Hyaluronic acid facilitates chondrogenesis and matrix deposition of human adipose derived mesenchymal stem cells and human chondrocytes co-cultures

Amann, Elisabeth; Wolff, Paul; Breel, Ernst J.; Griensven, Martijn van; Balmayor, Elizabeth R.

doi:10.1016/j.actbio.2017.01.064

Cited by 100 publications

(92 citation statements)

References 58 publications

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“…This biologic action together with the suitable characteristics of the bioscaffold and the HA from the SF may lead to a chondrogenic differentiation of immobilized SF‐MSCs. Other groups also described the potential of combining PRP and HA to induce chondrogenesis, among other benefits, and according with Amman et al our results also corroborated that the formulation which contains the highest HA amount (1:3), expressed more collagen type II than other formulations.…”

Section: Discussionsupporting

confidence: 85%

Autologous bioscaffolds based on different concentrations of platelet rich plasma and synovial fluid as a vehicle for mesenchymal stem cells

Garate¹,

Sánchez

Delgado³

et al. 2017

J Biomedical Materials Res

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In the field of tissue engineering, diverse types of bioscaffolds are being developed currently for osteochondral defect applications. In this work, a novel scaffold based on platelet rich plasma (PRP) and hyaluronic acid with mesenchymal stem cells (MSCs) has been evaluated to observe its effect on immobilized cells. The bioscaffolds were prepared by mixing different volumes of synovial fluid (SF) with PRP from patients obtaining three formulations at PRP-SF ratios of 3:1, 1:1 and 1:3 (v/v). The live/dead staining revealed that although the cell number of each type of bioscaffold was different, these this constructs provide cells with a suitable environment for their viability and proliferation. Moreover, immobilized MSCs showed their ability to secrete fibrinolytic enzymes, which vary depending on the fibrin amount of the scaffold. Immunohistochemical analysis revealed the positive staining for collagen type II in all cases, proving the biologic action of SF derived MSCs together with the suitable characteristics of the bioscaffold for chondrogenic differentiation. Considering all these aspects, this study demonstrates that these cells-based constructs represent an attractive method for cell immobilization, achieving completely autologous and biocompatible scaffolds. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 377-385, 2018.

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Section: Discussionsupporting

confidence: 85%

Autologous bioscaffolds based on different concentrations of platelet rich plasma and synovial fluid as a vehicle for mesenchymal stem cells

Garate¹,

Sánchez

Delgado³

et al. 2017

J Biomedical Materials Res

View full text Add to dashboard Cite

show abstract

“…It is surprising that the addition of HA led to a trend of decreased chondrogenic gene expression, including SOX 9 , COL2 , and AGG , although no significant differences were observed. In fact, a recent study also showed that supplementation with HA leads to a lower expression of collagen type II (Amann, Wolff, Breel, van Griensven, & Balmayor, ). These results disagreed with another recent study, which indicated that higher HA resulted in a higher expression of chondrogenic gene marker genes at Days 1, 4, and 7 (Pfeifer et al, ).…”

Section: Resultsmentioning

confidence: 98%

“…Bar = 100 μm. In (b), no statistical difference was found (n = 4) [Colour figure can be viewed at wileyonlinelibrary.com] FIGURE 3 Expression of chondrogenic (SOX 9, AGG, and COL2) and hypertrophic (COL10) marker genes in cells within different constructs was examined after 8 weeks (n = 4) with HA leads to a lower expression of collagen type II (Amann, Wolff, Breel, van Griensven, & Balmayor, 2017). These results disagreed with another recent study, which indicated that higher HA resulted in a higher expression of chondrogenic gene marker genes at Days 1, 4, and 7 (Pfeifer et al, 2016).…”

Section: Figurementioning

confidence: 99%

Optimization of photocrosslinked gelatin/hyaluronic acid hybrid scaffold for the repair of cartilage defect

