Hybrid Biodegradable Polymeric Scaffolds for Cardiac Tissue Engineering

El-Husseiny, Hussein M.; Mady, Eman A.; Radwan, Yasmine; Nagy, Maria; Abugomaa, Amira; Elbadawy, Mohamed; Tanaka, Ryou

doi:10.1007/978-3-031-09710-2_48

Cited by 3 publications

(3 citation statements)

References 95 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, these materials can trigger a more severe immune response compared to traditional materials. Biodegradable materials, such as polymers, are also being explored as an alternative to permanent foreign materials [ 117 ]. They can gradually degrade over time and be replaced by host tissue, minimizing the foreign body reaction [ 26 ].…”

Section: Impact Of the Foreign Body Reaction On Spinal Fusionmentioning

confidence: 99%

Immune response to foreign materials in spinal fusion surgery

Cai,

Wang,

Cai

et al. 2023

Heliyon

View full text Add to dashboard Cite

Section: Impact Of the Foreign Body Reaction On Spinal Fusionmentioning

confidence: 99%

Immune response to foreign materials in spinal fusion surgery

Cai,

Wang,

Cai

et al. 2023

Heliyon

View full text Add to dashboard Cite

“…Recent breakthroughs in biomedicine and tissue regeneration have significantly broadened the scope of healthcare [ 1 , 2 , 3 ]. Over the years, a wide range of therapeutic modalities and materials have been employed [ 4 , 5 , 6 , 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Impact of Adipose Tissue Depot Harvesting Site on the Multilineage Induction Capacity of Male Rat Adipose-Derived Mesenchymal Stem Cells: An In Vitro Study

El-Husseiny

Kaneda

Mady

et al. 2023

IJMS

View full text Add to dashboard Cite

Recently, substantial attention has been paid toward adipose-derived mesenchymal stem cells (AdMSCs) as a potential therapy in tissue engineering and regenerative medicine applications. Rat AdMSCs (r-AdMSCs) are frequently utilized. However, the influence of the adipose depot site on the multilineage differentiation potential of the r-AdMSCs is still ambiguous. Hence, the main objective of this study was to explore the influence of the adipose tissue harvesting location on the ability of r-AdMSCs to express the stem-cell-related markers and pluripotency genes, as well as their differentiation capacity, for the first time. Herein, we have isolated r-AdMSCs from the inguinal, epididymal, peri-renal, and back subcutaneous fats. Cells were compared in terms of their phenotype, immunophenotype, and expression of pluripotency genes using RT-PCR. Additionally, we investigated their potential for multilineage (adipogenic, osteogenic, and chondrogenic) induction using special stains confirmed by the expression of the related genes using RT-qPCR. All cells could positively express stem cell marker CD 90 and CD 105 with no significant in-between differences. However, they did not express the hematopoietic markers as CD 34 and CD 45. All cells could be induced successfully. However, epididymal and inguinal cells presented the highest capacity for adipogenic and osteogenic differentiation (21.36-fold and 11.63-fold for OPN, 29.69-fold and 26.68-fold for BMP2, and 37.67-fold and 22.35-fold for BSP, respectively, in epididymal and inguinal cells (p < 0.0001)). On the contrary, the subcutaneous cells exhibited a superior potential for chondrogenesis over the other sites (8.9-fold for CHM1 and 5.93-fold for ACAN, (p < 0.0001)). In conclusion, the adipose tissue harvesting site could influence the differentiation capacity of the isolated AdMSCs. To enhance the results of their employment in various regenerative cell-based therapies, it is thus vital to take the collection site selection into consideration.

show abstract

“…The current scarcity of human organs and tissues appropriate for life-saving transplants is expected to persist in the future despite the great endeavors to close the donation gap [1][2][3][4][5][6]. Hence, it is highly crucial to introduce efficient alternatives to avoid the high incidence of deaths among patients that occur due to the long waiting lists for donor organs/tissues or due to receiving suboptimal treatments [7][8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Bovine- and Porcine-Derived Decellularized Biomaterials: Promising Platforms for Tissue Engineering Applications

et al. 2023

View full text Add to dashboard Cite

Animal-derived xenogeneic biomaterials utilized in different surgeries are promising for various applications in tissue engineering. However, tissue decellularization is necessary to attain a bioactive extracellular matrix (ECM) that can be safely transplanted. The main objective of the present study is to assess the structural integrity, biocompatibility, and potential use of various acellular biomaterials for tissue engineering applications. Hence, a bovine pericardium (BP), porcine pericardium (PP), and porcine tunica vaginalis (PTV) were decellularized using a Trypsin, Triton X (TX), and sodium dodecyl sulfate (SDS) (Trypsin + TX + SDS) protocol. The results reveal effective elimination of the cellular antigens with preservation of the ECM integrity confirmed via staining and electron microscopy. The elasticity of the decellularized PP (DPP) was markedly (p < 0.0001) increased. The tensile strength of DBP, and DPP was not affected after decellularization. All decellularized tissues were biocompatible with persistent growth of the adipose stem cells over 30 days. The staining confirmed cell adherence either to the peripheries of the materials or within their matrices. Moreover, the in vivo investigation confirmed the biocompatibility and degradability of the decellularized scaffolds. Conclusively, Trypsin + TX + SDS is a successful new protocol for tissue decellularization. Moreover, decellularized pericardia and tunica vaginalis are promising scaffolds for the engineering of different tissues with higher potential for the use of DPP in cardiovascular applications and DBP and DPTV in the reconstruction of higher-stress-bearing abdominal walls.

show abstract

Hybrid Biodegradable Polymeric Scaffolds for Cardiac Tissue Engineering

Cited by 3 publications

References 95 publications

Immune response to foreign materials in spinal fusion surgery

Immune response to foreign materials in spinal fusion surgery

Impact of Adipose Tissue Depot Harvesting Site on the Multilineage Induction Capacity of Male Rat Adipose-Derived Mesenchymal Stem Cells: An In Vitro Study

Comparison of Bovine- and Porcine-Derived Decellularized Biomaterials: Promising Platforms for Tissue Engineering Applications

Contact Info

Product

Resources

About