Hyaluronan and dextran modified tubes resist cellular activation with blood contact

Eckmann, David M.; Tsai, Irene; Tomczyk, Nancy; Weisel, John W.; Composto, Russell J.

doi:10.1016/j.colsurfb.2013.02.013

Cited by 9 publications

(13 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Advances in medical device surface modification include our group’s recent work on the antibacterial properties and hemocompatibility of grafted surfaces (Coll Ferrer et al, 2013; Dastgheyb et al, 2013; Eckmann et al, 2013; Lee et al, 2013a, 2013b), with many other new developments reviewed by (Campoccia et al, 2013) and (Meyers and Grinstaff, 2012). Emerging technologies in biological research also often require the grafting of biomaterials, including various protein coatings to enable cell and biomolecule attachment in microfluidic devices (Shirtcliffe et al, 2013) and even the immobilization of enzymes for biocatalysis performance (Jia et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Chemically grafted fibronectin for use in QCM-D cell studies

Kandel

Lee

Sobolewski

et al. 2014

Biosensors and Bioelectronics

Self Cite

View full text Add to dashboard Cite

Traditionally, fibronectin has been used as a physisorbed surface coating (physFN) in cell culture experiments due to its critical role in cell adhesion. However, because the resulting layer is thick, unstable, and of unpredictable uniformity, this method of fibronectin deposition is unsuitable for some types of research, including quartz crystal microbalance (QCM) experiments involving cells. Here, we present a new method for chemical immobilization of fibronectin onto silicon oxide surfaces, including QCM crystals pre-coated with silicon oxide. We characterize these chemically coated fibronectin surfaces (chemFN) as well as physFN ones using surface ellipsometry (SE), Fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM), and contact angle measurements. A cell culture model demonstrates that cells on chemFN and physFN surfaces exhibit similar viability, structure, adhesion and metabolism. Finally, we perform QCM experiments using cells on both surfaces which demonstrate the superior suitability of chemFN coatings for QCM research, and provide real-time QCM-D data from cells subjected to an actin depolymerizing agent. Overall, our method of chemical immobilization of fibronectin yields great potential for furthering cellular experiments in which thin, stable and uniform coatings are desirable. As QCM research with cells has been rather limited in success thus far, we anticipate that this new technique will particularly benefit this experimental system by availing it to the much broader field of cell mechanics.

show abstract

Section: Introductionmentioning

confidence: 99%

Chemically grafted fibronectin for use in QCM-D cell studies

Kandel

Lee

Sobolewski

et al. 2014

Biosensors and Bioelectronics

Self Cite

View full text Add to dashboard Cite

show abstract

“…8,9,31 This model enables evaluation of modified surfaces with respect to blood-surface interactions by flowing blood within a circular closed loop of surface modified tubing. Freshly drawn whole human blood, supplemented with sodium citrate, was either kept as control or loaded into PU, PAA/PU, CH/PAA/PU, and CH-Q/PAA/PU tubes (35 cm), connected into loops using 1 cm stainless connectors.…”

Section: Methodsmentioning

confidence: 99%

“…Flow cytometry data collection and analysis was performed with a 4-color FACSCalibur (Becton Dickinson, San Jose, CA) using standard acquisition CellQuest software. 9 …”

Section: Methodsmentioning

confidence: 99%

“…1,16 Generally, the hemocompatibility of a surface is measured by the reduced activation of coagulation, complement and blood cells which result from direct contact of blood with artificial surfaces. 1,2,8,9 Hemocompatibility is also evaluated by cell adhesion resulting from the direct contact of platelets, neutrophils, or whole blood with the modified surfaces. 2-4,8 An example of this is the recent study of Finley et al, which used whole blood adhesion studies and antigen markers for platelets and neutophils, and measured their activation by flow cytomety.…”

