Hybrid detection of target sequence DNA based on phosphorescence resonance energy transfer

A label-free RTP sensor based on aptamer/quantum dot nanocomposites for cytochrome c detection

“…Quantum dots (QDs) are a novel type of optical nanomaterials that possess high application prospects. Owing to their large Stokes shi, narrow emission spectrum and high luminescence efficiency, QDs have been commonly applied into DNA molecular hybridization 25,26 and into detection of small molecules, 27,28 proteins 29,30 and enzymatic activity. 31,32 Roomtemperature phosphorescent (RTP) QDs possess longer lifetime and much rare phosphorescence and thus can avoid the interference from the background uorescence of biological systems or from the autouorescence and scattering light of biological samples.…”

Preparation of DNA functional phosphorescent quantum dots and application in melamine detection in milk

“…QDs 1-3 feature favorable biocompatibility, low toxicity and degradability, etc., they are of important application value in elds like biomolecular sensing, 4 environmental pollutant detection 5 and biomedicine, 6 so they have been extensively applied in practice. 7,8 Especially DNA molecules [9][10][11] have many advantages, including favorable thermal stability and an electronegative pentose-phosphate backbone which can easily bond with metal ions. [12][13][14] Moreover, they will not harm the environment aer degradation and can articially synthesize DNAs of specic sequences and form many aptamers of different sequences on QDs surfaces, which can contribute to preparation of multiple kinds of discriminatory analysis sensors of good selectivity.…”

-Phosphorescent Mesoporous Surface Imprinting Microspheres: Preparation and Application for Transferrin Recognition from Biological Fluids

“…Moreover, the latent high biotoxicity of Cd 2+ -containing QDs complicates the use of fluorescent QD surface MIPs in protein analysis in real biological samples. Nevertheless, these limitations are overcome by Mn-doped ZnS (Mn–ZnS) QDs with room-temperature phosphorescence (RTP) because the interference from background fluorescence and scattered light in biological samples can be avoided during RTP detection − and RTP is relatively less common than fluorescence, which further improves the selective specificity of the RTP detection. The phosphorescent QD-based RTP detection avoids the addition of any deoxidant or inductive agent and the complicated pretreatment of biological samples. − Until now, the excellent RTP property of Mn–ZnS QDs has been successfully used to develop various sensors. ,,− …”