Humoral Factor in Irradiation Protection: Modification of Lethal Irradiation Injury in Mice by Injection of Rat Bone Marrow

The Use of Regenerating Bone Marrow to Protect Guinea-Pigs against Lethal Irradiation

“…When irradiated animals are transfused with normal bone marrow their lymphoid tissue also recovers (15,42) and it is colonised by cells from the donor marrow (22). Its recovery is slower than that of bone marrow (37) but it may be speeded by injecting splenic cells (16).…”

Cross-species bone marrow transplantation: evidence for tolerance induction, stem cell engraftment, and maturation of T lymphocytes in a xenogeneic stromal environment (rat----mouse).

“…It has long been known that rat bone marrow injected into irradiated mice can significantly prolong survival (6)(7)(8)(9) . Congdon and Lorenz (1954) reported that survival of irradiated recipient mice was prolonged to 21 days following the single intravenous injection of rat bone marrow cells (6) . Using a phosphatase stain for leukocytes, Nowell et al .…”

“…extend this work to demonstrate that the transplanted bone marrow was probably functioning hematopoietically as evidenced by rat leukocyte production (7) . However, longitudinal studies were limited by poor survival of the chimeras, with most chimeras surviving less than 4 weeks following reconstitution (6,7) . Mice which survived long term had erythrocytes and granulocytes of rat origin detectable (7) .…”

Introduction to a review series on advances in cell-based immune therapeutics in hematology

“…The hormone theory was only put to rest when Lorenz and others showed that repopulating marrow bore the same unique chromosomal marker of the infused cells. [2][3][4] This observation established the potential of marrow cells to engraft in a recipient and led to an explosion of laboratory experiments in the 1950s discovering and characterizing all the major phenomena of stem cell transplantation: graftversus-host disease (GVHD), rejection, and the graft-versus-leukemia effect. 5 Furthermore, physicians were not slow to put the new concept of marrow transplantation to clinical use, in autologous transplantation to mitigate the effect of supralethal total body irradiation for cancer and leukemia, and by the late 1960s, the successful application of HLA typing to perform matched allogeneic marrow transplants for immune deficiencies, aplastic anemia, and leukemia.…”