Purification and Characterization of Bovine Serum Albumin Using Chromatographic Method

Balkani, Sanaz; Shamekhi, Sara; Raoufinia, Ramin; Abdolalizadeh, Jalal

doi:10.15171/apb.2016.080

Cited by 16 publications

(8 citation statements)

References 21 publications

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“…92,93 Clinical administration of mAbs well-tolerated normally through intravenous and subcutaneous. 8 The mAbs have several applications including Cancer Immunotherapy, 91,94 Bone Marrow and Organ Transplantation, 95,96 Protein Purification, [97][98][99] Disease Diagnosis in immunoassay tests including enzymelinked immune sorbent assay (ELISA), western blotting, immunohistochemistry and immunofluorescence tests. 100,101 Our team has been developed the mAbs against a wide range of antigens including TNF-α, 102 CD20, 103 CD34, 104,105 receptor tyrosine kinase-like orphan receptor proteins, 106 human epidermal growth factor receptor, 107 heat shock protein 70, 108 vascular endothelial growth factor receptor 2, 77,78 human IgG and IgA.…”

Section: Monoclonal Antibodiesmentioning

confidence: 99%

PCSK9: A Key Target for the Treatment of Cardiovascular Disease (CVD)

et al. 2020

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Proprotein convertase subtilisin/kexin type 9 (PCSK9), as a vital modulator of low-densitylipoprotein cholesterol (LDL-C) , is raised in hepatocytes and released into plasma where it bindsto LDL receptors (LDLR), leading to their cleavage. PCSK9 adheres to the epidermal growthfactor-like repeat A (EGF-A) domain of the LDLR which is confirmed by crystallography. LDLRexpression is adjusted at the transcriptional level through sterol regulatory element bindingprotein 2 (SREBP-2) and at the post translational stages, specifically through PCSK9, and theinducible degrader of the LDLR PCSK9 inhibition is an appealing new method for reducing theconcentration of LDL-C. In this review the role of PCSK9 in lipid homeostasis was elucidated, theeffect of PCSK9 on atherosclerosis was highlighted, and contemporary therapeutic techniquesthat focused on PCSK9 were summarized. Several restoration methods to inhibit PCSK9 havebeen proposed which concentrate on both extracellular and intracellular PCSK9, and theyinclude blockage of PCSK9 production by using gene silencing agents and blockage of it’sbinding to LDLR through antibodies and inhibition of PCSK9 autocatalytic processes by tinymolecule inhibitors.

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Section: Monoclonal Antibodiesmentioning

confidence: 99%

PCSK9: A Key Target for the Treatment of Cardiovascular Disease (CVD)

et al. 2020

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“…2B (ii), lane 8). BSA standards were also used in other studies to achieve high quality products of unique proteins purified for assessing immunoaffinity and antibody production (Balkani et al, 2016;Raoufinia et al, 2016).…”

Section: Sds-page Analysis and Purification Of Proteins From Differenmentioning

confidence: 99%

ELISA-Based Development of Functional Antibodies for the Efficient Detection of Mycotoxigenic Fusarium (F. oxysporum and F. circinatum) and Diplodia (D. pinea) Species

Mkhize¹,

Mangena²

2020

American Journal of Biochemistry and Biotechnology

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Fusarium contains the most toxigenic and important fungal pathogens, producing a wide range of mycotoxins that include the more toxic secondary metabolites called fumonisins. This study analyzed the presence of common exo-and mycelium-soluble antigens, purified proteins using reducing SDS-PAGE and the expression of antibodies against different fungal pathogens. The results showed the presence of common antigens between pathogens and development of antibodies against antigens from F. circinatum, F. oxysporum and D. pinea. A strong reactivity against the antigens ranged between 0.510 to 0.868 in F. circinatum and F. oxysporum isolates. The reactivity of anti-D. pinea antibodies was relatively low, with the highest absorbance value of 0.650, accompanied by a minimal cross reactivity. Overall, these findings indicated that, antibodies against mycelium-soluble antigens were the most immunoreactive and could be helpful for the development of a rapid Fusarium detection tool for use in various industries.

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“… 1 Carrier proteins act by multimerizing subunit vaccines, which promotes B cell receptor cross-linking and uptake by dendritic cells. 2 , 3 There are clinically tested carrier proteins, 4 − 6 like keyhole limpet hemocyanin (KLH) isolated directly from keyhole limpets, 1 , 7 and bovine serum albumin 1 purified by fractionation of bovine plasma, 8 and clinically licensed carriers like diphtheria toxoid 4 , 9 , 10 and tetanus toxoid. 4 , 11 Although anti-carrier immunity 12 can be a concern, carrier proteins have proven effective in amplifying the immunity generated by subunit vaccines.…”

Section: Introductionmentioning

confidence: 99%

Chemically Modified Bacterial Sacculi as a Vaccine Microparticle Scaffold

et al. 2022

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Vaccine scaffolds and carrier proteins increase the immunogenicity of subunit vaccines. Here, we developed, characterized, and demonstrated the efficacy of a novel microparticle vaccine scaffold comprised of bacterial peptidoglycan (PGN), isolated as an entire sacculi. The PGN microparticles contain bio-orthogonal chemical handles allowing for site-specific attachment of immunogens. We first evaluated the purification, integrity, and immunogenicity of PGN microparticles derived from a variety of bacterial species. We then optimized PGN microparticle modification conditions; Staphylococcus aureus PGN microparticles containing azido- d -alanine yielded robust conjugation to immunogens. We then demonstrated that this vaccine scaffold elicits comparable immunostimulation to the conventional carrier protein, keyhole limpet hemocyanin (KLH). We further modified the S. aureus PGN microparticle to contain the SARS-CoV-2 receptor-binding domain (RBD)—this conjugate vaccine elicited neutralizing antibody titers comparable to those elicited by the KLH-conjugated RBD. Collectively, these findings suggest that chemically modified bacterial PGN microparticles are a conjugatable and biodegradable microparticle scaffold capable of eliciting a robust immune response toward an antigen of interest.

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Purification and Characterization of Bovine Serum Albumin Using Chromatographic Method

Cited by 16 publications

References 21 publications

PCSK9: A Key Target for the Treatment of Cardiovascular Disease (CVD)

PCSK9: A Key Target for the Treatment of Cardiovascular Disease (CVD)

ELISA-Based Development of Functional Antibodies for the Efficient Detection of Mycotoxigenic Fusarium (F. oxysporum and F. circinatum) and Diplodia (D. pinea) Species

Chemically Modified Bacterial Sacculi as a Vaccine Microparticle Scaffold

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