Hybrid hydrogel photonic barcodes for multiplex detection of tumor markers

Abstract

Barcodes-based suspension array have for demonstrated values in multiplex assay of tumor markers. Photonic barcodes which are encoded by their characteristic reflection peaks are the important supports for suspension array due to their stable code, low fluorescent background and high surface-volume ratio. Attempts to develop this technology tend to improve the function of the photonic barcodes. Here, we present a new type of hybrid hydrogel photonic barcodes for efficient multiplex assays. This photonic barcodes are hybrid inverse opal hydrogel composed of poly(ethylene glycol) diacrylate (PEG-DA) and agarose. The polymerized PEG-DA hydrogel could guarantee the stabilities of the inverse opal structure and its resultant code, while the agarose could offer active chemical groups for the probe immobilization and homogeneous water surrounding for the bioassay. In addition, the interconnected pores inverse opal structure could provide channels for biomolecules diffusing and reaction into the voids of barcodes. These features imparted the hybrid hydrogel photonic barcodes with limits of detection (LOD) of 0.78ng/mL for carcinoembryonic antigen (CEA) and 0.21ng/mL for α-fetoprotein (AFP), respectively. It was also demonstrated that the proposed barcodes showed acceptable accuracy and detection reproducibility, and the results were in acceptable agreement with those from common clinic method for the detections of practical clinical samples. Thus, our technique provides a new platform for simultaneous multiplex immunoassay.

Responsive principles and applications of smart materials in biosensing

“…Moreover, Zhao group developed porous hydrogels encapsulated with PCs for multiplex detections of proteins, such as tumor markers [ 79 ], cardiovascular biomarkers [ 80 ], and skin interstitial fluid (ISF) biomarkers [ 81 ]. In a recent study, arranged PC barcodes were embedded inside a microneedle arrays (MNs) for specific detection of ISF biomarkers [ 81 ].…”

Emerging barcode particles for multiplex bioassays

“…Thus, different high-throughput multiplexed assays can be realized by combining these detection methods with microarrays, while there are still some disadvantages in terms of the way that they are produced and used. Nowadays, due to its fast solution kinetics, large encoding capacity, rapid reaction time and rapid signal acquisition, barcode particles as microcarriers have been used in the multiplex protein detection by combining with different detection methods, such as fluorescence-based, SERSbased and label-free protein detection methods [184,185].…”

Label-free microfluidic paper-based electrochemical aptasensor for ultrasensitive and simultaneous multiplexed detection of cancer biomarkers

“…With the increasingly worsening air pollution over the past decades, lung cancer has been one of the most threatening cancer to people's health and life worldwide (Lin et al 2017;Song et al 2016). The rapid simultaneous detection of multiple cancer biomarkers in body fluids could help confirm lung cancer as soon as possible, so as to provide scientific reference for clinical treatment (Xu et al 2017). However, the determination of a single tumor marker usually has a limiting clinical reference value (Gao et al 2017;Lu et al 2015).…”