Section: Introductionmentioning

confidence: 99%

“…Whole blood adhesion and platelet adhesion tests are performed using CH and CH-Q surfaces in order to study the interrelationship between the plasma protein adsorption and the blood coagulation on the surfaces. To examine the biological response of platelets and neutrophils to biomaterial surfaces, CH and CH-Q grafted layers as well as a PAA grafted layer on PU tubes versus unmodified PU tubing are tested using the Chandler Loop Apparatus 8,9 as an ex vivo model. The tubes are then examined for platelet and neutrophil activation by flow cytometry.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Hemocompatibility of chitosan/poly(acrylic acid) grafted polyurethane tubing

et al. 2013

Self Cite

View full text Add to dashboard Cite

The activation and adhesion of platelets or whole blood exposed to chitosan (CH) grafted surfaces is used to evaluate the hemocompatibility of biomaterials. The biomaterial surfaces are polyurethane (PU) tubes grafted with an inner poly(acrylic acid) (PAA) and an outer CH or quaternary ammonium modified CH (CH-Q) brush. The CH, CH-Q and PAA grafted layers were characterized by ellipsometry and fluorescence microscopy. Material wear tests demonstrate that CH (CH-Q) is stably grafted onto PU tubes upon exposure to saline solution for 7 days. Using quartz-crystal microbalances with dissipation (QCM-D), in-situ adsorption of blood plasma proteins on CH and CH-Q compared to a silicon oxide control was measured. The QCM-D results showed that the physically adsorbed plasma protein layer on CH-Q and CH surfaces is softer and more viscous than the protein layer on the SiO2 surface. The CH-Q layer thus has the weakest interaction with plasma proteins. Whole blood and platelet adhesion was reduced by ~92% on CH-Q, which showed the weakest interaction with plasma protein but more viscous adsorbed plasma protein layer, compared to SiO2. Last, to examine the biologic response of platelets and neutrophils to biomaterial surfaces, CH (CH-Q)/PAA, PAA and PU tubes were tested using a Chandler Loop apparatus as an ex vivo model and flow cytometry. The blood adhesion and biologic response results showed that CH and CH-Q reduced adhesion and activation of platelets and neutrophils and improved hemocompatibility relative to other surfaces (PU and PAA). Our studies demonstrated that the properties of physically adsorbed plasma protein layer on biomaterial surfaces correlates with blood coagulation on biomaterial surfaces.

show abstract

Effect of Recombinant Human Perlecan Domain V Tethering Method on Protein Orientation and Blood Contacting Activity on Polyvinyl Chloride

Chandrasekar

Farrugia

Johnson

et al. 2021

Adv Healthcare Materials

View full text Add to dashboard Cite

Surface modification of biomaterials is a promising approach to control biofunctionality while retaining the bulk biomaterial properties. Perlecan is the major proteoglycan in the vascular basement membrane that supports low levels of platelet adhesion but not activation. Thus, perlecan is a promising bioactive for blood-contacting applications. This study furthers the mechanistic understanding of platelet interactions with perlecan by establishing that platelets utilize domains III and V of the core protein for adhesion. Polyvinyl chloride (PVC) is functionalized with recombinant human perlecan domain V (rDV) to explore the effect of the tethering method on proteoglycan orientation and bioactivity. Tethering of rDV to PVC is achieved via either physisorption or covalent attachment via plasma immersion ion implantation (PIII) treatment. Both methods of rDV tethering reduce platelet adhesion and activation compared to the pristine PVC, however, the mechanisms are unique for each tethering method. Physisorption of rDV on PVC orientates the molecule to hinder access to the integrin-binding region, which inhibits platelet adhesion. In contrast, PIII treatment orientates rDV to allow access to the integrin-binding region, which is rendered antiadhesive to platelets via the glycosaminoglycan (GAG) chain. These effects demonstrate the potential of rDV biofunctionalization to modulate platelet interactions for blood contacting applications.

show abstract

Hyaluronan and dextran modified tubes resist cellular activation with blood contact

Cited by 9 publications

References 46 publications

Chemically grafted fibronectin for use in QCM-D cell studies

Chemically grafted fibronectin for use in QCM-D cell studies

Hemocompatibility of chitosan/poly(acrylic acid) grafted polyurethane tubing

Effect of Recombinant Human Perlecan Domain V Tethering Method on Protein Orientation and Blood Contacting Activity on Polyvinyl Chloride

Contact Info

Product

Resources

About