Hyaluronidase-inhibitory activities of glycosaminoglycans from Liparis tessellatus eggs

Abstract

Polysaccharide fractions isolated from L. tessellatus eggs were purified and eluted using the DEAE-sepharose fast flow column. These were collected, tested and pooled based on their sugars content: F1, F2, and F3 which contain 26.8, 23.3, and 20.2% sulfated glycans; 34.5, 38.2, and 45.0% uronic acids; and 23.5, 19.0, and 7.5% acetylhexosamines and hexosamines, respectively. Hyaluronidase inhibitory effects of the fractions are in the order F3>F2>F1>Ascorbic acid, with F3 having the highest inhibition among the fractions and that of the standard, ascorbic acid. The ESI-MS/MS confirmed the presence of uronic acids on F3, which could be a 0,2 A2 fragment plus loss of methyl group which is very common among nonmethylated, sulfated disaccharides.

Antioxidant and anti-inflammatory activities of phenolic compounds grafted with hyaluronic aicd derived from Liparis tessellatus eggs

“…The F2 and F3 fractions contained 20.2% to 26.8% sulfated GAGs, 34.5% to 45.0% uronic acids, and 7.5% to 23.5% hexosamines. Electron spray ionization-mass spectroscopy confirmed the composition of the F3 fraction to be mainly HA, as reported by Ticar et al (2017).…”

Extraction Optimization, Characterization and Biological Activities of Polysaccharide Extracts from Nymphaea hybrid

“…Hyaluronidase activity inhibition is usually used to assess the anti-inflammatory and anti-allergenic effects of active substances [59]. Hyaluronic acid plays a significant role in tissue regeneration, including skin healing and wound repair, inflammation response and angiogenesis [60]. In this study, the hyaluronidase inhibition rate increased obviously with the increase in the sample concentration indicating that the hyaluronidase inhibition ability was concentration-dependent (Figure 7).…”

In vitro anti-inflammatory and comparative cytotoxicity studies on methanolic extract of Enicostemma hyssopifolium

“…In order to arrest its activity, agents such as hyaluronidase are found to be profoundly effective which could eventually hamper the allergic and inflammation reactions. The hyaluronidase assay measures the amount of N-acetyl glucosamine produced due to the degradation of HA by the breakage of glycosidic bond (Mio et al, 2000;Nawy et al, 2001;Ticar et al, 2017). The present work was carried out using E. hyssopifolium methanolic extract with increasing concentrations to determine its effect in inhibiting the hyaluronidase activity.…”