Humatrix, a novel myoepithelial matrical gel with unique biochemical and biological properties

PAR2-dependent activation of GSK3β regulates the survival of colon stem/progenitor cells

“…In 3D culture, the PAR 2 -GSK3␤ pathway was constitutively activated. This could occur either through autocrine activation linked to cell adhesion (8,16) or Matrigel-bound proteases (28). The perinuclear localization of PAR 2 that we show in immunostaining experiments could result from constitutive internalization or from a reserve pool in the Golgi, suggesting that PAR 2 is in permanence activated and then degraded and must be synthesized de novo (2).…”

Matrigel coated polydimethylsiloxane based microfluidic devices for studying metastatic and non-metastatic cancer cell invasion and migration

“…In addition, the coating technique can be tailored to deposit different composition of extracellular matrix components like Collagen type I (Schor et al 1982) or Humatrix (Kedeshian et al 1998) with different thickness and length corresponding to different micro-environment studies. Multiple layers of such components can also be deposited to mimic biophysical barriers like the basement membrane and interstitial tissue stroma.…”

Myogel, a Novel, Basement Membrane-Rich, Extracellular Matrix Derived from Skeletal Muscle, Is Highly Adipogenic in vivo and in vitro

“…Other substances formulated as hydrogels include chitosan from crustacean shells, fibrin (a blood-clotting polymer), alginate extracted from seaweed [Eiselt et al, 2000;Kuo and Ma, 2001;Chen et al, 2003;Wang et al, 2003] and Puramatrix, a synthetic polypeptide hydrogel [Bokhari et al, 2005]. Hydrogels prepared from tissue extracts include Amgel [Siegal et al, 1993], Humatrix [Kedeshian et al, 1998] and Matrigel [Kleinman et al, 1982[Kleinman et al, , 1986Kleinman and Martin, 2005]. Small intestinal submucosa, an ECM scaffold containing endogenous growth factors, has been used for several other tissue engineering applications [Hoddle, 2002;Cimini et al, 2005;Zhang et al, 2005] and may also be suitable for adipose engineering, as adipose tissue has grown into the scaffold in other applications [Badylak et al, 2003].…”