Human saphenous vein endothelial cell adhesion and expansion on micropatterned polytetrafluoroethylene

Abstract

Intimal hyperplasia and thrombosis are responsible for the poor patency rates of small-diameter vascular grafts. These complications could be avoided by a rapid and strong adhesion of endothelial cells to the prosthetic surfaces, which typically consist of expanded polytetrafluoroethylene (PTFE) for small-diameter vessels. We have previously described two peptide micropatterning strategies that increase the endothelialization rates of PTFE. The micropatterns were generated either by inkjet printing 300 μm squares or by spraying 10.1 ± 0.1 μm diameter droplets of the CGRGDS cell adhesion peptide, while the remaining surface was functionalized using the CWQPPRARI cell migration peptide. We now directly compare these two micropatterning strategies and examine the effect of hydrodynamic stress on human saphenous vein endothelial cells grown on the patterned surfaces. No significant differences in cell adhesion were observed between the two micropatterning methods. When compared to unpatterned surfaces treated with a uniform mixture of the two peptides, the cell expansion was significantly higher on sprayed or printed surfaces after 9 days of static cell culture. In addition, after 6 h of exposure to hydrodynamic stress, the cell retention and cell cytoskeleton reorganization on the patterned surfaces was improved when compared to untreated or random treated surfaces. These results indicate that micropatterned surfaces lead to improved rates of PTFE endothelialization with higher resistance to hydrodynamic stress.

Design, Degradation Mechanism and Long‐Term Cytotoxicity of Poly(l‐lactide) and Poly(Lactide‐co‐ϵ‐Caprolactone) Terpolymer Film and Air‐Spun Nanofiber Scaffold

“…HSVECs were isolated from healthy human saphenous vein segments removed during varicose vein stripping surgeries as previously described . All procedures were approved by the CHU de Québec ethics committee and with the informed consent of donors at the Saint‐François d'Assise Hospital in Québec city.…”

RGD and BMP-2 mimetic peptide crosstalk enhances osteogenic commitment of human bone marrow stem cells

“…The measured peptide densities were in agreement with those reported in some previous works. For example, Chollet et al [59] estimated the RGD peptide density at 1.7 pmol/mm² on polyethylene terephthalate (PET) surfaces, while Boivin et al [60] showed that the density of RGD peptides on polytetrafluoroehylene (PTFE) was 1.5 pmol/mm². Kim et al evaluated hMSCs osteogenic differentiation on modified tissue culture plastic (TCP) surfaces where the density of BMP-2 peptides was 0.69 pmol/mm² [61].…”

A fluorophore-tagged RGD peptide to control endothelial cell adhesion to micropatterned surfaces

“…Micropatterned glass surfaces were generated using the reaction scheme previously described for Teflon surfaces [22,25], except for the omission of the plasma treatment step and modification of the spray parameters. Commercially available aminated glass slides (treated with aminoalkysilane, Electron Microscopy Sciences, Hatfield, PA) circumvented the need for amination by plasma treatment.…”