Human Serum Protein Adsorption onto Synthesis Nano-Hydroxyapatite

Abstract

Adsorption of human serum proteins (Albumin and total protein) onto high purity synthesis nano-hydroxyapatite (HA), Ca₁₀(PO₄)₆(OH)₂, has been studied in a wide temperature range by UV-visible spectrophotometer. Adsorption isotherm is basically important to describe how solutes interact with adsorbent, and is critical in optimizing the use of adsorbent. In the present study, the experimental results were fitted to the Langmuir, Freundlich, Temkin and Dubinin-Radushkevich (DR) models to obtain the characteristic parameters of each model and square of the correlation coefficients (R²). According to the results, the DR isotherm model had the best agreement with the experimental data. The effect of temperature on adsorption of human serum proteins (HSP) onto the synthesized nano-HA was studied. The experimental results indicated that temperature increase generally causes an increase in the adsorption of HSP onto the nano-HA. This is basically due to the effect of temperature on the HSP activity and its diffusion rate on HA surfaces.

Environmental pH-controlled loading and release of protein on mesoporous hydroxyapatite nanoparticles for bone tissue engineering

“…The results demonstrated that MHA had a greatly improving protein loading capacity, nearly double that of HA. A number of factors are important in determining the amount of protein adsorbed on surfaces [6,10,16,17,20]. A higher surface area generally corresponds to a higher amount of protein adsorption [10,16].…”

“…Hydroxyapatite particles [HA, Ca 10 (PO 4 ) 6 (OH) 2 )] with various morphologies and surface properties have been investigated as protein delivery device due to their biocompatible, nontoxic, and noninflammatory properties [11,[14][15][16][17]. Protein loading on HA is carried out by surface physical adsorption.…”

“…The adsorption behaviors of large biomolecules onto hydroxyapatite have been frequently studied [16,17,19,22]. To our knowledge, attention has been focused on the amount of adsorption for large biomolecules, while models of adsorption onto MHA have scarcely been mentioned in the previous papers.…”

Porous and spherical hydroxyapatite microcomposites for immunoglobulin G adsorption

“…Up to 45°C, the adsorption was efficient, and there was a slight increase in the adsorption capacity by about 20% because of the increasing diffusion rate into the microcomposites [poly(EGDMA) had a negligible adsorption capacity toward IgG]. An increase in the temperature increased the adsorption capacity of the porous and spherical HA microcomposites because of the endothermic and spontaneous behavior of the adsorption process, as discussed by Mohsen Nia et al…”

Enhanced dissolution and stability of Tanshinone IIA base by solid dispersion system with nano-hydroxyapatite

“…[1617] In addition, hydroxyapatite has been used as an adsorbent for a wide range of ions, small molecules, and macromolecules. [1819] Nano-hydroxyapatite (n-HAp) exhibits a significant adsorption capacity owing to its relatively large surface area[20] and was found to be a superior sorbent because of its low cost, availability, and high defluoridation capacity, as previously reported by Sairam Sundaram et al . [21] n-HAp has also received significant attention for application in medicine and dentistry.…”