Hyaluronidase-1 Is Mainly Functional in the Upper Granular Layer, Close to the Epidermal Barrier

Malaisse, Jérémy; Evrard, C.; Féret, Damien; Colombaro, Vanessa; Dogné, Sophie; Haftek, Marek; Rouvroit, Catherine Lambert de; Flamion, Bruno; Poumay, Yves

doi:10.1038/jid.2015.299

Cited by 11 publications

(17 citation statements)

References 18 publications

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“…The low levels of Hyal1 mRNA and protein in the organotypic REK cultures, and appearance of the HYAL1 protein late in maturation, are in line with its strict location in the granular layer, where it has been suggested to act as a gatekeeper, restricting high‐molecular‐mass hyaluronan entering the stratum corneum …”

Section: Discussionmentioning

confidence: 63%

“…The hyaluronidases (HYAL) 1 and 2 are expressed in the epidermis . HYAL1 probably degrades hyaluronan in the granular cells, whereas the role of HYAL2 is poorly understood. There are no reports concerning the epidermal expression of KIAA1199 (CEMIP), which is involved in hyaluronan degradation by fibroblasts .…”

mentioning

confidence: 99%

“…The epidermal staining pattern of the hyaluronan receptor CD44 correlates with that of hyaluronan, suggesting their co‐regulation and a role for CD44 in hyaluronan turnover . The hyaluronidases (HYAL) 1 and 2 are expressed in the epidermis . HYAL1 probably degrades hyaluronan in the granular cells, whereas the role of HYAL2 is poorly understood.…”

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confidence: 99%

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Hyaluronan metabolism enhanced during epidermal differentiation is suppressed by vitamin C

et al. 2018

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Section: Discussionmentioning

confidence: 63%

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confidence: 99%

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Hyaluronan metabolism enhanced during epidermal differentiation is suppressed by vitamin C

et al. 2018

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“…Our attempts to ultrastructurally localize HA using HABP failed to reveal this GAG either in the extracellular spaces of SC or at the surface of native and delipidated corneocytes (data not shown). HA is mostly processed by hyaluronidase 1 during the transition between SG and SC but is not completely degraded. In fact, Sakai et al .…”

Section: Discussionmentioning

confidence: 99%

Cell surface glycans in the human stratum corneum: distribution and depth‐related changes

Abdayem

Formanek

Minondo

et al. 2016

Experimental Dermatology

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During the formation of the stratum corneum (SC) barrier, the extracellular spaces of viable epidermis, rich in glycans, are filled with a highly organized lipid matrix and the plasma membranes of keratinocytes are replaced by cornified lipid envelopes. These structures comprise cross‐linked proteins, including transmembrane glycoproteins and proteoglycans, covalently bound to a monolayer of cell surface ceramides. Little is known about the presence and distribution of glycans on the SC corneocytes despite their possible involvement in SC hydration, cohesion and desquamation. In this work, we visualized ultrastructurally and quantified the distribution of glycans on the surface of native and delipidated corneocytes. The cells were harvested at different depths of the SC, allowing us to define the relationship between the distribution of various glycans, proteoglycans and glycoproteins, and other changes occurring in SC. At the cell periphery, we found a correlation between the depth‐related alterations of corneodesmosome glycoproteins and α‐d‐mannosyl and N‐acetyl‐d‐glucosamine‐labelling patterns. Elimination of the terminal sugars, α‐linked fucose and α‐(2,3) linked sialic acid, was less abrupt, but also the initial extent of their peripheral distribution was overall lower than that of concanavalin A and wheat germ agglutinin lectin‐detected glycans. Diffuse labelling of heparan sulphate glycosaminoglycans disappeared completely from the outermost corneocytes, whereas that of several simple carbohydrates could be detected at all SC levels. Our results suggest that specific glycan distribution may participate in the progressive changes of SC, as it evolves from the SC compactum to the SC disjunctum, towards desquamation.

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“…[12] Various types of hyaluronidases exist in nature, HYAL1 being the predominant one in the ECM, expressed in keratinocytes and fibroblasts of human skin. [13] HYAL1 is involved in biological processes such as angiogenesis [14] and skin photo-ageing. [15] We have previously designed HA-based micelles and vesicles resulting from the controlled self-assembly of amphiphilic copolymers.…”

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confidence: 99%

Hyaluronic‐Acid‐Presenting Self‐Assembled Nanoparticles Transform a Hyaluronidase HYAL1 Substrate into an Efficient and Selective Inhibitor

et al. 2020

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In this study, an original method of macromolecular design was used to develop a hyaluronidase‐1 (HYAL1) inhibitor from its principal substrate, hyaluronic acid (HA). HA‐based nanoparticles (HA‐NP) were obtained by copolymer self‐assembly and their effects on HYAL1 activity were investigated by combining different analytical tools. Compared to HA, HA‐NP exhibited an enhanced stability against HYAL1 degradation while maintaining its interaction with the HA receptors CD44 and aggrecan. HA‐NP displayed a strong and selective inhibition of HYAL1 activity and retarded the hydrolysis of higher‐molar‐mass HA in solution. A co‐nanoprecipitation process was used to formulate a range of hybrid nanoparticle samples, which demonstrated the specificity and efficiency of HA‐NP in HYAL1 inhibition.

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Hyaluronidase-1 Is Mainly Functional in the Upper Granular Layer, Close to the Epidermal Barrier

Cited by 11 publications

References 18 publications

Hyaluronan metabolism enhanced during epidermal differentiation is suppressed by vitamin C

Hyaluronan metabolism enhanced during epidermal differentiation is suppressed by vitamin C

Cell surface glycans in the human stratum corneum: distribution and depth‐related changes

Hyaluronic‐Acid‐Presenting Self‐Assembled Nanoparticles Transform a Hyaluronidase HYAL1 Substrate into an Efficient and Selective Inhibitor

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