Animation techniques have been completely transformed by the union of Artificial Intelligence (AI) and biomechanical modeling, particularly in 2D animation. This study looks at a combination of AI and biomechanics to address the challenges of simulating 2D animation. Current approaches in 2D animation often struggle to achieve lifelike and fluid movements, especially when representing complex motion or interaction. These traditional techniques rely on manual keyframing or physics simulation, which may be time-consuming and do not provide the rich detail needed for realism in animations. To meet these aspects, this study suggested 2D animation using Artificial Intelligence with Biomechanical Modeling (2D-AI-BM). Our approach thus harnesses Deep Neural Network (DNN) for moving forecasts and improvement using biopsychological principles to help us imitate natural human actions better. In addition to character animation, it could apply to interactive storytelling and educational simulations. As a result, animators get more control over motion generation while drastically reducing the necessity for manual intervention through this fusion of AI and biomechanics, which smoothens the production pipeline for animations. This paper considers several important metrics to evaluate the proposed approach’s effectiveness, including user satisfaction, computational efficiency, motion smoothness and realism. Comparative studies with classical animation methods showed that the method generates realistic movements on 2D characters while saving time during production. The numerical findings exemplify that the recommended 2D-AI-BM model improves an accuracy rate of 97.4%, computational efficiency ratio of 96.3%, motion control ratio of 95.4%, pose detection ratio of 94.8% and scalability ratio of 93.2% compared to other popular techniques.