Hyaluronic Acid/Poly‐<scp>l</scp>‐Lysine Multilayers as Reservoirs for Storage and Release of Small Charged Molecules

Prokopović, Vladimir Z.; Duschl, Claus; Volodkin, Dmitry

doi:10.1002/mabi.201500093

Cited by 25 publications

(32 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This means the same amount of Rho was found for PLL or HA terminated film. This shows that the binding of Rho to negatively charged HA within the film is independent of the nature of the terminating layer due to the differences in the diffusion of HA and PLL . However, CF loading is lower in the case of films terminated with HA compared to PLL terminated one.…”

Section: Use Of Ha and Its Derivatives In Coatings Polyelectrolyte Mmentioning

confidence: 98%

“…This can be explained by the lower free PLL quantity in the films terminated with HA, as a result of PLL diffusion. Finally, films terminated with HA present lower quantity of free positive PLL charges …”

Section: Use Of Ha and Its Derivatives In Coatings Polyelectrolyte Mmentioning

confidence: 98%

“…The multilayers obtained by PLL and hyaluronic acid (HA) have been shown to be a good candidate to act as a reservoir for small molecules (particularly charged molecules) and their release. The adsorption and the release mechanisms are related to the diffusion on the film surface …”

Section: Use Of Ha and Its Derivatives In Coatings Polyelectrolyte Mmentioning

confidence: 99%

See 2 more Smart Citations

Hyaluronic Acid and Its Derivatives in Coating and Delivery Systems: Applications in Tissue Engineering, Regenerative Medicine and Immunomodulation

Knopf‐Marques

Pravda

Wolfová

et al. 2016

Adv Healthcare Materials

193

159

View full text Add to dashboard Cite

epithelial, connective, and nerve tissues of vertebrates. It is designated as a glycosaminoglycan. [1] Glycosaminoglycans are composed of disaccharide blocks of N-acetylgalactosamine or N-acetylglucosamine, (amino sugars) and uronic sugars such as glucuronic acid, iduronic acid or galactose. This group of heteropolysaccharides comprises HA, chondroitin sulfates, dermatan sulfate, heparin and heparin sulfates whose sulfation degree is less than that of heparin. Unlike other glycosaminoglycans, hyaluronan is not sulfated and it is self-standing, i.e. without an association with a core protein. [2] It was isolated in 1934 by Meyer and Palmer from bovine vitreous humor. [3] The polymer chain of hyaluronan comprises repeating disaccharide units where the pyranose rings are connected by β-1,3 bonds. The repeating units are bonded with a β-1,4 glycosidic bond within the chain between N-acetyl-D-glucosamine and D-glucuronic acid. At physiological pH each glucuronate unit, associated with its carboxylate group, carries an anionic charge. The hundreds of negative charges are fixed to each chain. These anionic groups are balanced with mobile cations such as Na + , K + , Ca 2+ and Mg 2+ . During ionization of D-glucuronic acid with the carboxylic groups, the charges influence the organization of the chains and their interactions with their surroundings. In turn, they are affected by pH and ionic strength. The chain organization and charge directly affect the solubility in water, since hyaluronan is water-insoluble when convertedAs an Extracellular Matrix (ECM) component, Hyaluronic acid (HA) plays a multi-faceted role in cell migration, proliferation and differentiation at micro level and system level events such as tissue water homeostasis. Among its biological functions, it is known to interact with cytokines and contribute to their retention in ECM microenvironment. In addition to its biological functions, it has advantageous physical properties which result in the industrial endeavors in the synthesis and extraction of HA for variety of applications ranging from medical to cosmetic. Recently, HA and its derivatives have been the focus of active research for applications in biomedical device coatings, drug delivery systems and in the form of scaffolds or cell-laden hydrogels for tissue engineering. A specific reason for the increase in use of HA based structures is their immunomodulatory and regeneration inducing capacities. In this context, this article reviews recent literature on modulation of the implantable biomaterial microenvironment by systems based on HA and its derivatives, particularly hydrogels and microscale coatings that are able to deliver cytokines in order to reduce the adverse immune reactions and promote tissue healing.

show abstract

Section: Use Of Ha and Its Derivatives In Coatings Polyelectrolyte Mmentioning

confidence: 98%

Section: Use Of Ha and Its Derivatives In Coatings Polyelectrolyte Mmentioning

confidence: 98%

See 1 more Smart Citation

Hyaluronic Acid and Its Derivatives in Coating and Delivery Systems: Applications in Tissue Engineering, Regenerative Medicine and Immunomodulation

