Human RNAi pathway: crosstalk with organelles and cells

Jamalkandi, Sadegh Azimzadeh; Azadian, Esmaeel; Masoudi‐Nejad, Ali

doi:10.1007/s10142-013-0344-1

Cited by 17 publications

(10 citation statements)

References 196 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mature miRs are passed on to the RNA induced silencing complex (RISC) containing the argonaute protein with RNase activity. One strand is selected as a guide, which pairs with the target to facilitate target translation inhibition or degradation (reviewed in [5]).…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Hepatitis B Virus Replication by Helper Dependent Adenoviral Vectors Expressing Artificial Anti-HBV Pri-miRs from a Liver-Specific Promoter

Mowa

Crowther

Ely

et al. 2014

BioMed Research International

View full text Add to dashboard Cite

Research on applying RNA interference (RNAi) to counter HBV replication has led to identification of potential therapeutic sequences. However, before clinical application liver-specific expression and efficient delivery of these sequences remain an important objective. We recently reported short-term inhibition of HBV replication in vivo by using helper dependent adenoviral vectors (HD Ads) expressing anti-HBV sequences from a constitutively active cytomegalovirus (CMV) promoter. To develop the use of liver-specific transcription regulatory elements we investigated the utility of the murine transthyretin (MTTR) promoter for expression of anti-HBV primary microRNAs (pri-miRs). HD Ads containing MTTR promoter effected superior expression of anti-HBV pri-miRs in mice compared to HD Ads containing the CMV promoter. MTTR-containing HD Ads resulted in HBV replication knockdown of up to 94% in mice. HD Ads expressing trimeric anti-HBV pri-miRs silenced HBV replication for 5 weeks. We previously showed that the product of the codelivered lacZ gene induces an immune response, and the duration of HBV silencing in vivo is likely to be attenuated by this effect. Nevertheless, expression of anti-HBV pri-miRs from MTTR promoter is well suited to countering HBV replication and development of HD Ads through attenuation of their immunostimulatory effects should advance their clinical utility.

show abstract

Section: Introductionmentioning

confidence: 99%

Inhibition of Hepatitis B Virus Replication by Helper Dependent Adenoviral Vectors Expressing Artificial Anti-HBV Pri-miRs from a Liver-Specific Promoter

Mowa

Crowther

Ely

et al. 2014

BioMed Research International

View full text Add to dashboard Cite

show abstract

“…Yet another revision is the inclusion of miRNA molecules in the model as a representative of all RNA species which negatively affect protein production by regulating mRNA half-life. Regulatory effects of miRNAs on target mRNAs are not exclusively at the post-transcriptional level (miRNA-mRNA) and also show their effects in the form of miRNA-DNA interactions [ 9 – 11 , 20 ]. The definition of DNA and Activator has also changed after the emergence of epigenetics and discovery of the post-translational modification phenomenon.…”

Section: Methodsmentioning

confidence: 99%

“…Moreover, genes are transcribed in the form of distinct splice variants, where exons are omitted or alternative combinations of exons are utilized, leading to a diverse set of gene products with functional differences. The latest species discovered in the field is microRNA (miRNA) which has led to further elucidation of the regulatory process of transcription in which information flow is blocked either by degrading the transcribed mRNA or by facilitating gene silencing at the DNA level [ 8 – 11 ] ( Fig 1A ).…”

Section: Introductionmentioning

confidence: 99%

A logic-based dynamic modeling approach to explicate the evolution of the central dogma of molecular biology

et al. 2017

View full text Add to dashboard Cite

It is nearly half a century past the age of the introduction of the Central Dogma (CD) of molecular biology. This biological axiom has been developed and currently appears to be all the more complex. In this study, we modified CD by adding further species to the CD information flow and mathematically expressed CD within a dynamic framework by using Boolean network based on its present-day and 1965 editions. We show that the enhancement of the Dogma not only now entails a higher level of complexity, but it also shows a higher level of robustness, thus far more consistent with the nature of biological systems. Using this mathematical modeling approach, we put forward a logic-based expression of our conceptual view of molecular biology. Finally, we show that such biological concepts can be converted into dynamic mathematical models using a logic-based approach and thus may be useful as a framework for improving static conceptual models in biology.

show abstract

“…Small non-coding RNAs regulate the expression of protein-coding genes through sequence-specific recognition, binding to 3′ or 5′-untranslated region of target mRNA or promoter sequences, thus controlling mRNA levels by post-transcriptional mechanisms [29, 30]. Moreover, the role of these molecules in specific cellular or physiological processes can be investigated through the deletion or inhibition of microRNA (miRNA) processing machinery [6, 31]. In addition, RNAs are secreted from cells in the bloodstream and directing themselves to the targeted cells.…”

Section: Genomics and Transcriptomicsmentioning

confidence: 99%

Multi-Omics Approach: New Potential Key Mechanisms Implicated in Cardiorenal Syndromes

Virzì¹,

Clementi²,

Battaglia³

et al. 2019

Cardiorenal Med

View full text Add to dashboard Cite

Cardiorenal syndromes (CRS) include a scenario of clinical interactions characterized by the heart and kidney dysfunction. The crosstalk between cardiac and renal systems is clearly evidenced but not completely understood. Multi-factorial mechanisms leading to CRS do not involve only hemodynamic parameters. In fact, in recent works on organ crosstalk endothelial injury, the alteration of normal immunologic balance, cell death, inflammatory cascades, cell adhesion molecules, cytokine and chemokine overexpression, neutrophil migration, leukocyte trafficking, caspase-mediated induction of apoptotic mechanisms and oxidative stress has been demonstrated to induce distant organ dysfunction. Furthermore, new alternative mechanisms using the multi-omics approach may be implicated in the pathogenesis of cardiorenal crosstalk. The study of “omics” modifications in the setting of cardiovascular and renal disease represents an emerging area of research. Over the last years, indeed, many studies have elucidated the exact mechanisms involved in gene expression and regulation, cellular communication and organ crosstalk. In this review, we analyze epigenetics, gene expression, small non-coding RNAs, extracellular vesicles, proteomics, and metabolomics in the setting of CRS.

show abstract

Human RNAi pathway: crosstalk with organelles and cells

Cited by 17 publications

References 196 publications

Inhibition of Hepatitis B Virus Replication by Helper Dependent Adenoviral Vectors Expressing Artificial Anti-HBV Pri-miRs from a Liver-Specific Promoter

Inhibition of Hepatitis B Virus Replication by Helper Dependent Adenoviral Vectors Expressing Artificial Anti-HBV Pri-miRs from a Liver-Specific Promoter

A logic-based dynamic modeling approach to explicate the evolution of the central dogma of molecular biology

Multi-Omics Approach: New Potential Key Mechanisms Implicated in Cardiorenal Syndromes

Contact Info

Product

Resources

About