Lin

Beck

Shimomura

et al. 2019

J Tissue Eng Regen Med

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Summary There is no therapy currently available for fully repair of articular cartilage lesion. Our laboratory has recently developed a visible light-activatable methacrylated gelatin (mGL) hydrogel with the potential for cartilage regeneration. In this study, we further optimized mGL scaffolds by supplementing methacrylated hyaluronic acid (mHA), which has been shown to stimulate chondrogenesis via activation of critical cellular signaling pathways. We hypothesized that the introduction of an optimal ratio of mHA would enhance the biological properties of mGL scaffolds and augment chondrogenesis of human bone marrow-derived mesenchymal stem cells (hBMSCs). To test this hypothesis, hybrid scaffolds consisting of mGL and mHA at different weight ratios were fabricated with hBMSCs encapsulated at 20×106 cells/mL and maintained in a chondrogenesis-promoting medium. The chondrogenenic differentiation of hBMSCs within different scaffolds was estimated after 8 weeks of culture. Our results showed that mGL/mHA at a 9:1 (%, w/v) ratio resulted in the lowest hBMSC hypertrophy and highest glycosaminoglycan production, with slightly increased volume of the entire construct. The applicability of this optimally designed mGL/mHA hybrid scaffold for cartilage repair was then examined in vivo. A full-thickness cylindrical osteochondral defect was surgically created in the rabbit femoral condyle and a 3-dimensional cell-biomaterial construct was fabricated by in situ photo-crosslinking to fully fill the lesion site. The results showed that implantation of the mGL/mHA (9:1) construct resulted in both cartilage and subchondral bone regeneration after 12 weeks, supporting its use as a promising scaffold for repair and resurfacing of articular cartilage defects in the clinical setting.

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“…44,63,64 Preparation of methacrylated HA and CSC HA and CSC were selected for evaluation based on their structural role in cartilage, their chondroprotective effects, and their ability to facilitate chondrogenesis. 39,[65][66][67][68][69][70] In order to conjugate these GAGs into the PEGDA-based scaffolds, each GAG type was methacrylate-derivatized according to standard protocols. 71 In brief, CSC (51 kDa, 6.4 wt % sulfur, Sigma) and HA (Streptococcus equi, M W~1 .65 × 10 3 kDa, Fluka) were dissolved in deionized (dI)H 2 O to achieve a 1 wt % final concentration, and the pH of each solution was adjusted to 8.0.…”

Section: Methodsmentioning

confidence: 99%

Toward zonally tailored scaffolds for osteochondral differentiation of synovial mesenchymal stem cells

et al. 2018

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Synovium-derived mesenchymal stem cells (SMSCs) are an emerging cell source for regenerative medicine applications, including osteochondral defect (OCD) repair. However, in contrast to bone marrow MSCs, scaffold compositions which promote SMSC chondrogenesis/osteogenesis are still being identified. In the present manuscript, we examine poly(ethylene) glycol (PEG)-based scaffolds containing zonally-specific biochemical cues to guide SMSC osteochondral differentiation. Specifically, SMSCs were encapsulated in PEG-based scaffolds incorporating glycosaminoglycans (hyaluronan or chondroitin-6-sulfate [CSC]), low-dose of chondrogenic and osteogenic growth factors (TGFβ1 and BMP2, respectively), or osteoinductive poly(dimethylsiloxane) (PDMS). Initial studies suggested that PEG-CSC-TGFβ1 scaffolds promoted enhanced SMSC chondrogenic differentiation, as assessed by significant increases in Sox9 and aggrecan. Conversely, PEG-PDMS-BMP2 scaffolds stimulated increased levels of osteoblastic markers with significant mineral deposition. A “Transition” zone formulation was then developed containing a graded mixture of the chondrogenic and osteogenic signals present in the PEG-CSC-TGFβ1 and PEG-PDMS-BMP2 constructs. SMSCs within the “Transition” formulation displayed a phenotypic profile similar to hypertrophic chondrocytes, with the highest expression of collagen X, intermediate levels of osteopontin, and mineralization levels equivalent to “bone” formulations. Overall, these results suggest that a graded transition from PEG-CSC-TGFβ1 to PEG-PDMS-BMP2 scaffolds elicits a gradual SMSC phenotypic shift from chondrocyte to hypertrophic chondrocyte to osteoblast-like. As such, further development of these scaffold formulations for use in SMSC-based OCD repair is warranted.

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Hyaluronic acid facilitates chondrogenesis and matrix deposition of human adipose derived mesenchymal stem cells and human chondrocytes co-cultures

Cited by 100 publications

References 58 publications

Autologous bioscaffolds based on different concentrations of platelet rich plasma and synovial fluid as a vehicle for mesenchymal stem cells

Autologous bioscaffolds based on different concentrations of platelet rich plasma and synovial fluid as a vehicle for mesenchymal stem cells

Optimization of photocrosslinked gelatin/hyaluronic acid hybrid scaffold for the repair of cartilage defect

Toward zonally tailored scaffolds for osteochondral differentiation of synovial mesenchymal stem cells

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