Human Serum Mannose Binding Protein (MBP): Development of an Enzyme-Linked Immunosorbent Assay (ELISA) and Determination of Levels in Serum from 1085 Normal Japanese and in Some Body Fluids

Terai, Itaru; Kobayashi, Keiko; Fujita, Takashi; Hagiwara, Kaoru

doi:10.1006/bmmb.1993.1052

Cited by 64 publications

(65 citation statements)

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“…Elevated levels of MBL have been reported in a single study of patients with chronic renal failure of unspecified causes (29). In that study there was no relationship between serum MBL levels and glomerular filtration rate, and because MBL is not excreted or degraded by the kidneys (30), it is unlikely that differences in MBL levels between the nephropathy and normoalbuminuric groups are caused by differences in renal function. It has been suggested that patients with type 1 diabetes are characterized by a state of chronic low-grade inflammation (31).…”

Section: Discussionmentioning

confidence: 86%

Association Between Mannose-Binding Lectin and Vascular Complications in Type 1 Diabetes

et al. 2004

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Complement activation and inflammation have been suggested in the pathogenesis of diabetic vascular lesions. We investigated serum mannose-binding lectin (MBL) levels and polymorphisms in the MBL gene in type 1 diabetic patients with and without diabetic nephropathy and associated macrovascular complications. Polymorphisms in the MBL gene and serum MBL levels were determined in 199 type 1 diabetic patients with overt nephropathy and 192 type 1 diabetic patients with persistent normoalbuminuria matched for age, sex, and duration of diabetes, as well as in 100 healthy control subjects. The frequencies of high-and low-expression MBL genotypes were similar in patients with type 1 diabetic and healthy control subjects. High MBL genotypes were significantly more frequent in diabetic patients with nephropathy than in the normoalbuminuric group, and the risk of having nephropathy given a high MBL genotype assessed by odds ratio (OR) was 1.52 (1.02-2.27, P ‫؍‬ 0.04). Median serum MBL concentrations were significantly higher in patients with nephropathy than in patients with normoalbuminuria: 2,306 g/l (interquartile range [IQR] 753-4,867 g/l) vs. 1,491 g/l (577-2,944 g/l), P ‫؍‬ 0.0003. In addition, even when comparing patients with identical genotypes, serum MBL levels were higher in the nephropathy group than in the normoalbuminuric group. Patients with a history of cardiovascular disease had significantly elevated MBL levels independent of nephropathy status (3,178 g/l [IQR 636 -5,231 g/l] vs. 1,741 g/l [656 -3,149 g/l], P ‫؍‬ 0.02). The differences in MBL levels between patients with and without vascular complications were driven primarily by pronounced differences among carriers of high MBL genotypes (P < 0.0001). Our findings suggest that MBL may be involved in the pathogenesis of micro-and macrovascular complications in type 1 diabetes, and that determination of MBL status might be used to identify patients at increased risk of developing these complications. Diabetes 53

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Section: Discussionmentioning

confidence: 86%

Association Between Mannose-Binding Lectin and Vascular Complications in Type 1 Diabetes

et al. 2004

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“…We have previously reported that MBL was detected in all Japanese sera examined and no MBL deficiency was found with our enzyme-linked immunosorbent assay (ELISA) system [26,27]. However, there have been a number of reports concerning MBL deficiencies in individuals of other countries [8,9,19,20,22,28].…”

Section: Introductionmentioning

confidence: 86%

“…MBL levels were determined by a sandwich ELISA as described previously [26,27]. In brief, microtiter plates (Maxisorp TM ; Nunc A/S, Roskilde, Denmark) were coated with a polyclonal anti-MBL rabbit IgG in PBSN (0.02 M PBS containing 0.05% NaN 3 ).…”

Section: Mbl Concentrationmentioning

confidence: 99%

Relationship between gene polymorphisms of mannose‐binding lectin (MBL) and two molecular forms of MBL

et al. 2003

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Mannose-binding lectin (MBL) activates complement through MBL-associated serine proteases (MASP). A deficiency in MBL due to mutations at exon 1 of the human MBL gene is reported to cause vulnerability to infection. We examined sera of known MBL genotype by gel filtration and assessed their elution patterns using an ELISA for MBL and identified two MBL forms, a high-molecular-mass form and a lower-molecular-mass form. By the identification of either or both forms in individual sera, three types of patterns emerged: type 1 consisted of a high-molecular form; type 2, of a low-molecular form; and type 3, of both forms. Types 1, 2 and 3 corresponded, respectively, to a wild type (A/A), a homozygous mutation at codon 54 (B/B) and their heterozygote (A/B). One exception was a heterozygous LXPA/LYPB phenotype that exhibited the type-2 pattern. Binding to mannan and MASP-1/3 occurred exclusively with the high-molecular form. An apparent MBL deficiency does not in fact represent a deficiency in MBL molecules but rather the presence of circulating oligomeric mutant MBL with impaired function.

