Human Root Caries: Histopathology of Arrested Lesions

Schüpbach, P.; Lutz, F; Guggenheim, B.

doi:10.1159/000261436

Cited by 68 publications

(47 citation statements)

References 22 publications

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“…These include a hypermineralised surface layer [Hals and Selvig, 1977], irritation dentine on the pulpal wall [Schüpbach et al, 1990a] and dentine sclerosis beneath a lesion [Schüpbach et al, 1992]. However, enamel 'undermining' at the CEJ noted by Schüpbach et al [1992] was also seen in our study ( fig. 2B).…”

Section: Discussionsupporting

confidence: 53%

Fluoride Distribution in Sound and Carious Root Tissues of Human Teeth

Shu

Pearce²,

Sissons³

et al. 1998

Caries Res

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Proton probe analysis has been used to provide for the first time quantitative F concentration data in carious root tissues from subjects consuming water containing 1 ppm F. In small lesions at the neck of the tooth with minimal tissue loss the F concentration was significantly higher at the outer lesion edge than at the outer edge of adjacent sound root tissue. In one sample with high F values the lesion edge had 19,000 ppm F and the adjacent sound root surface 5,400 ppm F (μg F/g apatite). In large lesions with extensive cavitation F was again concentrated in the outer edge of the lesion and was significantly higher (1,800–4,100 ppm) than in adjacent sound inner dentine (190–290 ppm). Fluoride concentrations varied markedly along the outer edge of both normal and carious root tissues. Fluoride increase at the lesion edge is not an effect of tissue shrinkage but probably a result of remineralisation events during caries. This additional F may be expected to increase tissue resistance to further acid attacks.

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Section: Discussionsupporting

confidence: 53%

Fluoride Distribution in Sound and Carious Root Tissues of Human Teeth

Shu

Pearce²,

Sissons³

et al. 1998

Caries Res

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“…Once the tooth formation is completed, dentinogenesis continues at a slower rate, resulting in secondary dentin production under physiological conditions, whereas tertiary dentin is formed in response to noxious external stimuli, being classified into reparative dentin and reactionary dentin (reviewed in Smith and Lesot 2001;Goldberg and Smith 2004;Moses et al 2006). During the physiologic response there is a continuous deposition of peritubular dentin, probably due also to defence mechanisms occurring during pathological insults such as caries (Vasiliadis et al 1983;Schüpbach et al 1992;Nanci, 2007). In particular, DMP1 has been shown to have similar functions as dentin sialophosphoprotein and to be detected in primary dentin with a marked decrease in tertiary dentin (Moses et al 2006).…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical localization of dentin matrix protein 1 in human dentin

Orsini¹,

Ruggeri²,

Mazzoni³

et al. 2009

Eur J Histochem

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Dentin matrix protein 1 (DMP1) is a non-collagenous matrix protein with a recognized role in the formation of mineralized tissues such as dentin. The aim of this study was to analyze the distribution of DMP1 in human dentin by means of immunofluorescence and high-resolution immunogold labeling. Fully developed, sound human dentin specimens were submitted to fluorescence labeling and post-embedding immunolabeling techniques with a rabbit polyclonal antihuman DMP1 antibody followed by corresponding fluorochrome-conjugated or gold-conjugated secondary antibodies. Both immunofluorescence and immunogold labeling showed an intense labeling associated with the peritubular dentin. In addition, at the ultrastructural level, there was also a moderate and diffuse immunoreaction over intertubular dentin, and a weak labeling within predentin which increased in density towards the mineralization front. This study suggests that in adult human teeth, like in rodents, DMP1 is prevalently concentrated at the level of peritubular dentin and this feature is preserved also in fully developed-teeth. These data are consistent with what has been observed in rodents and suggest that DMP1 plays a role in maintenance of the dentin tubular space.

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“…One of the specific dentine lesions that are observed in arrested dentine caries is a subsurface type of lesion with a well-mineralized surface layer [Knut and Helmut, 1962;Jones and Boyde, 1987;Schüpbach et al, 1989Schüpbach et al, , 1992. The mineral distribution in active advanced dentine lesions (natural dentine caries), so-called 'softened' type, is quite different from the subsurface type of lesion [Schüpbach et al, 1990].…”

Section: Methodsmentioning

confidence: 99%

Relationship between Mineral Distributions in Dentine Lesions and Subsequent Remineralization in vitro

Kawasaki

Ruben²,

Tsuda³

et al. 2000

Caries Res

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Though the mineral distribution of the dentine carious lesion varies largely from tooth to tooth and from patient to patient, there are two main distribution profiles that characterize natural carious lesions in dentine. These profiles include softened and subsurface lesion types. The mineral distribution relationship between the starting profile and the profile after remineralization is not known. In order to study the relational aspects, we have produced demineralized dentine samples in vitro with mineral profiles similar to those of typical natural carious lesions, and subsequently remineralized the samples in a remineralizing solution with various fluoride concentrations (0, 2 and 10 ppm F). The mineral distributions were obtained by using an improved microradiographic technique. In addition, the nature of deposited mineral was analyzed by diamond–coupled total internal reflectance spectroscopy. Definite relationship was observed between the original lesion mineral distribution and the mineral distributions following remineralization. The amount of mineral present in approximately the first 50 μm of the lesion influenced the overall mineral profile after remineralization, possibly through influencing ion transport. If the amount was high (> approximately 10 vol%), the deposited mineral was confined to the surface (0–50 μm). The original mineral at those depths acted like a nucleus of mineral regrowth when the amount of residual mineral was intermediate, and like a transport barrier when the surface layer was well mineralized. If a surface barrier was not present, mineral was deposited at deeper depths in the lesion. Fluoride effect on dentine remineralization was dependent on the original mineral content and its distribution in the lesion. Although a high concentration of fluoride was very effective in low–mineral lesions, it produced hyperremineralization on well–mineralized subsurface lesions so that it prevented effective remineralization especially in deeper lesions.

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Human Root Caries: Histopathology of Arrested Lesions

Cited by 68 publications

References 22 publications

Fluoride Distribution in Sound and Carious Root Tissues of Human Teeth

Fluoride Distribution in Sound and Carious Root Tissues of Human Teeth

Immunohistochemical localization of dentin matrix protein 1 in human dentin

Relationship between Mineral Distributions in Dentine Lesions and Subsequent Remineralization in vitro

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