Human Wapl Is a Cohesin-Binding Protein that Promotes Sister-Chromatid Resolution in Mitotic Prophase

Gandhi, Rita; Gillespie, Peter J.; Hirano, Takeshi

doi:10.1016/j.cub.2006.10.061

Cited by 405 publications

(491 citation statements)

References 34 publications

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“…Early studies had suggested that direct phosphorylation of cohesin subunits by Plk1 might promote release of cohesin from chromosome arms (Losada et al 2002;Sumara et al 2002). However, this process turned out to be much more complex, requiring a specialized releasing factor, Wapl (Gandhi et al 2006;Kueng et al 2006), and its partner, Pds5 (Shintomi and Hirano 2009).…”

Section: Prophase Condensation Mediated By Condensin II Coincides Witmentioning

confidence: 99%

“…The interaction of Wapl-Pds5 with the cohesin complex is multilayered and highly complex: Wapl, for instance, interacts not only with its regulatory subunits (SA1-Rad21/Scc1) (Gandhi et al 2006;Shintomi and Hirano 2009), but also with the SMC3 head domain (Chatterjee et al 2013). It will be of great interest and importance in the future to understand why HEAT repeats are found in many components of the cohesion and condensation machinery (Fig.…”

Section: Prophase Condensation Mediated By Condensin II Coincides Witmentioning

confidence: 99%

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Chromosome Dynamics during Mitosis

Hirano

2015

Cold Spring Harb Perspect Biol

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The primary goal of mitosis is to partition duplicated chromosomes into daughter cells. Eukaryotic chromosomes are equipped with two distinct classes of intrinsic machineries, cohesin and condensins, that ensure their faithful segregation during mitosis. Cohesin holds sister chromatids together immediately after their synthesis during S phase until the establishment of bipolar attachments to the mitotic spindle in metaphase. Condensins, on the other hand, attempt to "resolve" sister chromatids by counteracting cohesin. The products of the balancing acts of cohesin and condensins are metaphase chromosomes, in which two rod-shaped chromatids are connected primarily at the centromere. In anaphase, this connection is released by the action of separase that proteolytically cleaves the remaining population of cohesin. Recent studies uncover how this series of events might be mechanistically coupled with each other and intricately regulated by a number of regulatory factors.

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Section: Prophase Condensation Mediated By Condensin II Coincides Witmentioning

confidence: 99%

Section: Prophase Condensation Mediated By Condensin II Coincides Witmentioning

confidence: 99%

Chromosome Dynamics during Mitosis

Hirano

2015

Cold Spring Harb Perspect Biol

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“…This reduced transmission is very sensitive to changes in the dosage of genes involved in chromosome segregation (Dobie et al 2001). For example, heterozygous wings-apart-like (wapl) mutations, which decrease mitotic chromatid separation by reducing prophase removal of cohesin from chromosome arms, decrease J21A transmission in female meiosis (Dobie et al 2001;Gandhi et al 2006;Kueng et al 2006;Verni et al 2000). Females heterozygous for the parental chromosome in which the Nipped-B 407 and Nipped-B 292.1 mutations were induced (Rollins et al 1999) transmitted J21A at a frequency of 0.24 (348 of 1,441; Table 2).…”

Section: Early Sc Breakdown In Nipped-b Mutants Does Not Affect Meiotmentioning

confidence: 99%

Functional links between Drosophila Nipped-B and cohesin in somatic and meiotic cells

et al. 2007

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Drosophila Nipped-B is an essential protein that has multiple functions. It facilitates expression of homeobox genes and is also required for sister chromatid cohesion. Nipped-B is conserved from yeast to man, and its orthologs also play roles in deoxyribonucleic acid repair and meiosis. Mutation of the human ortholog, Nipped-B-Like (NIPBL), causes Cornelia de Lange syndrome (CdLS), associated with multiple developmental defects. The Nipped-B protein family is required for the cohesin complex that mediates sister chromatid cohesion to bind to chromosomes. A key question, therefore, is whether the Nipped-B family regulates gene expression, meiosis, and development by controlling cohesin. To gain insights into Nipped-B's functions, we compared the effects of several Nipped-B mutations on gene expression, sister chromatid cohesion, and meiosis. We also examined association of Nipped-B and cohesin with somatic and meiotic chromosomes by immunostaining. Missense Nipped-B alleles affecting the same HEAT repeat motifs as CdLS-causing NIPBL mutations have intermediate effects on both gene expression and mitotic chromatid cohesion, linking these two functions and the role of NIPBL in human development. Nipped-B colocalizes extensively with cohesin on chromosomes in both somatic and meiotic cells and is present in soluble complexes

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“…This population is essential in preventing precocious separation of the sister chromatids and allowing proper chromosome alignment (Vass et al 2003;Vagnarelli et al 2004;. The dissociation of cohesin in prophase requires Polo and Aurora B kinases as well as a number of additional factors that include Wapl Sumara et al 2002;Hauf et al 2005;Gandhi et al 2006;Kueng et al 2006). At the onset of anaphase, cleavage of Scc1/Rad21 by separase drives dissociation of the remaining cohesin and sister chromatid separation ensues (Hauf et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Shugoshin regulates cohesion by driving relocalization of PP2A in Xenopus extracts

Rivera

Losada

2008

Chromosoma

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Human Wapl Is a Cohesin-Binding Protein that Promotes Sister-Chromatid Resolution in Mitotic Prophase

Abstract: Wapl is a new regulator of sister chromatid resolution and promotes release of cohesin from chromosomes by directly interacting with its regulatory subunits.

Cited by 405 publications

References 34 publications

Chromosome Dynamics during Mitosis

Chromosome Dynamics during Mitosis

Functional links between Drosophila Nipped-B and cohesin in somatic and meiotic cells

Shugoshin regulates cohesion by driving relocalization of PP2A in Xenopus extracts

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