Humidity Detection and Hygropreference Behavior in Larvae of the Tobacco hornworm,<i>Manduca sexta</i>

Rowley, Marc; Hanson, Frederick W.

doi:10.1673/031.007.3901

Cited by 15 publications

(13 citation statements)

References 19 publications

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“…pipiens, in addition to the insulating effects of cuticular hydrocarbons and lower metabolic rate that protect diapausing females (Benoit and Denlinger 2007), they can further succeed in reducing water loss and increase longetivity through the fine-tuning of their thermohygroreceptors (Kellog 1970, Davis andSokolove 1975) that serve to guide them to zones with a cooler microclimate with air at lower saturation deficit levels. Hygroreceptive neurons assist desiccated larvae of the tobacco hornworm, Manduca sexta, in finding water (Rowley and Hanson 2007).…”

Section: Discussionmentioning

confidence: 99%

Responses of Anopheles gambiae, Anopheles stephensi, Aedes aegypti, and Culex pipiens mosquitoes (Diptera: Culicidae) to cool and humid refugium conditions

Kessler¹,

Guérin²

2008

Journal of Vector Ecology

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Like all terrestrial arthropods, mosquitoes must cope with the threat of desiccation. To gain insight into their survival strategies, we recorded the behavioral responses of Anopheles gambiae, Anopheles stephensi, Aedes aegypti, and Culex pipiens offered zones of different microclimatic conditions in laboratory cages. The cooled refugium was at 25.6° C, 86% RH and the control was at 28.5° C, 75% RH, i.e., a difference in saturation deficit of 3.9 mm Hg between the two zones. We show that newly-emerged adults, with no access to water or sugar, prefer the cooler and more humid refugium with a saturation deficit half that in the control and where the mosquitoes could reduce their metabolic rate. This response is delayed in Ae. aegypti, perhaps because the energy reserves accumulated as larvae are higher in this species. This study shows that mosquitoes under stress can use their thermohygroreceptor cells to guide them to locations that facilitate survival.

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Section: Discussionmentioning

confidence: 99%

Responses of Anopheles gambiae, Anopheles stephensi, Aedes aegypti, and Culex pipiens mosquitoes (Diptera: Culicidae) to cool and humid refugium conditions

Kessler¹,

Guérin²

2008

Journal of Vector Ecology

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“…Subsequent replay of the images permitted visual evaluation of area consumed. Observer error was estimated at ± 5%; this was determined with spot checks by another observer and using area‐measuring software written in our laboratory specifically for disc tests (Rowley et al., ; Rowley & Hanson, ). For the first 50 min of each experiment when rapid changes in consumption were occurring, all images were evaluated.…”

Section: Methodsmentioning

confidence: 99%

Innate and acquired components of oligophagy in the herbivorous lepidopteran, Manduca sexta

Gretes

Stanwyck

Hanson

2016

Entomologia Exp Applicata

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Innate and acquired aspects of oligophagy were investigated in the tobacco hornworm, Manduca sexta L. (Lepidoptera: Sphingidae), a species normally restricted to members of the family Solanaceae. Larvae were reared in the laboratory on solanaceous species tobacco (Nicotiana tabacum L.), strawberry groundcherry (Physalis pruinosa L.), and potato (Solanum tuberosum L.), as well as the nonsolanaceous foods cowpea [Vigna sinensis (L.) (Fabaceae)], rapeseed [Brassica napus L. (Brassicaceae)], and artificial diet. Feeding assays were conducted using the above plants plus corkscrew vine [Vigna caracalla (L.) Verdc. (Fabaceae)], lettuce [Lactuca sativa L. (Asteraceae)], and moist glassfiber filter paper. Larval feeding was characterized using two camera-monitored assays: (1) a quantitative no-choice disc test that determines consumption over time, delays in initiation of feeding, and % of larvae rejecting the food, and (2) a novel no-choice emigration test that measures how soon a larva abandons a whole plant. Experimental results from both assays revealed that larvae displayed high levels of acceptance of Solanaceae regardless of whether they had been reared on solanaceous or non-solanaceous food. We conclude that solanaceous oligophagy in M. sexta is primarily innate and does not require (but may be strengthened by) previous feeding experience on Solanaceae. In contrast, larvae tested on non-solanaceous plants or moist filter paper showed large variation in both acceptance of foods and emigration times that were strongly dependent on the food on which they had been reared (analogous to the 'induction of preference' of earlier literature). Two types of induction were identified: an increase in acceptability of a plant of (1) the same species as the one on which the larva was reared, or (2) a related species. These discoveries both challenge and expand our current understanding of oligophagy in the tobacco hornworm.

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“…The ability to sense water in the environment, hygrosensation, has been previously studied in a number of insect species [1][2][3][4]. The availability of water impacts insect longevity, fitness and geographic distribution [6].…”

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Aedes aegypti Ir8a mutant female mosquitoes show increased attraction to standing water

Raji

González

DeGennaro

2019

Communicative & Integrative Biology

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The detection of water sources is crucial for insects such as mosquitoes to avoid desiccation and survive. In addition, mosquitoes use humidity cues to successfully navigate the environment to find a suitable oviposition site. Previous studies have implicated some members of the ionotropic receptor family in humidity sensing by Drosophila. Here, we investigate if IR8a co-receptor mediates water detection in Aedes aegypti mosquitoes. Using a simple behavioral assay, we examined the attraction of Ir8a mutant mosquitoes to standing water. Ir8a mutant mosquitoes were able to discriminate between traps containing water and those without as well as wild-type and heterozygous control females. Surprisingly, the female mutants were more robustly drawn to standing water than control mosquitoes. Further investigation revealed that the increased behavioral attraction to water is likely not mediated by a metabolic need or an activity defect. ARTICLE HISTORY

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Humidity Detection and Hygropreference Behavior in Larvae of the Tobacco hornworm,Manduca sexta

Cited by 15 publications

References 19 publications

Responses of Anopheles gambiae, Anopheles stephensi, Aedes aegypti, and Culex pipiens mosquitoes (Diptera: Culicidae) to cool and humid refugium conditions

Responses of Anopheles gambiae, Anopheles stephensi, Aedes aegypti, and Culex pipiens mosquitoes (Diptera: Culicidae) to cool and humid refugium conditions

Innate and acquired components of oligophagy in the herbivorous lepidopteran, Manduca sexta

Aedes aegypti Ir8a mutant female mosquitoes show increased attraction to standing water

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