Humulus lupulus L. (Hop): In Vitro Culture; Attempted Production of Bittering Components and Novel Disease Resistance

Heale, J. B.; Legg, T.; Connell, Sara A.

doi:10.1007/978-3-642-73617-9_15

Cited by 11 publications

(11 citation statements)

References 20 publications

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“…There have been a number of in vitro studies on hop, including meristem culture for virus elimination (Adams, 1975), callus establishment (Langezaal and Scheffer, 1992) and regeneration (Heale et al, 1989). In recent years, thidiazuron (N-phenyl-N H -1,2,3-thidiazol-5-ylurea [TDZ]) has emerged as an efficient plant growth regulator (PGR) that enhances morphogenesis in a number of plants (Murthy et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Development of a shoot multiplication system for hop (Humulus lupulus L.)

Roy

Leggett²,

Koutoulis

2001

In Vitro Cell.Dev.Biol.-Plant

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Nodal explants from hop were exposed to plant growth regulators to determine suitable media for initiation from axillary buds and subsequent micropropagation. Efficient culture establishment (96.6% of explants) was achieved on Murashige and Skoog (MS) medium (modified to contain 1 mg l 2l thiamine hydrochloride) supplemented with 0.57 mM indoleacetic acid (IAA) and 2.22 mM 6-benzylaminopurine (BA). Subsequent transfer of explants to treatments containing an auxin ([1-naphthaleneacetic acid] NAA or IAA) and BA, 6-[g,g-dimethylallylamino]purine (2iP), kinetin (KIN) or thidiazuron (N-phenyl-N H -1,2,3-thidiazol-5-ylurea [TDZ]) resulted in significantly different amounts of multiplication. Optimal TDZ-supplemented media elicited a greater than threefold increase in the number of shoots and nodes generated per explant compared to optimal media containing BA, 2iP and KIN. Shoots were successfully rooted using half-strength MS supplemented with 5.71 mM IAA and 4.9 mM indolebutyric acid (IBA), and regenerated plants were successfully transferred to soil. An overall micropropagation schedule, which can be implemented into hop commercialization programs, includes: (i) establishment in MS with 0.57 mM IAA and 2.22 mM BA; (ii) multiplication in MS with 0.57 mM IAA and 2.27 mM TDZ; (iii) elongation in MS; and (iv) rooting in half-strength MS with 5.71 mM IAA and 4.9 mM IBA.

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Section: Introductionmentioning

confidence: 99%

Development of a shoot multiplication system for hop (Humulus lupulus L.)

Roy

Leggett²,

Koutoulis

2001

In Vitro Cell.Dev.Biol.-Plant

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“…1, 2) for use in Agrobacterium-mediated transformation with overall regeneration frequencies higher than 40%. In his work on hop regeneration, Becker (2000) compared several media previously reported (Motegi 1979;Heale et al 1989;Rakousky and MatouÐek 1994;Batista et al 1996;Oriniakovµ and MatouÐek 1996). He introduced TDZ (Huetteman and Preece 1993; Murthy et al 1998) as growth regulator, which had been proven to be very suitable for promoting the regeneration of hop plants.…”

Section: Establishment Of An Efficient Regeneration Systemmentioning

confidence: 95%

“…Various reports have been published on regeneration of hop meristems for elimination of viruses (Vine and Jones 1969;Gippert et al 1974;Adams 1975;Kubo et al 1975;Samyn and Welvaert 1983;Eppler 1984;Popov et al 1986;Probasco and Winslow 1986;Svoboda 1988Svoboda , 1991Svoboda , 1992Adams et al 1996). However, only few data have become available on the regeneration of tissues other than meristems (Motegi 1976(Motegi , 1979Connell and Heale 1986;Heale et al 1989;Rakousk and MatouÐek 1994;Batista et al 1996;Gurriarµn et al 1999;uÐtar-Volzlič et al 1999;Becker 2000). Becker (2000) and Rakousk and MatouÐek (1994) only reported on direct organogenesis of several explant types.…”

Section: Introductionmentioning

confidence: 94%

Regeneration and Agrobacterium- mediated transformation of hop ( Humulus lupulus L.)

et al. 2003

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An efficient procedure for direct organogenesis and regeneration of hop (Humulus lupulus L.) was established. For the first time Agrobacterium-mediated genetic transformation of hop (cv. "Tettnanger") was achieved. Shoot internodes from in vitro cultures were identified as the most suitable type of explant for regeneration. Using this type of explant, a shoot-inducing medium was developed that supported direct organogenesis of approximately 50% of the explants. Plantlets were successfully rooted and transferred to the greenhouse. Overall, in less than 6 months hop cultures propagated in vitro were regenerated to plants in the greenhouse. Agrobacterium-mediated genetic transformation was performed with the reporter gene GUS (beta-glucuronidase). The presence and function of transgenes in plants growing in the greenhouse was verified by PCR (polymerase chain reaction) and enzyme assay for GUS activity, respectively. We have obtained 21 transgenic plants from 1,440 explants initially transformed, yielding an overall transformation efficiency of 1.5%.

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“…GRIFFIN and COLEY-SMITH (1968) first initiated hop callus cultures with the aim of study in aseptic conditions the infection process of fungus Pseudoperonospora humuli. Callus cultures in hops have been used experimentally to provide a source of regenerationg green nodular callus, for attempted selection of novel disease resistance to Verticillium albo-atrum in somaclonal variants, as well as providing a sterile source of leaf mesophyll protoplasts (CONNELL and HEALE, 1986b;HEALE et al, 1989). There were also attempts to establish regenerating, organogenic callus caltures from different types of explants (MOTEGI, 1976;BATISTA et al, 1996).…”

Section: Callus Culturementioning

confidence: 99%

The use of biotechnology in hop (Humulus lupulus L.) improvement

Faragó

Pšenáková

Faragová

2021

Nova Biotechnol. Chim.

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Hop (Humulus lupulus L.) is a clonally propagated, dioecious, perennial, climbing plant used commercially for their secondary metabolites. The resins containing α- and β-acids, and essential oils produced by the lupulin glands, present on the female flowers are used to add bitterness, aroma and flavour to beer. Recently, flavonoids, including chalcones and flavanones, of hops have been shown to exert a variety of biological activities, including oestrogenic and anticancerogenic characteristics. In this review, we provide a overview of the techniques and opportunities presented by the integration of plant biotechnology into hop improvement. The use of tissue culture techniques such as micropropagation, meristem culture, in vitro storage, adventitious shoot induction, callus culture and cell suspension culture in hops are briefly reviewed. The usefullness of genetic transformation technology to introduce novel traits into hop is also discussed.

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Humulus lupulus L. (Hop): In Vitro Culture; Attempted Production of Bittering Components and Novel Disease Resistance

Cited by 11 publications

References 20 publications

Development of a shoot multiplication system for hop (Humulus lupulus L.)

Development of a shoot multiplication system for hop (Humulus lupulus L.)

Regeneration and Agrobacterium- mediated transformation of hop ( Humulus lupulus L.)

The use of biotechnology in hop (Humulus lupulus L.) improvement

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