro. In this report, first we demonstrate the immature state of the follicular MPs. Then cell attachment rate was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method. Standard 48-well chemotaxis chambers were used for a transfilter migration assay. F-actin was labeled by rhodamine-conjugated phalloidin, and then organization of the actin cytoskeleton was observed by confocal microscope. In the results, we directly show that MPs adhere more strongly to fibronectin (FN), laminin (LN) and type IV collagen (CIV) than to the negative control. SCF decreased the adhesion of MPs to FN and CIV. A chemotaxis analysis showed that FN and CIV have chemotactic effects on MPs. FN showed an obvious increase in chemotactic effects on MPs with SCF treatment comparing with the control group, but there were no significant changes in the levels of chemotaxis with CIV and LN when the cells were treated with SCF. SCF was chemotactic to MPs, and the presence of FN caused a statistically significant increase in MPs migration at various concentrations of SCF. Furthermore, we showed that SCF, in combination with FN, could induce an apparent increase in actin stress fiber formation in MPs. Our results indicate that SCF, in combination with matrix proteins and in particular with FN, regulates the movement of MPs by both altering cell attachment and increasing cell chemotaxis.Key words melanocyte precursor; stem cell factor; matrix protein; attachment; chemotaxis When vitiligo patients undergo repigmentation, they develop small pigmented islands that expand and coalesce to form normally pigmented skin.1) It has been demonstrated that these islands of pigmentation are derived from melanocyte precursors (MPs) in the outer root sheath (ORS) of hair follicles. 2)Although this phenomenon has been clinically well supported and MPs have been cultured successfully in vitro, little previous work has been performed to evaluate the migration capability of MPs. [3][4][5][6] When cells migrate, they synchronously attach and detach to various matrix proteins. During the repigmentation of vitiliginous skin, it is thought that MPs become activated, migrate along the basement membrane under the ORS into depigmented skin and then differentiate into mature melanocytes.2,7) The major structural proteins of the basement membrane zone (BMZ) of both normal skin and hair follicles are type IV collagen (CIV), laminin (LN) and entactin. Fibronectin (FN) present in the BMZ located under the cells of the ORS is not a normal epidermal BMZ component. It can only be identified in the epidermal BMZ during embryonic development.8,9) Therefore, there are some differences between epidermal melanocytes and MPs in the matrix proteins surrounding the BMZ. Previous authors have evaluated the impact o...

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Section: Fig 3 the Regulatory Effect Of Scf On Mps Adhesion To Matrmentioning

confidence: 99%

Stem Cell Factor Combined with Matrix Proteins Regulates the Attachment and Migration of Melanocyte Precursors of Human Hair Follicles <i>in Vitro</i>

Wang

et al. 2013

Biological & Pharmaceutical Bulletin

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“…Thus, SCF regulates induction of proliferation, differentiation, survival, integrin expression, migration and melanin pigmentation in melanoblasts (Miura et al, 1992;Lahav et al, 1994;Scott et al, 1994;Luo et al, 1995;Sviderskaya et al, 1995;Costa et al, 1996;Guo et al, 1997). Furthermore, SCF induces an increase of melanocyte numbers after subcutaneous injections (Grichnik et al, 1995;Costa et al, 1996). SCF is produced by diverse cell types, including fibroblasts, keratinocytes and endothelial cells .…”

mentioning

confidence: 99%

Expression of SCF splice variants in human melanocytes and melanoma cell lines: potential prognostic implications

et al. 2000

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Summary Stem cell factor (SCF), the ligand for c-Kit, is known to regulate developmental and functional processes of haematopoietic stem cells, mast cells and melanocytes. Two different splice variants form predominantly soluble (sSCF or SCF-1) and in addition some membranebound SCF (mSCF or SCF-2). In order to explore the prognostic significance of these molecules in melanoma, total SCF, SCF splice variants and c-Kit expression were studied in normal skin melanocytes and in 11 different melanoma cell lines, using reverse transcription polymerase chain reaction, immunocytochemistry and enzyme-linked immunosorbent assay. Nine of the 11 melanoma cell lines expressed SCF-1 mRNA, only two of them SCF-2, and these two also SCF-1. Coexpression of both SCF-1 and c-Kit was noted in five cell lines, and only one cell line as well as normal melanocytes expressed both SCF-1 and SCF-2 as well as c-Kit. Corresponding results were obtained on immunocytochemical staining. Of three exemplary melanoma cell lines studied, two expressing SCF mRNA also released SCF spontaneously and on stimulation, whereas the line lacking SCF and c-kit mRNA (SK-Mel-23) failed to do so. These data demonstrate thus that melanoma cell lines, particularly those known to metastasize in vivo, lose the ability to express SCF-2 mRNA, suggesting that this molecule may serve, next to c-Kit, as a prognostic marker for malignant melanoma.

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“…43 This was not prevented by premedication with ranitidine, cetrizine and salbutamol. Allergic reaction to SCF is postulated to be caused by mast cell stimulation 44,45 and administration of SCF should be done in a supervised setting.…”

Section: Discussionmentioning

confidence: 99%

Optimising parameters for peripheral blood leukapheresis after r-metHuG-CSF (filgrastim) and r-metHuSCF (ancestim) in patients with multiple myeloma: a temporal analysis of CD34+ absolute counts and subsets

Chin‐Yee

Keeney

Stewart

et al. 2002

Bone Marrow Transplant

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Summary:Patients (n = 69) with multiple myeloma undergoing peripheral blood stem cell collection (PBSC) were treated with cyclophosphamide and a combination of recombinant methionyl human granulocyte colony-stimulating factor (r-metHuG-CSF, filgrastim) and recombinant methionyl human stem cell factor (r-metHuSCF, ancestim). The objectives of this study were to determine: (1) The proportion of patients reaching a target yield of у5 ؋ 10 6 CD34 + cells/kg in one or two successive large-volume (20 liter) leukapheresis procedures; (2) the optimal collection time for leukapheresis; (3) mobilization kinetics of CD34 + subsets in response to G-CSF/SCF. All patients were mobilized with cyclophosphamide (2.5 g/m 2 ) on day 0 followed by filgrastim (10 g/kg ) plus ancestim (20 g/kg) commencing day 1 and continuing to day 11 or 12. Of the 65 evaluable patients, 57 were considered not heavily pretreated and 96.5% and early progenitors and the ability to predictably schedule leukapheresis.

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Human recombinant stem-cell factor induces melanocytic hyperplasia in susceptible patients

Cited by 57 publications

References 20 publications

Stem Cell Factor Combined with Matrix Proteins Regulates the Attachment and Migration of Melanocyte Precursors of Human Hair Follicles <i>in Vitro</i>

Stem Cell Factor Combined with Matrix Proteins Regulates the Attachment and Migration of Melanocyte Precursors of Human Hair Follicles <i>in Vitro</i>

Expression of SCF splice variants in human melanocytes and melanoma cell lines: potential prognostic implications

Optimising parameters for peripheral blood leukapheresis after r-metHuG-CSF (filgrastim) and r-metHuSCF (ancestim) in patients with multiple myeloma: a temporal analysis of CD34+ absolute counts and subsets

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