Label-free microfluidic paper-based electrochemical aptasensor for ultrasensitive and simultaneous multiplexed detection of cancer biomarkers

Wang, Yang; Luo, Jinping; Liu, Juntao; Sun, Shuai; Xiong, Ying; Ma, Yuanyuan; Yan, Shi; Yang, Yue; Yin, Huabing; Cai, Xinxia

doi:10.1016/j.bios.2019.04.032

Cited by 214 publications

(91 citation statements)

References 37 publications

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“…Even so, analysis of diluted serum samples is still required, thus demanding more sensitive approaches to detect clinically relevant protein levels. Aptamer-based assays challenged to clinical serum samples (from healthy individuals and cancer patients) showed satisfactory agreement with the immunomethod routinely used in hospitals (Wen et al, 2016;Ma et al, 2018a;Liu et al, 2019;Wang et al, 2019b). Nevertheless, their complexity in steps and reagents (Wen et al, 2016;Ma et al, 2018a;Liu et al, 2019) could discourage their implementation in clinical practice.…”

Section: Dna-based Red Diagnosticsmentioning

confidence: 93%

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The Translational Potential of Electrochemical DNA-Based Liquid Biopsy

Miranda‐Castro

Palchetti

de‐los‐Santos‐Álvarez

2020

Front. Chem.

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Latest technological advancement has tremendously expanded the knowledge on the composition of body fluids and the cancer-associated changes, which has fueled the replacement of invasive biopsies with liquid biopsies by using appropriate specific receptors. DNA emerges as a versatile analytical reagent in electrochemical devices for hybridization-based or aptamer-based recognition of all kind of biomarkers. In this mini review, we briefly introduce the current affordable targets (tumor-derived nucleic acids, circulating tumor cells and exosomes) in body fluids, and then we provide an overview of selected electrochemical methods already applied in clinical samples by dividing them into three large categories according to sample type: red (blood), yellow (urine), and white (saliva and sweat) diagnostics. This review focuses on the hurdles of the complex matrices rather than a comprehensive and detailed revision of the format schemes of DNA-based electrochemical sensing. This diverse perspective compiles some challenges that are often forgotten and critically underlines real sample analysis or clinical validation assays. Finally, the needs and trends to reach the market are briefly outlined.

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Section: Dna-based Red Diagnosticsmentioning

confidence: 93%

“…Nevertheless, their complexity in steps and reagents (Wen et al, 2016;Ma et al, 2018a;Liu et al, 2019) could discourage their implementation in clinical practice. Of greater simplicity are signal-off tests (Wang et al, 2019b), although they require very strict selectivity controls.…”

Section: Dna-based Red Diagnosticsmentioning

confidence: 99%

The Translational Potential of Electrochemical DNA-Based Liquid Biopsy

Miranda‐Castro

Palchetti

de‐los‐Santos‐Álvarez

2020

Front. Chem.

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“…The paper-based biosensor was fabricated through wax-and screen-printing. The working electrodes were modified with amino functional graphene (NG)-Thionin (THI)-gold nanoparticles (AuNPs) (NG-THI-AuNPs) and Prussian blue (PB)-poly(3,4-ethylenedioxythiophene) (PEDOT)-AuNPs (PB-PEDOT-AuNPs) nanocomposites, respectively [85]. Experimental results obtained from cyclic voltammetry (CV) and differential pulse voltammetry (DPV) revealed that the aptasensor demonstrated remarkable linearity in ranges of 0.01-500 ng mL −1 for CEA and 0.05-500 ng mL −1 for NSE, respectively, whereas the detection limit was determined to be 2 pg mL −1 for CEA and 10 pg mL −1 for NSE.…”

Section: Voltammetrymentioning

confidence: 99%

“…Experimental results obtained from cyclic voltammetry (CV) and differential pulse voltammetry (DPV) revealed that the aptasensor demonstrated remarkable linearity in ranges of 0.01-500 ng mL −1 for CEA and 0.05-500 ng mL −1 for NSE, respectively, whereas the detection limit was determined to be 2 pg mL −1 for CEA and 10 pg mL −1 for NSE. Therefore, it was shown that paper-based biosensors offer an alternate platform for early cancer diagnostics, especially in resource-limited countries [85].…”

Section: Voltammetrymentioning

confidence: 99%

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Cytokine and Cancer Biomarkers Detection: The Dawn of Electrochemical Paper-Based Biosensor

Loo

Pui

2020

Sensors

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Although the established ELISA-based sensing platforms have many benefits, the importance of cytokine and cancer biomarkers detection for point-of-care diagnostics has propelled the search for more specific, sensitive, simple, accessible, yet economical sensor. Paper-based biosensor holds promise for future in-situ applications and can provide rapid analysis and data without the need to conduct in a laboratory. Electrochemical detection plays a vital role in interpreting results obtained from qualitative assessment to quantitative determination. In this review, various factors affecting the design of an electrochemical paper-based biosensor are highlighted and discussed in depth. Different detection methods, along with the latest development in utilizing them in cytokine and cancer biomarkers detection, are reviewed. Lastly, the fabrication of portable electrochemical paper-based biosensor is ideal in deliberating positive societal implications in developing countries with limited resources and accessibility to healthcare services.

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Aptamer‐Based Biosensors

2024

Biosensors

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Label-free microfluidic paper-based electrochemical aptasensor for ultrasensitive and simultaneous multiplexed detection of cancer biomarkers

Cited by 214 publications

References 37 publications

The Translational Potential of Electrochemical DNA-Based Liquid Biopsy

The Translational Potential of Electrochemical DNA-Based Liquid Biopsy

Cytokine and Cancer Biomarkers Detection: The Dawn of Electrochemical Paper-Based Biosensor

Aptamer‐Based Biosensors

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