Human Wharton’s Jelly Mesenchymal Stem Cells Protect Neural Cells from Oxidative Stress Through Paracrine Mechanisms

Puig‐Pijuan, Teresa; Godoy, Mateus G.; Carvalho, Luiza; Bodart‐Santos, Victor; Lindoso, Rafael Soares; Pimentel‐Coelho, Pedro M.; Mendez-Otero, Rosália

doi:10.2144/fsoa-2020-0036

Cited by 16 publications

(14 citation statements)

References 58 publications

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“…administered MSC that reach the brain is very low and insufficient to explain their therapeutic effects [ 11 , 26 , 27 ]. Although the mechanisms of action of MSC are not fully understood, it has been shown that MSC can release extracellular vesicles that could reach injured tissues, and the anti-inflammatory effects of MSC in the spleen are widely recognized [ 21 , 23 , 28 ]. Recent studies have also found that the majority of iv.…”

Section: Resultsmentioning

confidence: 99%

Hyperacute Transplantation of Umbilical Cord Mesenchymal Stromal Cells in a Model of Severe Intracerebral Hemorrhage

et al. 2022

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Aim: Intracerebral hemorrhage (ICH) has limited therapeutic options. We have shown that an intravenous injection of human umbilical cord-derived mesenchymal stromal cells (hUC-MSC) 24 h after an ICH in rats reduced the residual hematoma volume after a moderate hemorrhage but was inefficient in severe ICH. Here, we investigated whether a treatment in the hyperacute phase would be more effective in severe ICH. Materials & methods: Wistar rats were randomly selected to receive an intravenous injection of hUC-MSC or the vehicle 1 h after a severe ICH. Results: The hyperacute treatment with hUC-MSC did not affect the 22-day survival rate, the motor function or the residual hematoma volume. Conclusion: These results indicate the need for optimization of hUC-MSC-based therapies for severe ICH.

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Section: Resultsmentioning

confidence: 99%

Hyperacute Transplantation of Umbilical Cord Mesenchymal Stromal Cells in a Model of Severe Intracerebral Hemorrhage

et al. 2022

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“…The BMSCs derived exosomes have some antioxidant components that might have protected the cells in the oxidative stress environment. Recently it has been shown that mesenchymal stem cells derived exosomes have neuroprotective and antioxidant effects on neural cells against oxidative stress generated by different molecules such as hydrogen peroxide and amyloid β-peptide [ 51 , 52 ]. MSCs derived exosomes contain active catalase for combating oxidative stress having profound therapeutic effect on neurodegenerative diseases [ 52 ].…”

Section: Resultsmentioning

confidence: 99%

Transplantation of engineered exosomes derived from bone marrow mesenchymal stromal cells ameliorate diabetic peripheral neuropathy under electrical stimulation

Singh

Raghav

Shiekh

et al. 2021

Bioactive Materials

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Diabetic peripheral neuropathy (DPN) is a long-term complication associated with nerve dysfunction and uncontrolled hyperglycemia. In spite of new drug discoveries, development of effective therapy is much needed to cure DPN. Here, we have developed a combinatorial approach to provide biochemical and electrical cues, considered to be important for nerve regeneration. Exosomes derived from bone marrow mesenchymal stromal cells (BMSCs) were fused with polypyrrole nanoparticles (PpyNps) containing liposomes to deliver both the cues in a single delivery vehicle. We developed DPN rat model and injected intramuscularly the fused exosomal system to understand its long-term therapeutic effect. We found that the fused system along with electrical stimulation normalized the nerve conduction velocity (57.60 ± 0.45 m/s) and compound muscle action potential (16.96 ± 0.73 mV) similar to healthy control (58.53 ± 1.10 m/s; 18.19 ± 1.45 mV). Gastrocnemius muscle morphology, muscle mass, and integrity were recovered after treatment. Interestingly, we also observed paracrine effect of delivered exosomes in controlling hyperglycemia and loss in body weight and also showed attenuation of damage to the tissues such as the pancreas, kidney, and liver. This work provides a promising effective treatment and also contribute cutting edge therapeutic approach for the treatment of DPN.

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“…A similar neuroprotective effect independent of the oxygen concentration was also observed by Lech et al [ 81 ] for hWJ-MSCs cultured in 3D hydrogel scaffolds. Puig-Pijuan et al also proved potential neuroprotective and antioxidants effects of WJ-MSCs on hippocampal cultures [ 86 ].…”

Section: Discussionmentioning

confidence: 99%

Assessment of the Neuroprotective and Stemness Properties of Human Wharton’s Jelly-Derived Mesenchymal Stem Cells under Variable (5% vs. 21%) Aerobic Conditions

Tomecka

Lech

Zychowicz

et al. 2021

Cells

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To optimise the culture conditions for human Wharton’s jelly-derived mesenchymal stem cells (hWJ-MSCs) intended for clinical use, we investigated ten different properties of these cells cultured under 21% (atmospheric) and 5% (physiological normoxia) oxygen concentrations. The obtained results indicate that 5% O2 has beneficial effects on the proliferation rate, clonogenicity, and slowdown of senescence of hWJ-MSCs; however, the oxygen level did not have an influence on the cell morphology, immunophenotype, or neuroprotective effect of the hWJ-MSCs. Nonetheless, the potential to differentiate into adipocytes, osteocytes, and chondrocytes was comparable under both oxygen conditions. However, spontaneous differentiation of hWJ-MSCs into neuronal lineages was observed and enhanced under atmospheric oxygen conditions. The cells relied more on mitochondrial respiration than glycolysis, regardless of the oxygen conditions. Based on these results, we can conclude that hWJ-MSCs could be effectively cultured and prepared under both oxygen conditions for cell-based therapy. However, the 5% oxygen level seemed to create a more balanced and appropriate environment for hWJ-MSCs.

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Human Wharton’s Jelly Mesenchymal Stem Cells Protect Neural Cells from Oxidative Stress Through Paracrine Mechanisms

Cited by 16 publications

References 58 publications

Hyperacute Transplantation of Umbilical Cord Mesenchymal Stromal Cells in a Model of Severe Intracerebral Hemorrhage

Hyperacute Transplantation of Umbilical Cord Mesenchymal Stromal Cells in a Model of Severe Intracerebral Hemorrhage

Transplantation of engineered exosomes derived from bone marrow mesenchymal stromal cells ameliorate diabetic peripheral neuropathy under electrical stimulation

Assessment of the Neuroprotective and Stemness Properties of Human Wharton’s Jelly-Derived Mesenchymal Stem Cells under Variable (5% vs. 21%) Aerobic Conditions

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