Human tear proteomics and peptidomics in ophthalmology: Toward the translation of proteomic biomarkers into clinical practice

Azkargorta, Mikel; Soria, Javier Millán; Acera, Arantxa; Iloro, Ibón; Elortza, Félix

doi:10.1016/j.jprot.2016.05.006

Cited by 82 publications

(88 citation statements)

References 97 publications

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“…Since tear fluid contains thousands of proteins -the most abundant of which are lysozyme, lactoferrin, and albumin -noninvasive tear monitoring could also be used to detect protein biomarkers correlated with various diseases. Proteomic analysis of tear samples may be one approach to identify biomarkers linked to ocular diseases (21)(22)(23)(24)(25). However, the correlation between the concentration in tears and blood and its relevance to disease progression must be extensively validated before using novel tear analytes.…”

Section: Advantages Of Tears As a Biomarker Sourcementioning

confidence: 99%

Lab on the eye: A review of tear-based wearable devices for medical use and health management

Lan

Yang

2019

BST

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Wearable sensors have garnered considerable interest because of their great promise in terms of personalized health and disease management. Tears are a superior target for wireless, non-invasive wearable devices, and tear-based platforms have developed rapidly over the past decade. Although an increasing number of tear analytes have been found to be associated with multiple diseases, glucose still serves as a main target for tearbased wearable devices. There has been much investment and efforts to develop tearbased wearable biosensors, with contact lens-based and spring-like sensors flourishing commercially. Current efforts have moved past ocular and systematic disease markers to nutrients and chemicals. Moreover, tear-based wearable devices also have the potential to treat some ocular diseases. This review discusses aspects of tear-based wearable devices and it emphasizes that strict clinical validation is needed before such platforms enter the market. Multifunctional and theranostic strategies would further broaden their clinical use in the future.

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Section: Advantages Of Tears As a Biomarker Sourcementioning

confidence: 99%

Lab on the eye: A review of tear-based wearable devices for medical use and health management

Lan

Yang

2019

BST

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“…Even though substantial efforts have been made in the analysis of naturally occurring peptides in tears, their characterization and biological roles remain elusive with around 300 native peptides identified so far (Figure ), some of which seem to have antimicrobial activity. The tear film provides the first barrier against the bacterial, fungal, and viral pathogens . Curiously, only 26 precursor proteins were reported, such as cystatins, dermicidin, PRP4, and thymosin beta‐4.…”

Section: Comparative Analysis Of the Biological Processes Modulated Bmentioning

confidence: 99%

Digging Deep into Peptidomics Applied to Body Fluids

Vitorino

2018

Proteomics

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Peptidomics techniques have allowed the identification of thousands of peptides that are derived from proteins in body fluids, despite the considerable challenges behind sample handling, MS-based identification, data analysis, and integration with bioinformatics tools. Body fluids' naturally occurring peptides are known to perform a variety of local and systemic functions; however, its knowledge is limited. Even so, the biological meaning that can be retrieved from peptidomics applied to the identification of disease markers and to the development of therapies using peptides has driven the progresses made in this field. In this review, a comparative analysis of body fluids' peptidome data retrieved from databases and from scientific papers is performed to identify the biological processes modulated by naturally occurring peptides. This integrative analysis highlights several interesting facts, such as the small overlap between blood-derived serum and plasma, which illustrates the impact of sample handling on these fluids peptidome. Urine is the body fluid with more naturally occurring peptides identified so far, most of which are derived from collagens. In saliva, the majority of peptides are originated from extracellular matrix proteins. Cerebrospinal fluid presents a high number of peptides derived from distinct proteins, mostly involved in the regulation of nervous system homeostasis. The lowest number of endogenous peptides was found in tears, most of which present antimicrobial activity. Collectively, data analysis highlights a peptidome signature for each body fluid, which comprehension will certainly help to improve disease management.

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“…Tear fluid comprises electrolytes, lipids, mucins, and proteins among other components 1. Tear composition reflects the physiological condition of underlying tissue but can be altered in disease related to the eye or other organs and systemic diseases such as diabetes 2.…”

Section: Figurementioning

confidence: 99%

“…A major constituent of tear fluid is proteins that fulfill various functions 1. For the tear fluid proteome, up to around 1500 proteins have been identified in deep proteomic studies 7.…”

Section: Figurementioning

confidence: 99%

Comparison of tear proteome in allergic rhinoconjunctivitis patients and controls with respect to pollen season

Tomazic

Liesinger

Pucher

et al. 2018

Allergy

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Human tear proteomics and peptidomics in ophthalmology: Toward the translation of proteomic biomarkers into clinical practice

Cited by 82 publications

References 97 publications

Lab on the eye: A review of tear-based wearable devices for medical use and health management

Lab on the eye: A review of tear-based wearable devices for medical use and health management

Digging Deep into Peptidomics Applied to Body Fluids

Comparison of tear proteome in allergic rhinoconjunctivitis patients and controls with respect to pollen season

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