Hyaluronidase-, β-Glucuronidase- und β-Acetylglucosaminidase-Aktivität im menschlichen Serum während der Schwangerschaft

Platt, D.; Platt, M.

doi:10.1007/bf01745491

Cited by 10 publications

(4 citation statements)

References 21 publications

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“…Serum 3-glucuronidase (EC 3.2.1.31), hyaluronidase (EC 3.2.1.35) and 3-acetylglucosaminidase (EC 3.2.1.30) have all been described as increasing late in normal pregnancy (Platt and Platt, 1968). 'The Apgar score is based on assessment of the baby's colour, heart rate, respirations, muscle tone, and the response to a catheter in the nostril.…”

mentioning

confidence: 99%

Enzyme assays in the management of pregnancy.

Curzen¹

1970

Journal of Clinical Pathology

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confidence: 99%

Enzyme assays in the management of pregnancy.

Curzen¹

1970

Journal of Clinical Pathology

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“…In mice there is a rise and fall in placental glycosaminoglycans (7) and sex hormones can induce changes in the glycos aminoglycan content of both ordinary skin (6) and sexual skin (8). During pregnancy there is also a rise in maternal serum hyaluronidase ac tivity (2,5). The increased turnover of these macromolecules would be expected to result in increased levels of their breakdown products in the circulation.…”

Section: Introductionmentioning

confidence: 99%

Glycosaminoglycan Metabolism in Pregnancy

Salkie¹,

Hannah²

1977

Enzyme

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An unselected group of pregnant patients was followed during pregnancy. Serial assays were made of maternal serum levels of hyaluronidase activity, free amino sugar and uronic acid. It was found that there was a highly significant correlation between the serum levels of hyaluronidase activity and uronic acid, both of which rose during pregnancy. In normal pregnancy the maternal serum amino sugar level showed a moderately significant correlation with length of gestation, which was not shown in abnormal pregnancies. Conversely the rise in uronic acid level with length of gestation was most significant in patients with complicated pregnancies.

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“…Plazenten, Eihäute und Nabelschnüre wur den zunächst mechanisch zerkleinert. Ein Teil diente zur DNS-Bestimmung [11,12]. Der Rest wurde mittels eines Ultraturrax in der 3fachen Menge physiologischer Kochsalzlösung homogenisiert und anschliessend bei -25°C eingefroren.…”

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“…Der Rest wurde mittels eines Ultraturrax in der 3fachen Menge physiologischer Kochsalzlösung homogenisiert und anschliessend bei -25°C eingefroren. Nach Auftauen der Gewebsproben und zweimaligem erneutem Einfrieren und Auftauen wur den die Homogenate in der Kühlzentrifuge ( +4°C) 20 Minuten lang bei 6000 U/min zentrifugiert und im Homogenatübcrstand Enzymaktivitäts-und Proteinmessung [11,12] 1. Eine verm ehrte Ausschwemmung der Hyaluronidase, ß-Glukuronidase und ß-Azetylglukosaminidasc aus der Plazenta.…”

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