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“…Blood samples of the cases in HD program were taken on the day of HD session, just before the HD treatment. However, we assume that the day on which blood sample is taken will not affect the results, since MBL levels, having a quite stable course, show no circadian change and are not affected by dialysis practice and residual renal functions (13,19).…”

Section: Discussionmentioning

confidence: 99%

The Effect of Serum Mannose-Binding Lectin Levels on Dialysis- Related Peritonitis and Catheter-Related Bacteremia

Erken¹,

Torun²,

Sezgin³

et al. 2015

Turk Neph Dial Transpl

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OBJECTIVE: Mannose-binding lectin (MBL) takes part in innate immunity through opsonisation and complement activation. Deficiency of MBL is associated with some infections and autoimmune disorders. This study focused on functional MBL deficiency and its effects on dialysis-related peritonitis and catheter-related bacteremia in patients with end stage renal disease. MATERIAL and METHODS:The study included 51 patients on chronic peritoneal dialysis (PD) program and 31 under maintenance hemodialysis (HD) who had tunneled/cuffed hemodialysis catheters (total 82). Serum MBL level measurements were performed by ELISA technique. RESULTS:The mean value for serum MBL in patient groups of PD, HD, and healthy controls were 2536.5 ng/ml, 2088.7 ng/ml, 1924 ng/ml respectively. Difference of MBL level was not significant among groups. Serum MBL value was negatively correlated to the number of peritonitis episodes in PD group (p=0.019). Deficiency of MBL was not associated with high incidence of peritonitis. Surveillance of catheter associated blood stream infection for tunneled/cuffed hemodialysis catheters was 2.07 episodes/1000 catheter days. An association with MBL deficiency and incidence of catheter-related bacteremia was not observed. CONCLUSION:Serum MBL value was negatively correlated to the number of peritonitis episodes but an expected association of MBL deficiency with high incidence of dialysis-related peritonitis and catheter-related bacteremia was not found. New studies with greater sample size might probably indicate the potential effect of MBL deficiency on dialysis-related peritonitis. KEY WORDS:Mannose-binding lectin, Peritoneal dialysis, Peritonitis, Hemodialysis, Catheterrelated bacteremia ÖZ AMAÇ: Mannoz bağlayıcı lektin (MBL), opsonizasyon ve kompleman aktivasyonu aracılığı ile doğal immüniteye katkıda bulunmaktadır. MBL eksikliği bazı enfeksiyöz ve otoimmün hastalıklarla ilişkilidir. Çalışmamızda, kronik periton diyalizi (PD) hastaları ve vasküler giriş yolu olarak tünelli kateter kullanılan hemodiyaliz hastalarında fonksiyonel MBL eksikliğinin enfeksiyon gelişimi ile ilişkisini incelemeyi amaçladık. GEREÇ ve YÖNTEMLER:Çalışmaya kronik PD programındaki 51 hasta ve tünelli/kaflı kateter kullanılarak kronik hemodiyaliz (HD) programına devam eden 31 hasta olmak üzere toplam 82 hasta dahil edildi. Bu gruplara 35 sağlıklı kontrol ilave edildi. Hasta ve kontrollerin serum örneklerinde ELISA yöntemi uygulanarak MBL seviyesi ölçüldü. Serum MBL düzeyi ve fonksiyonel MBL eksikliği ile peritonit ve tünelli kateter enfeksiyonu arasındaki ilişki araştırıldı.BULGULAR: Serum MBL düzeyi ortalama değeri PD grubunda 2536,5 ng/ml, HD grubunda 2088,7 ng/ml ve sağlıklı kontrol grubunda 1924 ng/ml olarak bulundu. Gruplar arasında; serum MBL düzeyi yönünden farklılık yoktu. PD grubunda serum MBL düzeyi ile peritonit sayısı arasında negatif korelasyon mevcuttu (p=0,019). PD grubunda yıllık peritonit insidansı yüksek olan (>0,67 episod/ yıl) olguların ortalama MBL değeri, diğer olgulardan daha düşük olsa da, fonksiyonel...

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Human Serum Mannose Binding Protein (MBP): Development of an Enzyme-Linked Immunosorbent Assay (ELISA) and Determination of Levels in Serum from 1085 Normal Japanese and in Some Body Fluids

Cited by 64 publications

References 0 publications

Association Between Mannose-Binding Lectin and Vascular Complications in Type 1 Diabetes

Association Between Mannose-Binding Lectin and Vascular Complications in Type 1 Diabetes

Relationship between gene polymorphisms of mannose‐binding lectin (MBL) and two molecular forms of MBL

The Effect of Serum Mannose-Binding Lectin Levels on Dialysis- Related Peritonitis and Catheter-Related Bacteremia

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Human Serum Mannose Binding Protein (MBP): Development of an Enzyme-Linked Immunosorbent Assay (ELISA) and Determination of Levels in Serum from 1085 Normal Japanese and in Some Body Fluids

Cited by 64 publications

References 0 publications

Association Between Mannose-Binding Lectin and Vascular Complications in Type 1 Diabetes

Association Between Mannose-Binding Lectin and Vascular Complications in Type 1 Diabetes

Relationship between gene polymorphisms of mannose‐binding lectin (MBL) and two molecular forms of MBL

The Effect of Serum Mannose-Binding Lectin Levels on Dialysis- ￼Related Peritonitis and Catheter-Related Bacteremia

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The Effect of Serum Mannose-Binding Lectin Levels on Dialysis- Related Peritonitis and Catheter-Related Bacteremia