Human upper epidermal cytoplasmic antibodies are directed against keratin intermediate filament proteins.

Hintner, Helmut; Steinert, Peter M.; Lawley, Thomas J.

doi:10.1172/jci111090

Cited by 47 publications

(11 citation statements)

References 31 publications

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“…8,9 Nearly all individuals may possess antibodies against keratin in¬ termediate filaments, and patients with some skin dis¬ eases seem to have higher titers.9 These antibodies are directed against one or several keratin proteins of high molecular weight ranging from 50 to 67 kd. Circulating antibodies to cytoskeletal keratins of epithelial rat cells have been reported to occur in 50% of patients with SLE.…”

Section: Immunoblot Resultsmentioning

confidence: 99%

Association of Tissue-Fixed Cytoplasmic Deposits of Immunoglobulin in Epidermal Keratinocytes With Lupus Erythematosus

Ioannides

Bystryn²

1993

Arch Dermatol

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Section: Immunoblot Resultsmentioning

confidence: 99%

Association of Tissue-Fixed Cytoplasmic Deposits of Immunoglobulin in Epidermal Keratinocytes With Lupus Erythematosus

Ioannides

Bystryn²

1993

Arch Dermatol

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“…In the last 10 yr antibodies against selfcomponents have been described, although at relatively low concentrations, in sera of apparently healthy animals and humans (3,(31)(32)(33) proteins of the keratin (34,35) and the vimentin (36) types, actin, tubulin, and spectrin (e.g., references 1, 2, and 37) and a variety of soluble cytoplasmic and secretory proteins such as thyroglobulin, myoglobin, albumin, and transferrin (33). Most of these natural autoantibodies examined have been found to belong to the IgM class (1,3,36,38).…”

Section: Resultsmentioning

confidence: 99%

High concentrations of antibodies to xanthine oxidase in human and animal sera. Molecular characterization.

Bruder¹,

Jarasch²,

Heid³

1984

J. Clin. Invest.

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Abstract. The widespread occurrence of antibodies (IgG) specific to xanthine oxidase in both normal (nonimmune) human and animal sera, and in antisera raised against a diversity of unrelated antigens is described. A study of sera from 81 humans revealed that xanthine oxidase-specific IgG represents a high proportion (1-8%) of total IgG. No obvious correlation to pathological events or symptoms of disease could be found. These xanthine oxidase-specific antibodies could be isolated by immunoaffinity chromatography on purified human or bovine xanthine oxidase and showed specific binding to the enzyme polypeptide of M, 155,000 in immunoblotting experiments. By immunofluorescence microscopy they displayed the same cell type-specific reaction as experimentally induced antibodies, i.e., the staining of lactating mammary gland epithelium and capillary endothelium.The naturally occurring xanthine oxidase-specific antibodies consisted of polyclonal IgG of various subclasses. F(ab')2 preparations gave immune-reactions identical. to those of IgG. The human xanthine oxidasespecific IgG cross-reacted with the bovine enzyme and both human and animal antibodies partially inhibited its activity. The xanthine oxidase activity of human milk lipid globules and supernatant fractions from various human tissues was extremely low when compared with that of the bovine antigen. The enzyme protein, however, was effectively precipitated from these sources by both the human and bovine antibodies.We suggest that the exceptionally high concentrations

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“…4a), the latter identified by comigrating desmoplakin I with a mouse anticow desmoplakin I antibody (Boehringer Mannheim, Vienna, Austria) (Fig. 12 Immunoprecipitation with patient serum using a mouse monoclonal antiplakoglobin antibody comigrating (Progen, Heidelberg, Germany) also showed a weak 85 kDa band (Fig. However, a 50±65 kDa band was also observed, probably corresponding to different types of keratin as antigens.…”

Section: Immunoblotting and Immunoprecipitationmentioning

confidence: 98%

A localized variant of paraneoplastic pemphigus: acantholysis associated with malignant melanoma

et al. 2001

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We report a 72-year-old male patient with a nodular malignant melanoma that was associated with focal suprabasal acantholysis (FSA). This phenomenon, which is regarded as an incidental finding by dermatopathologists, may be associated with inflammatory and also neoplastic skin diseases. Haematoxylin and eosin stained sections from an erythematous plaque surrounding the patient's tumour showed FSA, direct immunofluorescence (DIF) and indirect immunofluorescence (IIF) on normal human skin, monkey oesophagus and rat urinary bladder were negative. On electron microscopy few desmosomes could be detected in the basal cell layer of the acantholytic areas and there was a nearly complete loss of these structures in the spinous cell layer. Only remnants of cytoplasmic plaques and keratin filaments could be observed in those areas. In contrast, adherens junctions appeared to be well preserved. An enzyme-linked immunosorbent assay (ELISA) using recombinant fusion proteins as antigens did not show circulating autoantibodies against desmoglein 1 (Dsg1) or desmoglein 3 (Dsg3). In contrast, immunoblotting revealed autoantibodies directed against keratinocyte antigens with a molecular weight of 85 kDa and 250 kDa, the first band corresponding to the molecular weight of comigrating plakoglobin. Immunoprecipitation with patient serum also revealed a 85-kDa band. We conclude that these autoantibodies, probably in conjunction with cofactors produced by the tumour, could play a part in the pathogenesis of this variant of FSA, for which we propose the term 'localized paraneoplastic pemphigus.'

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Human upper epidermal cytoplasmic antibodies are directed against keratin intermediate filament proteins.

Cited by 47 publications

References 31 publications

Association of Tissue-Fixed Cytoplasmic Deposits of Immunoglobulin in Epidermal Keratinocytes With Lupus Erythematosus

Association of Tissue-Fixed Cytoplasmic Deposits of Immunoglobulin in Epidermal Keratinocytes With Lupus Erythematosus

High concentrations of antibodies to xanthine oxidase in human and animal sera. Molecular characterization.

A localized variant of paraneoplastic pemphigus: acantholysis associated with malignant melanoma

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