Hv-WRKY38: a new transcription factor involved in cold- and drought-response in barley

Marè, Caterina; Mazzucotelli, Elisabetta; Crosatti, Cristina; Francia, Enrico; Stanca, A. M.; Cattivelli, Luigi

doi:10.1007/s11103-004-0906-7

Cited by 276 publications

(185 citation statements)

References 58 publications

(66 reference statements)

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“…In many cases, comparative genomics leads to new findings in agriculturally and economically important crops (Marchive et al 2007;Mare et al 2004;Petitot et al 2013;Shekhawat et al 2011). Recently, Luo et al (2013) have described that in wild soybean, Glycine soja, GswRKY20 promotes the expression of negative regulators of ABA signaling while repressing positive regulators.…”

Section: Abiotic Stress and Wrky Transcription Factors: Altered Exprementioning

confidence: 99%

A systems biology perspective on the role of WRKY transcription factors in drought responses in plants

Tripathi

Rabara

Rushton

2013

Planta

203

130

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Section: Abiotic Stress and Wrky Transcription Factors: Altered Exprementioning

confidence: 99%

A systems biology perspective on the role of WRKY transcription factors in drought responses in plants

Tripathi

Rabara

Rushton

2013

Planta

203

130

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“…HvWRKY1/38 is a key regulator of cold and drought responses, immune response, and seed germination (Marè et al, 2004;Shen et al, 2007;Zou et al, 2008). OsWRKY13 is involved in plant resistance to bacterial infection and responds to drought stress by selectively binding to different target genes (Deng et al, 2012b;Xiao et al, 2013).…”

mentioning

confidence: 99%

Cotton WRKY1 Mediates the Plant Defense-to-Development Transition during Infection of Cotton byVerticillium dahliaeby ActivatingJASMONATE ZIM-DOMAIN1Expression

Luo

et al. 2014

Plant Physiology

138

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Plants have evolved an elaborate signaling network to ensure an appropriate level of immune response to meet the differing demands of developmental processes. Previous research has demonstrated that DELLA proteins physically interact with JASMONATE ZIM-DOMAIN1 (JAZ1) and dynamically regulate the interaction of the gibberellin (GA) and jasmonate (JA) signaling pathways. However, whether and how the JAZ1-DELLA regulatory node is regulated at the transcriptional level in plants under normal growth conditions or during pathogen infection is not known. Here, we demonstrate multiple functions of cotton (Gossypium barbadense) GbWRKY1 in the plant defense response and during development. Although GbWRKY1 expression is induced rapidly by methyl jasmonate and infection by Verticillium dahliae, our results show that GbWRKY1 is a negative regulator of the JA-mediated defense response and plant resistance to the pathogens Botrytis cinerea and V. dahliae. Under normal growth conditions, GbWRKY1-overexpressing lines displayed GA-associated phenotypes, including organ elongation and early flowering, coupled with the down-regulation of the putative targets of DELLA. We show that the GA-related phenotypes of GbWRKY1-overexpressing plants depend on the constitutive expression of Gossypium hirsutum GhJAZ1. We also show that GhJAZ1 can be transactivated by GbWRKY1 through TGAC core sequences, and the adjacent sequences of this binding site are essential for binding specificity and affinity to GbWRKY1, as revealed by dual-luciferase reporter assays and electrophoretic mobility shift assays. In summary, our data suggest that GbWRKY1 is a critical regulator mediating the plant defense-to-development transition during V. dahliae infection by activating JAZ1 expression.

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“…Similarly in barley, expression of HvWRKY38 (a Group II type) was increased during exposure of plants to both cold and drought stresses (Mare et al 2004). On the other hand, little or no expression of CgWRKY1 was detected during cold acclimation in cold-sensitive pummelo plants, while high level of expression was observed in response to drought stress.…”

Section: Discussionmentioning

confidence: 99%

“…Although PtrWRKY1 contains one WRKY domain, it clustered with the N terminal WRKY domain of Group I (1N) WRKY TFs, rather than clustering with Group II types WRKY TFs, which have only one WRKY domain. The phylogenetic tree contains some functionally characterised WRKY TFs, including AtWRKY63, GmWRKY21, GmWRKY54, HvWRKY38, OsWRKY45 and OsWRKY79 that have a role in cold and drought tolerance in different plants (Chen et al 2012;Mare et al 2004;Zhou et al 2008). However, PtrWRKY1 was not phylogenetically closely related to any of these.…”

Section: A Cdna From Poncirus Encodes a Wrky Transcription Factormentioning

confidence: 99%

Identification of a drought- and cold-stress inducibleWRKYgene in the cold-hardyCitrusrelativePoncirus trifoliata

Şahin‐Çevik

Moore

2013

New Zealand Journal of Crop and Horticultural Science

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WRKY transcription factors (TFs) are involved in regulation of abiotic and biotic stress responsive genes and play a role in abiotic and biotic stress tolerance in plants. Although commercial Citrus species are sensitive to cold and drought stresses, an interfertile Citrus relative, Poncirus trifoliata, is resistant to cold and more drought tolerant than most commercial Citrus cultivars. An expressed sequence tag clone sharing homology with WRKY-type TFs was previously isolated from a cDNA library constructed using two-day cold-acclimated P. trifoliata cv. Rubidoux seedlings. In this study, a full-length sequence of this WRKY gene was cloned and isolated from 2-day cold-acclimated P. trifoliata plants using the rapid amplification of cDNA ends (RACE) method. The full-length PtrWRKY1 cDNA is 1807 bp and encodes a polypeptide of 405 amino acids containing a single WRKY domain with a Cys 2 His 2 motif, which is a signature of WRKY TFs. A homologous open reading frame (ORF) sequence was isolated from Citrus grandis, CgWRKY1, sharing 98% identity at the nucleotide level with PtrWRKY1. The expression of PtrWRKY1 and CgWRKY1 was investigated in response to cold and drought stresses by northern blot analysis. While the expression of both genes was induced in response to drought, only PtrWRKY1 expression was induced in response to cold. This differential expression in cold-hardy Poncirus and cold-sensitive pummelo suggests that PtrWRKY1 gene may play a role in adaptation and tolerance to cold stress in Poncirus.

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Hv-WRKY38: a new transcription factor involved in cold- and drought-response in barley

Cited by 276 publications

References 58 publications

A systems biology perspective on the role of WRKY transcription factors in drought responses in plants

A systems biology perspective on the role of WRKY transcription factors in drought responses in plants

Cotton WRKY1 Mediates the Plant Defense-to-Development Transition during Infection of Cotton byVerticillium dahliaeby ActivatingJASMONATE ZIM-DOMAIN1Expression

Identification of a drought- and cold-stress inducibleWRKYgene in the cold-hardyCitrusrelativePoncirus trifoliata

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