Human testicular mast cells contain tryptase: increased mast cell number and altered distribution in the testes of infertile men

Meineke, Viktor; Frungieri, Mónica Beatriz; Jessberger, B.; Vogt, Hermann-J.; Mayerhofer, Artur

doi:10.1016/s0015-0282(00)00626-9

Cited by 151 publications

(166 citation statements)

References 33 publications

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“…The present results are similar to those published by others (27)(28)(29) who highlighted the increased number of mast cells in testicular lesions associated with infertility. Meineke et al (29) observed, as we did, a shift of mast cells from interstitium to tubular wall. Hashimoto et al (27) used a mast cell blocker for the treatment of patients with idiopathic infertility.…”

Section: Discussionsupporting

confidence: 93%

Mast cells in testicular lesions

Kollur¹,

Pattankar²,

Hag³

2004

Upsala Journal of Medical Sciences

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Section: Discussionsupporting

confidence: 93%

Mast cells in testicular lesions

Kollur¹,

Pattankar²,

Hag³

2004

Upsala Journal of Medical Sciences

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“…The mast cells and plasma cells were not observed in both groups of testes in the present study. However, the presence of mast cells is considered as very important for normal spermatogenesis in man (Meineke et al, 2000). Few fibroblasts were found in both groups.…”

Section: Resultsmentioning

confidence: 89%

Morphology of the Interstitial Tissue of Active and Resting Testis of the Guinea Fowl

et al. 2017

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DHARANI, P.; KUMARY, S. U.; SUNDARAM, V.; JOSEPH, C. & RAMESH, G.Morphology of the interstitial tissue of active and resting testis of the Guinea fowl. Int. J. Morphol., 35(4):1359Morphol., 35(4): -1362Morphol., 35(4): , 2017. SUMMARY:The morphology of the interstitial tissue of sexually active and resting testis of the guinea fowl were studied. Six adult health birds of active and resting phases of reproductive cycle were used for this study. The interstitial tissue consisted of loose connective tissue, interstitial cells (Leydig cells), few connective cells, blood vessels and adrenergic nerve fibres in the present study in both active and resting testes. The interstitial tissue was compact in sexually active testis whereas, the volume of the tissue was found to be increased in resting testis. The loose connective tissue of the interstitial tissue composed of mainly of collagen fibres and few reticular fibres whereas elastic fibres were absent in both groups studied. The interstitial cells appeared in clusters of a few cells and were relatively less in the active testis than the resting testis. The interstitial cells were pale staining or polygonal cells with euchromatic nuclei with few large lipid droplets in the active testis whereas the cells were flat and highly heterochromatic with numerous small lipid droplets in resting testis. Few macrophages were found only in resting testis. Interstitial cells showed negative reaction to alkaline, acid phosphatases and PAS in both groups studied but positive for lipids. The interstitial tissue was well vascularised with centrally located blood vessels in the active testis whereas the blood vessels were small and inconspicuous in the resting testis. The lymphatic vessels were not identified in both groups studied.

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“…To examine whether this newly identified pathway may be of relevance to a human fibrotic disorder, we studied human testicular biopsies from men presenting normal, mildly, or severely impaired spermatogenesis (18). We studied this tissue, because it is well documented (18,(29)(30)(31)(32)(33)) that interstitial and peritubular tryptase-positive mast cells are 2-3 times increased and show signs of activation͞ degranulation in these cases (Fig.…”

Section: Identification Of the Components Required For The Tryptase͞cmentioning

confidence: 99%

“…Nevertheless this condition attracted our interest, because coinciding with fibrotic changes activated, tryptase-producing mast cells are increased in testes of subfertile and infertile men (18). We speculated that these events may be causally linked and that mast cells via tryptase may be able to initiate fibrosis.…”

mentioning

confidence: 99%

Proliferative action of mast-cell tryptase is mediated by PAR2, COX2, prostaglandins, and PPARγ: Possible relevance to human fibrotic disorders

Frungieri

Weidinger

Meineke

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

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238

229

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Mast-cell products can stimulate fibroblast proliferation, implying that these cells are key players in fibrosis. One mast-cell product, the serine protease tryptase, is known to activate protease-activated receptor 2 (PAR2) and cause proliferation of fibroblasts. We found that recombinant tryptase, human mastcell (HMC-1) supernatant, which contains tryptase, and the PAR2-activating peptide SLIGKV exert fibroproliferative actions in human fibroblasts. Here we report insights into this action, which after activation of PAR2 leads to increased expression of cyclooxygenase 2 (COX2), a key enzyme in the biosynthesis of prostaglandins, and consequently to enhanced prostaglandin synthesis. Subsequent cell proliferation is mediated by the prostaglandin 15-deoxy-⌬ 12,14 -prostaglandin J2, which acts via the nuclear peroxisome proliferator-activated receptor ␥ (PPAR␥). Fibroblast proliferation induced by tryptase and PAR2 agonist peptide can be blocked by antagonists of COX2 and PPAR␥, implying that the proliferative effect of tryptase is PAR2-initiated but depends on COX2, 15-deoxy-⌬ 12,14 -prostaglandin J2, and PPAR␥. This previously uncharacterized pathway could be of relevance for human fibrotic diseases. For instance, increased numbers of activated mast cells are correlated with fibrosis in testes of infertile men. In these cases all components of the signaling pathway of tryptase were detected as well as expression of COX2. Therefore, our study describes as-yet-unknown interactions between mast cells and fibroblasts, which could be relevant for human fibrotic diseases.

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Human testicular mast cells contain tryptase: increased mast cell number and altered distribution in the testes of infertile men

Cited by 151 publications

References 33 publications

Mast cells in testicular lesions

Mast cells in testicular lesions

Morphology of the Interstitial Tissue of Active and Resting Testis of the Guinea Fowl

Proliferative action of mast-cell tryptase is mediated by PAR2, COX2, prostaglandins, and PPARγ: Possible relevance to human fibrotic disorders

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