Human umbilical cord derived stem cells for the injured heart

Wu, Kai; Yang, Shangda; Zhou, Bin; Du, Wei; Gu, Dong Sheng; Liu, Peng Xia; Liao, Wen Bin; Han, Zhongchao; Liu, Ying Long

doi:10.1016/j.mehy.2006.06.038

Cited by 16 publications

(7 citation statements)

References 9 publications

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“…So, in this study, the ability of several cocktails to differentiate hUCM cells into neural cells was compared. We found the cocktail consisting of DMEM/ LG, FBS, RA, FGF, and EGF (DF/R/Fg/E) induced hUCM cells to express the highest percentage of In this study, we selected the UC-derived MSCs as a new source of stem cells (Wu et al 2007), Studies have shown that MSCs can easily be cultivated for more than 3 months without any senescence evidence and reduction of differentiation potential (Leeb et al 2009). Of course, some factors such as; basal nutrients, cell density, growth factors and cytokines, all play significant roles in MSC differentiation (Bobis et al 2006).…”

Section: Discussionmentioning

confidence: 99%

“…MSCs isolated from Wharton's Jelly (hUCMs (were shown to have surface markers, immune properties (Fong et al 2011;Weiss et al 2006), and differentiation potential towards cells with mesoderm offspring (Penolazzi et al 2009;Schneider et al 2010), including adipose, bone, cartilage, skeletal muscle, endothelium, cardiomyocyte and neuronal cells (Ishige et al 2009;Leeb et al 2009;Penolazzi et al 2009;Wu et al 2007), allowing replacement of ectodermal and mesodermal tissues (Schneider et al 2010). Several reports have characterized the in vitro differentiation capabilities of hUCM cells (La Rocca et al 2009) into neuron like cells (Abdulrazzak et al 2010;Cao and Feng 2009;Hu et al 2009;Zhang et al 2009b).…”

Section: Introductionmentioning

confidence: 99%

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Neural differentiation of human umbilical cord matrix-derived mesenchymal cells under special culture conditions

et al. 2014

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Many neural disorders are characterized by the loss of one or several types of neural cells. Human umbilical cord-derived mesenchymal cells (hUCMs) are capable of differentiating into neuron, astroglia-like and oligodendrocyte cell types. However, a reliable means of inducing the selective differentiation of hUCMs into neural cells in vitro has not yet been established. For induction of neural differentiation, hUCMs were seeded onto sterile glass slides and six various cocktails using a base medium (DMEM/LG) supplemented with 10 % FBS, retinoic acid (RA), dimethyl sulfoxide (DMSO), epidermal growth factor (EGF) and fibroblast growth factor (FGF) were used to compare their effect on neuronal, astrocyte and oligodandrocyte differentiation. The hUCMs were positive for mesenchymal markers, while they were negative for hematopoietic markers. Differentiation to adipogenic and osteogenic lineage was detected in these cells. Our data revealed that the cocktail consisting of DMEM/LG, FBS, RA, FGF, and EGF (DF/R/Fg/E group) induced hUCM cells to express the highest percentage of nestin, ß-tubulin III, neurofilament, and CNPase. The DF/Ds/Fg/E group led to the highest percentage of GFAP expression. While the expression levels of NF, GFAP, and CNPase were the lowest in the DF group. The least percentage of nestin and ß-tubulin III expression was observed in the DF/Ds group. We may conclude that FGF and EGF are important inducers for differentiation of hUCMs into neuron, astrocyte and oligodendrocyte. RA can induce hUCMs to differentiate into neuron and oligodendrocyte while for astrocyte differentiation DMSO had a pivotal role.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Neural differentiation of human umbilical cord matrix-derived mesenchymal cells under special culture conditions

et al. 2014

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“…Based on their various advantages, the use of hUC-MSCs has yielded good results in the treatment of cardiovascular diseases (Wu et al, 2007;Latifpour et al, 2011). Although the treatment of MSCs has progressed in recent years, their effects of ischemia, hypoxia, invasion of inflammatory cells, and other factors in damaged zones can lead to oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Adaptive protection against damage of preconditioning human umbilical cord-derived mesenchymal stem cells with hydrogen peroxide

Li¹,

Xu²,

Gao³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Adaptive protection against damage to human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) was investigated by preconditioning with low-concentration hydrogen peroxide (H 2 O 2 ) for a short-time period. Separation, culture, amplification, purification, and identification of immunophenotype and growth curve measurements of hUC-MSCs were performed in vitro. At the logarithmic phase, hUCMSCs were incubated with different H 2 O 2 concentrations for 1 and 12 h, and the effects were detected by a cell metabolism assay. Then, hUCMSCs were preconditioned with 10, 20, 50, and 100 mM H 2 O 2 for 1 h, restored for 0, 12, and 24 h, and then damaged with 700, 800, and 900 mM H 2 O 2 for 12 h. Cell morphology, cell metabolism, and the number of cells were measured to determine the protective role of preconditioning. Flow cytometry analysis revealed that the cells expressed CD29 and CD44, but not CD34 and CD45. The growth curve showed that hUCMSCs reached the logarithmic phase in 3-6 days. The cell metabolism assay showed that H 2 O 2 induced hUC-MSCs damage in a dose-and time-dependent manner. The cell morphology, cell metabolism, and number of cells all showed that preconditioning with 10, 20, 50, and 100 7305Adaptive protection of hydrogen peroxide

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“…Our group has developed a standard procedure to acquire hUC-MSCs and established the first hUC-MSCs bank in China. Our intensive studies on the possible clinical application of this type of cells suggest that they can work as a promising candidate for cell-based therapy because of their young age and low infection rate compared to MSCs in adult tissues [6][7][8][9]. A large population of hUC-MSCs sustained in high viability condition is fundamental to successful cell-based therapy.…”

Section: Detecting Viability Transitions Of Umbilical Cord Mesenchymamentioning

confidence: 99%

Raman Spectroscopy for Noninvasive Monitoring of Umbilical Cord Mesenchymal Stem Cells Viability Transitions

P.Chen¹,

F.Zhang²,

Lin³

et al. 2011

Stem Cells in Clinic and Research

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Human umbilical cord derived stem cells for the injured heart

Cited by 16 publications

References 9 publications

Neural differentiation of human umbilical cord matrix-derived mesenchymal cells under special culture conditions

Neural differentiation of human umbilical cord matrix-derived mesenchymal cells under special culture conditions

Adaptive protection against damage of preconditioning human umbilical cord-derived mesenchymal stem cells with hydrogen peroxide

Raman Spectroscopy for Noninvasive Monitoring of Umbilical Cord Mesenchymal Stem Cells Viability Transitions

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