Human Respiratory Syncytial Virus-Induced Immune Signature of Infection Revealed by Transcriptome Analysis of Clinical Pediatric Nasopharyngeal Swab Samples

Lamballerie, Claire Nicolas de; Pizzorno, Andrés; Dubois, Julia; Padey, Blandine; Julien, Thomas; Traversier, Aurélien; Carbonneau, Julie; Orcel, Elody; Lina, Bruno; Hamelin, Marie‐Ève; Roche, Magali; Textoris, Julien; Boivin, Guy; Legras-Lachuer, Catherine; Terrier, B.; Rosa‐Calatrava, Manuel

doi:10.1093/infdis/jiaa468

Cited by 7 publications

(7 citation statements)

References 48 publications

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“…This model, consisting of human airway primary cells grown at the air/liquid interface, is a truly relevant model for studying respiratory viruses and within-host interactions. For example, our group has used this model several times to characterize and compare different respiratory viruses such as influenza viruses, RSV, hMPV [29,34] or [28], but also to experimentally simulate scenarios of interactions between pathogens, including bacterial/fungal superinfections [35][36][37].…”

Section: Discussionmentioning

confidence: 99%

Interactions Between Severe Acute Respiratory Syndrome Coronavirus 2 Replication and Major Respiratory Viruses in Human Nasal Epithelium

Pizzorno

Padey

Dulière

et al. 2022

The Journal of Infectious Diseases

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The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), along with extensive non-pharmacological interventions, have profoundly altered the epidemiology of major respiratory viruses. Some studies have described virus-virus interactions, particularly manifested by viral interference mechanisms at different scales. Still, our knowledge of the mutual interactions between SARS-CoV-2 and other respiratory viruses remains incomplete. Here, we studied the interactions between SARS-CoV-2 and several respiratory viruses (influenza, RSV, hMPV, and hRV) in a reconstituted human epithelial airway model, exploring different scenarios affecting the sequence and timing of co-infections. We show that the virus type and the sequence of infections are key parameters of virus-virus interactions, having the impact of primary infections on the regulation of the immune response a determinant role in the outcome of secondary infections.

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Section: Discussionmentioning

confidence: 99%

Interactions Between Severe Acute Respiratory Syndrome Coronavirus 2 Replication and Major Respiratory Viruses in Human Nasal Epithelium

Pizzorno

Padey

Dulière

et al. 2022

The Journal of Infectious Diseases

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“…Similar to many immune receptors, the gene promoter region contains nuclear factor kappa-light-chain enhancer of activated B cell and nuclear factor of activated T-cell binding motifs [23]. Previous research has highlighted the importance of MCEMP1 in sepsis, viral infection [24][25][26], and a potential useful prognostic tool in stroke patients [26]. Interleukin-1 receptor-associated kinase 3 (IRAK3) is a negative regulator for toll-like receptor (TLR) signaling pathways in innate and adaptive immune responses [27].…”

Section: Discussionmentioning

confidence: 99%

Crucial Genes in Aortic Dissection Identified by Weighted Gene Coexpression Network Analysis

Zhang

Chen

Lin

et al. 2022

Journal of Immunology Research

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Background. Aortic dissection (AD) is a lethal vascular disease with high mortality and morbidity. Though AD clinical pathology is well understood, its molecular mechanisms remain unclear. Specifically, gene expression profiling helps illustrate the potential mechanism of aortic dissection in terms of gene regulation and its modification by risk factors. This study was aimed at identifying the genes and molecular mechanisms in aortic dissection through bioinformatics analysis. Method. Nine patients with AD and 10 healthy controls were enrolled. The gene expression in peripheral mononuclear cells was profiled through next-generation RNA sequencing. Analyses including differential expressed gene (DEG) via DEGseq, weighted gene coexpression network (WGCNA), and VisANT were performed to identify crucial genes associated with AD. The Database for Annotation, Visualization, and Integrated Discovery (DAVID) was also utilized to analyze Gene Ontology (GO). Results. DEG analysis revealed that 1,113 genes were associated with AD. Of these, 812 genes were markedly reduced, whereas 301 genes were highly expressed, in AD patients. DEGs were rich in certain categories such as MHC class II receptor activity, MHC class II protein complex, and immune response genes. Gene coexpression networks via WGCNA identified 3 gene hub modules, with one positively and 2 negatively correlated with AD, respectively. Specifically, module 37 was the most strongly positively correlated with AD with a correlation coefficient of 0.72. Within module 37, five hub genes (AGFG1, MCEMP1, IRAK3, KCNE1, and CLEC4D) displayed high connectivity and may have clinical significance in the pathogenesis of AD. Conclusion. Our analysis provides the possible association of specific genes and gene modules for the involvement of the immune system in aortic dissection. AGFG1, MCEMP1, IRAK3, KCNE1, and CLEC4D in module M37 were highly connected and strongly linked with AD, suggesting that these genes may help understand the pathogenesis of aortic dissection.

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“…Recent findings on the molecular basis of neuroimmune responses revealed that the transcriptomic results are in line with a few previously reported studies of respiratory viral infections like Influenza A virus, RV, and RSV. Moreover, these data highlighted putative biomarkers of interest as a direct reflection of each virus infection and deserve further investigation for the evaluation/prediction of future innovative treatments [ 64 , 65 , 66 ].…”

Section: Viruses Impacting Respiratory Systemmentioning

confidence: 99%

SARS-CoV-2 and Other Respiratory Viruses in Human Olfactory Pathophysiology

Wade,

Diouara,

Ngom

et al. 2024

Microorganisms

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Acute respiratory viruses (ARVs) are the leading cause of diseases in humans worldwide. High-risk individuals, including children and the elderly, could potentially develop severe illnesses that could result in hospitalization or death in the worst case. The most common ARVs are the Human respiratory syncytial virus, Human Metapneumovirus, Human Parainfluenza Virus, rhinovirus, coronaviruses (including SARS and MERS CoV), adenoviruses, Human Bocavirus, enterovirus (-D68 and 71), and influenza viruses. The olfactory deficits due to ARV infection are a common symptom among patients. This review provides an overview of the role of SARS-CoV-2 and other common ARVs in the development of human olfactory pathophysiology. We highlight the critical need to understand the signaling underlying the olfactory dysfunction and the development of therapeutics for this wide-ranging category of AVRs to restore the altered or loss of smell in affected patients.

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Human Respiratory Syncytial Virus-Induced Immune Signature of Infection Revealed by Transcriptome Analysis of Clinical Pediatric Nasopharyngeal Swab Samples

Cited by 7 publications

References 48 publications

Interactions Between Severe Acute Respiratory Syndrome Coronavirus 2 Replication and Major Respiratory Viruses in Human Nasal Epithelium

Interactions Between Severe Acute Respiratory Syndrome Coronavirus 2 Replication and Major Respiratory Viruses in Human Nasal Epithelium

Crucial Genes in Aortic Dissection Identified by Weighted Gene Coexpression Network Analysis

SARS-CoV-2 and Other Respiratory Viruses in Human Olfactory Pathophysiology

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