Hybrid Bionanocomposite Containing Magnesium Hydroxide Nanoparticles Embedded in a Carboxymethyl Cellulose Hydrogel Plus Silk Fibroin as a Scaffold for Wound Dressing Applications

Abstract

Based on the promising biomedical developments in wound healing strategies, herein, a new nanobiocomposite scaffold was designed and presented by incorporation of carboxymethyl cellulose hydrogels prepared using epichlorohydrin as a cross-linking agent (CMC hydrogel), a natural silk fibroin (SF) protein, and magnesium hydroxide nanoparticles (Mg(OH) 2 NPs). Biological evaluation of the CMC hydrogel/SF/Mg(OH) 2 nanobiocomposite scaffold was conducted via in vitro cell viability assays and in vivo assays, red blood cell hemolysis, and antibiofilm assays. Considering the cell viability percentage of Hu02 cells (84.5%) in the presence of the prepared nanobiocomposite after 7 days, it was indicated that this new nanoscaffold was biocompatible. The signs of excellent hemocompatibility and the high antibacterial activity were observed due to the low-point hemolytic effect (8.3%) and high-level potential in constraining the P. aeruginosa biofilm formation with a low OD value (0.13). Moreover, in vivo wound healing assay results indicated that the wound healing method was faster in mice treated with the prepared nanobiocomposite scaffold (82.29%) than the control group (75.63%) in 12 days. Apart from the structural characterization of the CMC hydrogel/SF/Mg(OH) 2 nanobiocomposite through FTIR, EDX, FESEM, and TG analyses, compressive mechanical tests, contact angle, porosity, and swelling ratio studies indicated that the combination of the CMC hydrogel structure with SF protein and Mg(OH) 2 NPs could significantly impact Young's modulus (from 11.34 to 10.14 MPa), tensile strength (from 299.35 to 250.78 MPa), elongation at break (12.52 to 12.84%), hydrophilicity, and water uptake capacity (92.5%).

A Mg2+/polydopamine composite hydrogel for the acceleration of infected wound healing

“…Similarly, the survival rate of E. coli treated with PAM hydrogel was 87.67%, much higher than those of bacteria treated with PAM-PDA (8.09%) and PDA-PAM/Mg 2+ (7.06%). Owing to Mg 2+ -synergized photothermal antibacterial activity under NIR irradiation, the PDA-PAM/Mg 2+ hydrogel showed a lower survival rate (<10%) than the previously reported Mg 2+ -based carboxymethyl cellulose (CMC) and silk fibroin (SF) composite hydrogel (CMC/SF/Mg(OH) 2 , approximately 20%) [ 34 ].…”

A novel, bioactive and antibacterial scaffold based on functionalized graphene oxide with lignin, silk fibroin and ZnO nanoparticles

“…To measure the anti-biofilm properties of the nanobiocomposite, both samples were stained by 0.1% crystal violet solution for 5 min and then washed by 33% acetic acid solution to separate the bacteria from their surface . Finally, using a microplate reader (STAT FAX 2100, BioTek, Winooski, USA) , the absorbance of the resulting solutions was evaluated at 570 nm 16 , 48 .…”

“…This materials are used in various fields including remediation of heavy metals 6 , sensor 7 , food packaging 8 , catalyst and organic synthesis 9 – 12 , and adsorption of textile dye 13 . Furthermore, nanobiocomposites with properties such as nontoxicity, hemocompatibility, and improved mechanical properties are appropriate candidates for utilizing in biological applications and in this regard, various reports have been presented in fields of drug delivery 14 , pharmaceutics 15 , wound healing 16 , antibacterial materials 17 , cancer therapy 3 , 18 , 19 , and tissue engineering 20 . A wide range of materials has been used to synthesize biological nanobiocomposites.…”

Modified copper zinc ferrite nanoparticles doped with Zr ions for hyperthermia applications

“…Biological evaluation of the CMC hydrogel/SF/Mg(OH) 2 nanobiocomposite scaffold was conducted via in vitro cell viability assays and in vivo assays, red blood cell hemolysis, and antibiofilm assays. From the results, the CMC hydrogel/SF/Mg(OH) 2 nanobiocomposite could be considered a new candidate for further investigation in other biomedical fields like tissue engineering [22]. A new magnetic nanocomposite (CuFe 2 O 4 MNPs) with a statistical star polymer structure was designed and synthesized.…”