Human S-antigen: peptide determinant recognition in uveitis patients

Tripathi, Parul; Saxena, Sandeep; Yadav, V. S.; Naik, Sita; Singh, Vijay K.

doi:10.1016/j.yexmp.2003.10.007

Cited by 16 publications

(19 citation statements)

References 32 publications

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“…It has been shown that a greatly heterogeneous T-cell proliferative response spectrum against S-Ag peptides in uveitis may be attributed to variation in disease entities and different stages of disease activity of the examined patients [6][7][8]. To exclude the above factors in the antigenic peptide mapping, we chose BD patients with a similar intensity of uveitis at the time of blood sampling.…”

Section: Discussionmentioning

confidence: 99%

“…To identify the immunodominant epitope of human S-Ag in uveitis, we used 40 overlapping peptides that are identical to the peptides used in earlier studies in uveitis patients [6][7][8]. The immune responses in those studies were Fig.…”

Section: Discussionmentioning

confidence: 99%

“…The SFC frequency was analyzed using the linear mixed-effects model fit by the maximum likelihood method tested by lymphocyte proliferation assay. If a stimulation index (SI) ≥2 was considered a positive reaction, almost all of the S-Ag peptides except for P12 induced a positive response in different patients with various uveitis entities [6][7][8]. In BD patients, as shown in Table 2 of their paper, de Smet et al [6] found that the responses to P18 and P40 were significantly higher than those in healthy controls.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, that concentration was used in this study. Since earlier studies showed that 20 μg/ml of S-Ag peptides induced an optimal lymphocyte proliferative responses in patients with uveitis [7,8], we simultaneously adopted this concentration for ELIspot assay in five out of eight patients. The results revealed highly heterogeneous responses against individual peptides at either concentration in all the patients (Fig.…”

Section: Assessment Of Cellular Immune Response To S-ag Peptides By Imentioning

confidence: 99%

“…Usually a typical immune response against a self protein is focused on one or two epitopes within that protein [5]. Immunodominant epitopes of human S-Ag have been investigated in a variety of uveitis entities through lymphocyte proliferation assay [6][7][8]. However, no peptide was found to induce a predominant immune response in all or most responders.…”

Section: Introductionmentioning

confidence: 99%

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Retinal S-antigen Th1 cell epitope mapping in patients with Behcet’s disease

Zhao

Yang

He³

et al. 2008

Graefes Arch Clin Exp Ophthalmol

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Background Retinal S-antigen (S-Ag) is a most characterized autoantigen of autoimmune uveitis. The recognized immunodominant epitope of human S-Ag in patients with uveitis has not been identified. In this study, we selected certain patients with active uveitis to map the Th1 cell epitope spectrum of human S-Ag in Behcet's disease(BD). Methods Blood samples were taken from eight active BD patients who showed an immune response to 40 mixed overlapping peptides spanning the entire sequence of human S-Ag. Peripheral blood mononuclear cells were isolated and stimulated with single S-Ag peptide at 5 μg/ml or 20 μg/ml. Single-cell immune responses were measured by IFN-γ ELIspot assay. Results BD patients heterogeneously responded to the SAg peptides at two concentrations. In general, the responses to 5 μg/ml peptides were slightly stronger than those to 20 μg/ml peptides, while the maximum SFC frequency to single peptide at the two concentrations was similar. Several peptides including P31, P35 and P40 induced a prominent response, with the frequency of S-Ag specific cells being about 0.007%. Significant reactivity pattern shift was noted in patients with different disease courses. Conclusions Certain active BD patients have S-Ag specific Th1 cells with a low frequency. The S-Ag epitope specificity between patients is highly heterogeneous, and varies with the uveitis course.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Assessment Of Cellular Immune Response To S-ag Peptides By Imentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Retinal S-antigen Th1 cell epitope mapping in patients with Behcet’s disease

Zhao

Yang

He³

et al. 2008

Graefes Arch Clin Exp Ophthalmol

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Tumor necrosis factor-α-mediated severity of idiopathic retinal periphlebitis in young adults (Eales’ disease): implication for anti-TNF-α therapy

Saxena

Pant

Khanna

et al. 2010

j ocul biol dis inform

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Autoimmunity, Autoinflammation, and Infection in Uveitis

Forrester

Kuffová

Dick

2018

American Journal of Ophthalmology

125

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Infection may be implicated directly or indirectly in many forms of noninfectious or undifferentiated uveitis. In addition to the growing recognition that foreign antigen, including reactivatable infectious agents, might hide within ocular tissues, the possibility that a dysregulated microbiome might generate T cells that cause immune-mediated ocular inflammation has now been demonstrated experimentally. An uncontrolled, overexuberant host immune response may cause continuing irreversible tissue damage even after the infection has been cleared.

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Human S-antigen: peptide determinant recognition in uveitis patients

Cited by 16 publications

References 32 publications

Retinal S-antigen Th1 cell epitope mapping in patients with Behcet’s disease

Retinal S-antigen Th1 cell epitope mapping in patients with Behcet’s disease

Tumor necrosis factor-α-mediated severity of idiopathic retinal periphlebitis in young adults (Eales’ disease): implication for anti-TNF-α therapy

Autoimmunity, Autoinflammation, and Infection in Uveitis

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