Hybrid Materials that Integrate Living Cells: Improved Eco‐Adaptation and Environmental Applications

Xu, Xurong; Wang, Ben; Tang, Ruikang

doi:10.1002/cssc.201100043

Cited by 13 publications

(8 citation statements)

References 103 publications

(123 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[1][2][3][4][5] Traditionally, such modification has primarily involved the physical or chemical conjugation of preformed polymers ('grafting-to', Figure 1a). 1,[6][7][8][9][10][11][12][13][14][15][16][17][18] However, this grafting-to approach is often limited by low polymer grafting on the cell surface and the use of a large excess of reactive polymers for functionalization. 12,19 Direct encapsulation of cells within crosslinked networks has been examined but reduces the modified cell's capability of signal transduction and mass transport due to limited molecular access to the cell surface (Figure 1b).…”

mentioning

confidence: 99%

Engineering live cell surfaces with functional polymers via cytocompatible controlled radical polymerization

et al. 2017

View full text Add to dashboard Cite

The capability to graft synthetic polymers onto the surfaces of live cells offers the potential to manipulate and control their phenotype and underlying cellular processes. Conventional grafting-to strategies for conjugating preformed polymers to cell surfaces are limited by low polymer grafting efficiency. Here we report an alternative grafting-from strategy for directly engineering the surfaces of live yeast and mammalian cells through cell surface-initiated controlled radical polymerization. By developing cytocompatible PET-RAFT (photoinduced electron transfer-reversible addition-fragmentation chain-transfer polymerization), synthetic polymers with narrow polydispersity (M/M < 1.3) could be obtained at room temperature in 5 minutes. This polymerization strategy enables chain growth to be initiated directly from chain-transfer agents anchored on the surface of live cells using either covalent attachment or non-covalent insertion, while maintaining high cell viability. Compared with conventional grafting-to approaches, these methods significantly improve the efficiency of grafting polymer chains and enable the active manipulation of cellular phenotypes.

show abstract

mentioning

confidence: 99%

Engineering live cell surfaces with functional polymers via cytocompatible controlled radical polymerization

et al. 2017

View full text Add to dashboard Cite

show abstract

“…@CaP cells) can be applicable for resisting natural toxins. As extending the storage time is highly favorable in cell engineering, 44 our in situ modification method on living cells would be of great interest to the researchers.…”

Section: Resultsmentioning

confidence: 99%

In Situ Strategy for Biomimetic Construction of Calcium Phosphate Mineral Shells on Microbial Cells

Chen

Zhang

Yang

et al. 2021

ACS Sustainable Chem. Eng.

View full text Add to dashboard Cite

Biomimetic construction of functional shells on cells is highly important in modifying an engineered cell surface. Herein, we report a microbial in situ mineralization strategy using Acetobacter xylinum as a model strain to explore the interactions between cell metabolism and calcium phosphate crystallization. Mineral precipitation has been observed outside the cell walls during the metabolism of bacterial cellulose. Scanning electron microscopy (SEM) and X-ray diffraction (XRD) results reveal that in situ mineralization of calcium phosphate can be realized on a microorganism surface by simply treatment of simulated body fluid. The cell survival test reveals that the prepared artificial mineral shell can be an ideal shield for cells to resist natural toxins without disturbing their normal physiological metabolism. This finding provides a robust route to in situ biomimetic construction of living cells with functional mineral shells during their metabolic process without modifying the surface characteristic.

show abstract

“…However, most organisms cannot self‐biomineralize because they lack relevant molecules for mineralization . The artificial mineralization of materials onto non‐biomineralizing organisms may strengthen the mineralization capability and generate material shells through biomimetic mineralization strategies …”

Section: Biological Strategy For Organism Modificationmentioning

confidence: 99%

Biomineralization: From Material Tactics to Biological Strategy

et al. 2017

Self Cite

View full text Add to dashboard Cite

Biomineralization is an important tactic by which biological organisms produce hierarchically structured minerals with marvellous functions. Biomineralization studies typically focus on the mediation function of organic matrices on inorganic minerals, which helps scientists to design and synthesize bioinspired functional materials. However, the presence of inorganic minerals may also alter the native behaviours of organic matrices and even biological organisms. This progress report discusses the latest achievements relating to biomineralization mechanisms, the manufacturing of biomimetic materials and relevant applications in biological and biomedical fields. In particular, biomineralized vaccines and algae with improved thermostability and photosynthesis, respectively, demonstrate that biomineralization is a strategy for organism evolution via the rational design of organism-material complexes. The successful modification of biological systems using materials is based on the regulatory effect of inorganic materials on organic organisms, which is another aspect of biomineralization control. Unlike previous studies, this study integrates materials and biological science to achieve a more comprehensive view of the mechanisms and applications of biomineralization.

show abstract

Hybrid Materials that Integrate Living Cells: Improved Eco‐Adaptation and Environmental Applications

Cited by 13 publications

References 103 publications

Engineering live cell surfaces with functional polymers via cytocompatible controlled radical polymerization

Engineering live cell surfaces with functional polymers via cytocompatible controlled radical polymerization

In Situ Strategy for Biomimetic Construction of Calcium Phosphate Mineral Shells on Microbial Cells

Biomineralization: From Material Tactics to Biological Strategy

Contact Info

Product

Resources

About