Human serum albumin from recombinant DNA technology: Challenges and strategies

Chen, Zhe; He, Yan; Shi, Bo; Yang, Daichang

doi:10.1016/j.bbagen.2013.04.037

Cited by 80 publications

(58 citation statements)

References 117 publications

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“…[5][6][7] For the first time Cohn et.al purified albumin based on pH and ethanol precipitations from serum. Many researchers have tried to improve this method by several modifications.…”

Section: Discussionmentioning

confidence: 99%

Purification and Characterization of Bovine Serum Albumin Using Chromatographic Method

Balkani¹,

Shamekhi²,

Raoufinia³

et al. 2016

Adv Pharm Bull

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“…[5][6][7] For the first time Cohn et.al purified albumin based on pH and ethanol precipitations from serum. Many researchers have tried to improve this method by several modifications.…”

Section: Discussionmentioning

confidence: 99%

Purification and Characterization of Bovine Serum Albumin Using Chromatographic Method

Balkani¹,

Shamekhi²,

Raoufinia³

et al. 2016

Adv Pharm Bull

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“…And combination of homologous recombination with the phiC31 integrase strategy could increase the efficiency of integration at a desired locus (Ou et al 2013). The recombinant HSA can be highly expressed in various hosts and nearly identical to plasma HSA (Chen et al 2013;He et al 2011). Expression of recombinant HSA in the milk of transgenic cattle would provide an economical resource.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, annual demand for HSA for therapeutic application is more than 500 t worldwide. The high-dosage clinical applications and large market demand for HSA require high-level stable expression in milk of transgenic animals (Chen et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Production of transgenic cattle highly expressing human serum albumin in milk by phiC31 integrase-mediated gene delivery

et al. 2015

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Transgenic cattle expressing high levels of recombinant human serum albumin (HSA) in their milk may as an alternative source for commercial production. Our objective was to produce transgenic cattle highly expressing HSA in milk by using phiC31 integrase system and somatic cell nuclear transfer (SCNT). The mammary-specific expression plasmid pIACH(-), containing the attB recognition site for phiC31 integrase, were co-transfected with integrase expression plasmid pCMVInt into bovine fetal fibroblast cells (BFFs). PhiC31 integrase-mediated integrations in genome of BFFs were screened by nested inverse PCR. After analysis of sequence of the PCR products, 46.0% (23/50) of the both attB-genome junction sites (attL and attR) were confirmed, and four pseudo attP sites were identified. The integration rates in BF3, BF11, BF19 and BF4 sites were 4.0% (2/50), 6.0% (3/50), 16.0% (8/50) and 20.0% (10/50), respectively. BF3 is located in the bovine chromosome 3 collagen alpha-3 (VI) chain isomer 2 gene, while the other three sites are located in the non-coding region. The transgenic cell lines from BF11, BF19 and BF4 sites were used as donors for SCNT. Two calves from transgenic cells BF19 were born, one died within a few hours after birth, and another calf survived healthy. PCR and Southern blot analysis revealed integration of the transgene in the genome of cloned calves. The nested reverse PCR confirmed that the integration site in cloned calves was identical to the donor cells. The western blotting assessment indicated that recombinant HSA was expressed in the milk of transgenic cattle and the expression level was about 4-8 mg/mL. The present study demonstrated that phiC31 integrase system was an efficient and safety gene delivery tool for producing HSA transgenic cattle. The production of recombinant HSA in the milk of cattle may provide a large-scale and cost-effective resource.

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“…In the last few decades, DNA recombinant technology has shown great potential in biomedical, agriculture, food production, and system biology [3][4][5][6]. DNA recombinant may be regarded as another wave of post-modern industrial revolution.…”

Section: Introductionmentioning

confidence: 99%

Malaysia Biosafety Act 2007 and Cartagena Protocol on Biosafety: A critical comparative analysis

Chan

2016

Preprint

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Protocol. It is concluded that Malaysia biosafety regulation system satisfies this international requirement. However, with regards to enforcement of this Act, it remains unanswered, as no precedent has been reported. Interestingly, non-compliance of some proviso in the Biosafety Act 2007 will result in criminal penalty, and its impacts on the research and development in the biotechnology industry, commercial investment from abroad and domestic markets, and international trading of LMOs as food and feed, remain to be seen.

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Human serum albumin from recombinant DNA technology: Challenges and strategies

Cited by 80 publications

References 117 publications

Purification and Characterization of Bovine Serum Albumin Using Chromatographic Method

Purification and Characterization of Bovine Serum Albumin Using Chromatographic Method

Production of transgenic cattle highly expressing human serum albumin in milk by phiC31 integrase-mediated gene delivery

Malaysia Biosafety Act 2007 and Cartagena Protocol on Biosafety: A critical comparative analysis

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