Hyaluronan/chitosan nanofilms assembled layer-by-layer and their antibacterial effect: A study using Staphylococcus aureus and Pseudomonas aeruginosa

Hernández‐Montelongo, Jacobo; Lucchesi, Eliane Gama; González, Ismael; Macedo, W. A. A.; Nascimento, V. F.; Moraes, Ângela Maria; Beppu, Marisa Masumi; Cotta, M. A.

doi:10.1016/j.colsurfb.2016.02.028

Cited by 54 publications

(34 citation statements)

References 27 publications

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“…[70,79] Besides synthetic polymers, antifouling properties have also been obtained with polysaccharide coatings that decreased bacteria adhesion to a titanium surface up to 96% after 90 min of contact, [50] and agarose crosslinked on a solid support reduced the adhesion of proteins to device surfaces by >90%. [92] The physical property of the coating compound is sometimes coupled to antimicrobial effects from the chemical nature of the polymer; for example, a mixture of hyaluronic acid and chitosan [93] or poly(N-hydroxyethylacrylamide) crosslinked with salicylate [94] decreased bacteria adhesion, and also inhibited their proliferation. [92] The physical property of the coating compound is sometimes coupled to antimicrobial effects from the chemical nature of the polymer; for example, a mixture of hyaluronic acid and chitosan [93] or poly(N-hydroxyethylacrylamide) crosslinked with salicylate [94] decreased bacteria adhesion, and also inhibited their proliferation.…”

Section: Polymer-coated Surfacesmentioning

confidence: 99%

Nanoscience‐Based Strategies to Engineer Antimicrobial Surfaces

et al. 2018

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Microbial contamination and biofilm formation of medical devices is a major issue associated with medical complications and increased costs. Consequently, there is a growing need for novel strategies and exploitation of nanoscience‐based technologies to reduce the interaction of bacteria and microbes with synthetic surfaces. This article focuses on surfaces that are nanostructured, have functional coatings, and generate or release antimicrobial compounds, including “smart surfaces” producing antibiotics on demand. Key requirements for successful antimicrobial surfaces including biocompatibility, mechanical stability, durability, and efficiency are discussed and illustrated with examples of the recent literature. Various nanoscience‐based technologies are described along with new concepts, their advantages, and remaining open questions. Although at an early stage of research, nanoscience‐based strategies for creating antimicrobial surfaces have the advantage of acting at the molecular level, potentially making them more efficient under specific conditions. Moreover, the interface can be fine tuned and specific interactions that depend on the location of the device can be addressed. Finally, remaining important challenges are identified: improvement of the efficacy for long‐term use, extension of the application range to a large spectrum of bacteria, standardized evaluation assays, and combination of passive and active approaches in a single surface to produce multifunctional surfaces.

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Section: Polymer-coated Surfacesmentioning

confidence: 99%

Nanoscience‐Based Strategies to Engineer Antimicrobial Surfaces

et al. 2018

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“…Chitosan is well known in the literature for its numerous biological effects, such as mucoadhesion and permeation enhancement [ 1 , 2 ], antimicrobial activity [ 3 , 4 ], and hemostatic and analgesic action [ 5 ]. It is also endowed with wound healing promotion effect, due to its ability to enhance growth factor and cytokine expression, and to promote the stability of growth factors [ 6 , 7 , 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Association of Alpha Tocopherol and Ag Sulfadiazine Chitosan Oleate Nanocarriers in Bioactive Dressings Supporting Platelet Lysate Application to Skin Wounds

et al. 2018

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Chitosan oleate was previously proposed to encapsulate in nanocarriers some poorly soluble molecules aimed to wound therapy, such as the anti-infective silver sulfadiazine, and the antioxidant α tocopherol. Because nanocarriers need a suitable formulation to be administered to wounds, in the present paper, these previously developed nanocarriers were loaded into freeze dried dressings based on chitosan glutamate. These were proposed as bioactive dressings aimed to support the application to wounds of platelet lysate, a hemoderivative rich in growth factors. The dressings were characterized for hydration capacity, morphological aspect, and rheological and mechanical behavior. Although chitosan oleate nanocarriers clearly decreased the mechanical properties of dressings, these remained compatible with handling and application to wounds. Preliminary studies in vitro on fibroblast cell cultures demonstrated good compatibility of platelet lysate with nanocarriers and bioactive dressings. An in vivo study on a murine wound model showed an accelerating wound healing effect for the bioactive dressing and its suitability as support of the platelet lysate application to wounds.

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“…Rose Bengal (RB, negatively charged) is utilized to detect the amines groups of CHI (positively charged). Similarly, Methylene Blue (MB, positively charged) is used to identify the carboxylic groups of βCD (negatively charged) . In the case of RB detection, the nPSi‐Ox spectrum (control) exhibits a significantly peak, due to its large surface area allows adsorbing the RB molecules.…”

Section: Resultsmentioning

confidence: 99%

Nanoporous Silicon Composite as Potential System for Sustained Delivery of Florfenicol Drug

Hernández‐Montelongo

Oria

Cárdenas

et al. 2018

Physica Status Solidi (b)

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Nanostructured porous silicon (nPSi) is a nanostructured biomaterial which has received considerable attention in biomedical applications due to its biocompatibility, biodegradability, high surface area, and the ease to modify its surface chemistry. In the present work, nPSi composite microparticles are evaluated as potential drug delivery system. nPSi layers are formed by electrochemical etching of silicon wafers in hydrofluoric acid solutions. This fabrication process allows modifying the main properties of nPSi layers, including the porosity, average pore size and pore shape, by simply controlling the main parameters in the process, such as the applied current density and the electrolyte composition. nPSi microparticles are prepared from the removal and fracture by ultrasound sonication of nPSi layers. Composites are obtained from oxidized nPSi (nPSi-Ox) microparticles cascade processed with chitosan (CHI) and β-cyclodextrin (βCD) biopolymers. Samples are evaluated as drug delivery system using florfenicol (FF) as model drug, due to its economical and sanitary importance in salmon industry. Drug loaded and release kinetic tests are performed in different media: distilled water and simulated seawater. Initial data show that nPSi-βCD composites allow a mayor control in the drug time release kinetic compared to nPSi-Ox microparticles.

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Hyaluronan/chitosan nanofilms assembled layer-by-layer and their antibacterial effect: A study using Staphylococcus aureus and Pseudomonas aeruginosa

Cited by 54 publications

References 27 publications

Nanoscience‐Based Strategies to Engineer Antimicrobial Surfaces

Nanoscience‐Based Strategies to Engineer Antimicrobial Surfaces

Association of Alpha Tocopherol and Ag Sulfadiazine Chitosan Oleate Nanocarriers in Bioactive Dressings Supporting Platelet Lysate Application to Skin Wounds

Nanoporous Silicon Composite as Potential System for Sustained Delivery of Florfenicol Drug

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