Knopf‐Marques

Pravda

Wolfová

et al. 2016

Adv Healthcare Materials

193

159

View full text Add to dashboard Cite

show abstract

“…Improved cellular adhesion ( Figure 1F and Figure S2) compared to uncoated multilayers correlates well with our previous results showing that better adhesion of cells was attributed to multilayer stiffening induced through the presence of nanoparticles on the multilayer surface. [26][27] Hence, the coated multilayers become not only cell-adhesive but also resistant to biodegradation, as indicated by a constant width of the scratch during and after cellular growth ( Figure 1F). One can assume that the coating acts as a barrier and prevents diffusion of cell-expressed enzymes into the multilayers and, as a result, suppresses multilayer biodegradation.…”

mentioning

confidence: 96%

Biodegradation-Resistant Multilayers Coated with Gold Nanoparticles. Toward a Tailor-made Artificial Extracellular Matrix

Prokopović

Vikulina

Sustr

et al. 2016

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

Abstract:Polymer multicomponent coatings such as multilayers mimic extracellular matrix (ECM) that attracts significant attention for their use as functional supports for advanced cell culture and tissue engineering. Herein, biodegradation and molecular transport in hyaluronan/polylysine multilayers coated with gold nanoparticles was described. Nanoparticle 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 2 coating acts as semipermeable barrier that governs molecular transport into/from the multilayers and makes them biodegradation resistant. Model protein lysozyme (mimics of ECM soluble signals) diffuses in the multilayers as fast and slow diffusing populations existing in an equilibrium. Such composite system may have high potential to be exploited as degradationresistant drug delivery platforms suitable for cell-based applications.The extracellular matrix (ECM) provides not only a structural support for cellular growth, but also a biochemical milieu, which together define cell fate. 1 Artificial ECM is designed to mimic real ECM and to achieve cellular functions in laboratory designed materials similar as in nature.Artificial ECM consists of degradable supports made of natural or (semi)synthetic polymers which: i) recapitulate physical-chemical properties of real ECM, ii) host bioactive signaling molecules (e.g. hormones, cytokines, growth factors) to be presented to cells, and iii) possess tuned biodegradability. 2-3 The issues above are challenging for the fabrication of artificial ECM but are essential for successful applications in tissue engineering, diagnostics, and animal-free studies.Introduced in the early nineties, 4 layer-by-layer deposition proofed to be extremely useful in the field of bio-functional coatings. It is powerful technique for the fabrication of polymer-based multilayer films with dimensions from nano to micro, adjusted composition, and tunable cellular response. [5][6][7][8][9][10][11] Control over cellular functions such as adhesion, differentiation, and proliferation, 12-15 along with reservoir properties for a variety of biomolecules such as proteins, growth factors, genes, and drugs 7, 16-20 makes these films an attractive platform for cell-based applications.Despite the evident biological potential to employ the multilayers to recapitulate ECM, 3 the options to tune multilayer biodegradability are mostly limited to multilayer chemical 13,[21][22] that can significantly change multilayer properties. There is also a lack in understanding the mechanism of biomolecule-multilayer interaction that obviously defines biomolecule presentation/release from multilayers to cells and, as a result, cellular response. [23][24] In this work a new approach for the design of artificial ECM with controlled biodegradation based on the coating of multilayers with gold nanoparticles (GNPs) is present. The multilayers wer...

show abstract

“…In the work of Prokopovic et al, multilayers of hyaluronic acid and poly-L-lysine act as high-capacity reservoirs for small charged molecules such as rhodamine (positively charged), ATP and carboxyfluoresceine (negatively charged) [101]. HA coating have been exploited for the migration of mesenchymal stem cells due to the well-known interaction between HA and its receptor on MSC membrane.…”

Section: Hyaluronic Acid Coatingsmentioning

confidence: 99%

Development of hyaluronic acid derivatives for applications in biomedical engineering

Petta¹

View full text Add to dashboard Cite

Hyaluronic Acid/Poly‐l‐Lysine Multilayers as Reservoirs for Storage and Release of Small Charged Molecules

Cited by 25 publications

References 50 publications

Hyaluronic Acid and Its Derivatives in Coating and Delivery Systems: Applications in Tissue Engineering, Regenerative Medicine and Immunomodulation

Hyaluronic Acid and Its Derivatives in Coating and Delivery Systems: Applications in Tissue Engineering, Regenerative Medicine and Immunomodulation

Biodegradation-Resistant Multilayers Coated with Gold Nanoparticles. Toward a Tailor-made Artificial Extracellular Matrix

Development of hyaluronic acid derivatives for applications in biomedical engineering

Contact Info

Product

Resources

About