Hybrid microfluidic cartridge formed by irreversible bonding of SU-8 and PDMS for multi-layer flow applications

Talaei, Sara; Frey, Olivier; Wal, P. D. van der; Rooij, N. F. de; Koudelka‐Hep, M.

doi:10.1016/j.proche.2009.07.095

Cited by 23 publications

(15 citation statements)

References 3 publications

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“…The assembled device was kept at 70 C under a force of 30-60 N for 15 min. 19 The devices were removed from the hotplate, and tygon tubing (ID: 0.02 in., OD: 0.06 in.) was inserted into the inlets and outlets of the device.…”

Section: Device Assemblymentioning

confidence: 99%

Microfluidic platform for assessing pancreatic islet functionality through dielectric spectroscopy

et al. 2015

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Human pancreatic islets are seldom assessed for dynamic responses to external stimuli. Thus, the elucidation of human islet functionality would provide insights into the progression of diabetes mellitus, evaluation of preparations for clinical transplantation, as well as for the development of novel therapeutics. The objective of this study was to develop a microfluidic platform for in vitro islet culture, allowing the multi-parametric investigation of islet response to chemical and biochemical stimuli. This was accomplished through the fabrication and implementation of a microfluidic platform that allowed the perifusion of islet culture while integrating real-time monitoring using impedance spectroscopy, through microfabricated, interdigitated electrodes located along the microchamber arrays. Real-time impedance measurements provide important dielectric parameters, such as cell membrane capacitance and cytoplasmic conductivity, representing proliferation, differentiation, viability, and functionality. The perifusion of varying glucose concentrations and monitoring of the resulting impedance of pancreatic islets were performed as proof-of-concept validation of the lab-on-chip platform. This novel technique to elucidate the underlying mechanisms that dictate islet functionality is presented, providing new information regarding islet function that could improve the evaluation of islet preparations for transplantation. In addition, it will lead to a better understanding of fundamental diabetes-related islet dysfunction and the development of therapeutics through evaluation of potential drug effects. V C 2015 AIP Publishing LLC. [http://dx

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Section: Device Assemblymentioning

confidence: 99%

Microfluidic platform for assessing pancreatic islet functionality through dielectric spectroscopy

et al. 2015

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“…2c). 29 The unstructured PDMS layer comprises punched fluidic inlets and outlets, and its surface has to be activated by oxygen plasma (200 Plasma System, TePla AG, Germany) before assembly. It is important to notice, that for the final channel sealing, no precise alignment is required so that it can be performed under a conventional stereomicroscope.…”

Section: Device Fabricationmentioning

confidence: 99%

Microfluidic single-cell cultivation chip with controllable immobilization and selective release of yeast cells

et al. 2012

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We present a microfluidic cell-culture chip that enables trapping, cultivation and release of selected individual cells. The chip is fabricated by a simple hybrid glass-SU-8-PDMS approach, which produces a completely transparent microfluidic system amenable to optical inspection. Single cells are trapped in a microfluidic channel using mild suction at defined cell immobilization orifices, where they are cultivated under controlled environmental conditions. Cells of interest can be individually and independently released for further downstream analysis by applying a negative dielectrophoretic force via the respective electrodes located at each immobilization site. The combination of hydrodynamic celltrapping and dielectrophoretic methods for cell releasing enables highly versatile single-cell manipulation in an array-based format. Computational fluid dynamics simulations were performed to estimate the properties of the system during cell trapping and releasing. Polystyrene beads and yeast cells have been used to investigate and characterize the different functions and to demonstrate biological compatibility and viability of the platform for single-cell applications in research areas such as systems biology. AbstractWe present a microfluidic cell-culture chip that enables the trapping, cultivation and release of selected individual cells. The chip is fabricated by a simple hybrid glass-SU-8-PDMS approach, which produces a completely transparent microfluidic system amenable to optical inspection. Single cells are trapped in a microfluidic channel using mild suction at defined cell immobilization orifices, where

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“…Several earlier reports presented bonding solutions with moderate adhesion between different polymers and PDMS applying subsequent O 2 or N 2 plasma treatments and possible silanisation steps utilising various organosilanes. [19,20,21,22] These bonding experiments were reproduced and evaluated to find an optimal method to seal our photonic and microfluidic subsystems.…”

Section: Microfabricationmentioning

confidence: 99%

PDMS microfluidics developed for polymer based photonic biosensors

et al. 2014

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Abstract:In this work, advances in the fabrication technology and functional analysis of a polymer microfluidic system -as a significant part of a developed polymer photonic biosensor -are reported. Robust and cost-effective microfluidics in PDMS including sample preparation functions is designed and realized by using SU-8 moulding replica. Surface modification strategies using Triton X-100 and PDMS-PEO and their effect on device sealing and non-specific protein adsorption are investigated by contact angle measurement and in situ fluorescence microscopy.Response to Reviewers: Dear Editors and Reviewers, First of all, thank You for the effort in improving our paper. Regarding the reviewer comments, we made the following modifications regarding the latest version of the manuscript submitted. The modifications and the required supplements are submitted in pdf format with the revised manuscript.Thank You again for the efforts to improve our manuscript. Regards, Péter Fürjes

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Hybrid microfluidic cartridge formed by irreversible bonding of SU-8 and PDMS for multi-layer flow applications

Cited by 23 publications

References 3 publications

Microfluidic platform for assessing pancreatic islet functionality through dielectric spectroscopy

Microfluidic platform for assessing pancreatic islet functionality through dielectric spectroscopy

Microfluidic single-cell cultivation chip with controllable immobilization and selective release of yeast cells

PDMS microfluidics developed for polymer based photonic biosensors

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